Phospho-GDF9 (Tyr394) Antibody - #AF7035
| 製品: | Phospho-GDF9 (Tyr394) Antibody |
| カタログ: | AF7035 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-GDF9 (Tyr394) |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 51kDa; 51kD(Calculated). |
| ユニプロット: | O60383 |
| RRID: | AB_2843475 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7035, RRID:AB_2843475.
折りたたみ／展開
GDF9; Growth differentiation factor 9;
免疫原
Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level).
- O60383 GDF9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARPNKFLLWFCCFAWLCFPISLGSQASGGEAQIAASAELESGAMPWSLLQHIDERDRAGLLPALFKVLSVGRGGSPRLQPDSRALHYMKKLYKTYATKEGIPKSNRSHLYNTVRLFTPCTRHKQAPGDQVTGILPSVELLFNLDRITTVEHLLKSVLLYNINNSVSFSSAVKCVCNLMIKEPKSSSRTLGRAPYSFTFNSQFEFGKKHKWIQIDVTSLLQPLVASNKRSIHMSINFTCMKDQLEHPSAQNGLFNMTLVSPSLILYLNDTSAQAYHSWYSLHYKRRPSQGPDQERSLSAYPVGEEAAEDGRSSHHRHRRGQETVSSELKKPLGPASFNLSEYFRQFLLPQNECELHDFRLSFSQLKWDNWIVAPHRYNPRYCKGDCPRAVGHRYGSPVHTMVQNIIYEKLDSSVPRPSCVPAKYSPLSVLTIEPDGSIAYKEYEDMIATKCTCR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O60383 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S156 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| T189 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| Y394 | Phosphorylation | Uniprot | |
| Y407 | Phosphorylation | Uniprot | |
| Y440 | Phosphorylation | Uniprot |
研究背景
Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.
Phosphorylated; phosphorylation is critical for GDF9 function. In vitro, can be phosphorylated by CK at Ser-325.
Secreted.
Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level).
Homodimer or heterodimer (Potential). But, in contrast to other members of this family, cannot be disulfide-linked (By similarity).
Belongs to the TGF-beta family.
研究領域
· Organismal Systems > Endocrine system > Ovarian steroidogenesis.
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