Phospho-SKI (Ser383) Antibody - #AF7036
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7036, RRID:AB_2843476.
折りたたみ／展開
Avian sarcoma viral (v ski) oncogene homolog; C oncogene; C ski; Proto-oncogene c-Ski; SKI; Ski oncogene; Ski oncoprotein; SKI_HUMAN; SKV; v ski avian sarcoma viral oncogene homolog; v ski sarcoma viral oncogene homolog;
免疫原
- P12755 SKI_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAAAGGRGCFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQEAYKKESAKEAGAAAVPAPVPAATEPPPVLHLPAIQPPPPVLPGPFFMPSDRSTERCETVLEGETISCFVVGGEKRLCLPQILNSVLRDFSLQQINAVCDELHIYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYGGAYPPPCKKELAASLALGLELSERSVRVYHECFGKCKGLLVPELYSSPSAACIQCLDCRLMYPPHKFVVHSHKALENRTCHWGFDSANWRAYILLSQDYTGKEEQARLGRCLDDVKEKFDYGNKYKRRVPRVSSEPPASIRPKTDDTSSQSPAPSEKDKPSSWLRTLAGSSNKSLGCVHPRQRLSAFRPWSPAVSASEKELSPHLPALIRDSFYSYKSFETAVAPNVALAPPAQQKVVSSPPCAAAVSRAPEPLATCTQPRKRKLTVDTPGAPETLAPVAAPEEDKDSEAEVEVESREEFTSSLSSLSSPSFTSSSSAKDLGSPGARALPSAVPDAAAPADAPSGLEAELEHLRQALEGGLDTKEAKEKFLHEVVKMRVKQEEKLSAALQAKRSLHQELEFLRVAKKEKLREATEAKRNLRKEIERLRAENEKKMKEANESRLRLKRELEQARQARVCDKGCEAGRLRAKYSAQIEDLQVKLQHAEADREQLRADLLREREAREHLEKVVKELQEQLWPRARPEAAGSEGAAELEP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P12755 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T103 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| K318 | Acetylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | O14965 (AURKA) | Uniprot |
| K335 | Acetylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | O14965 (AURKA) | Uniprot |
| S394 | Phosphorylation | Uniprot | |
| S404 | Phosphorylation | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| T458 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S480 | Phosphorylation | Uniprot | |
| S500 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| K584 | Ubiquitination | Uniprot | |
| K662 | Ubiquitination | Uniprot | |
| K673 | Ubiquitination | Uniprot | |
| S720 | Phosphorylation | Uniprot |
研究背景
May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
Ubiquitinated by RNF165, promoting proteasomal degradation, leading to enhance the BMP-Smad signaling.
Nucleus.
Interacts with SMAD2, SMAD3 and SMAD4. Interacts with HIPK2. Part of a complex with HIPK2 and SMAD1/2/3. Interacts with PRDM16 and SMAD3; the interaction with PRDM16 promotes the recruitment SMAD3-HDAC1 complex on the promoter of TGF-beta target genes.
Belongs to the SKI family.
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