Phospho-DEPDC5 (Thr1531) Antibody - #AF8607
| 製品: | Phospho-DEPDC5 (Thr1531) Antibody |
| カタログ: | AF8607 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-DEPDC5 (Thr1531) |
| アプリケーション: | WB |
| 反応性: | Human |
| 予測: | Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken |
| 分子量: | 180 kDa; 181kD(Calculated). |
| ユニプロット: | O75140 |
| RRID: | AB_2843463 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8607, RRID:AB_2843463.
折りたたみ／展開
DEP domain containing 5; DEP domain-containing protein 5; DEP.5; DEPD5_HUMAN; DEPDC5; FFEVF; KIAA0645;
免疫原
- O75140 DEPD5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O75140 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T66 | Phosphorylation | Uniprot | |
| Y155 | Phosphorylation | Uniprot | |
| T273 | Phosphorylation | Uniprot | |
| K411 | Methylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| S491 | Phosphorylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| S496 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| S508 | Phosphorylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| S548 | Phosphorylation | Uniprot | |
| S579 | Phosphorylation | Uniprot | |
| T749 | Phosphorylation | Uniprot | |
| Y759 | Phosphorylation | Uniprot | |
| S831 | Phosphorylation | Uniprot | |
| S833 | Phosphorylation | Uniprot | |
| Y920 | Phosphorylation | Uniprot | |
| Y924 | Phosphorylation | Uniprot | |
| S1028 | Phosphorylation | Uniprot | |
| S1530 | Phosphorylation | Uniprot | |
| T1531 | Phosphorylation | Uniprot | |
| Y1546 | Phosphorylation | Uniprot | |
| Y1550 | Phosphorylation | Uniprot | |
| K1574 | Ubiquitination | Uniprot |
研究背景
As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.
Ubiquitinated. Amino acid-induced 'Lys-48'-linked polyubiquitination of DEPDC5 by the BCR(KLHL22) ubiquitin ligase complex leads to DEPDC5 proteasomal degradation and inhibition of the GATOR1 complex. Ubiquitination may occur at multiple lysines.
Cytoplasm>Cytosol. Cytoplasm>Perinuclear region. Lysosome membrane.
Note: Localization to lysosomes is amino acid-independent.
Expressed in developing and adult brain.
Within the GATOR complex, component of the GATOR1 subcomplex, made of DEPDC5, NPRL2 and NPRL3. GATOR1 mediates the strong interaction of the GATOR complex with RRAGA/RRAGC and RRAGB/RRAGC heterodimers. Interacts (via DEP domain) with KLHL22; the interaction depends on amino acid availability.
The DEP domain mediates the interaction with KLHL22.
Belongs to the IML1 family.
研究領域
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
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