製品: Phospho-EIF4A1 (Tyr48) Antibody
カタログ: AF7054
タンパク質の説明: Rabbit polyclonal antibody to Phospho-EIF4A1 (Tyr48)
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 46kDa; 46kD(Calculated).
ユニプロット: P60842
RRID: AB_2843494

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-EIF4A1 (Tyr48) Antibody detects endogenous levels of EIF4A1 only when phosphorylated at Tyr48.
RRID:
AB_2843494
引用形式: Affinity Biosciences Cat# AF7054, RRID:AB_2843494.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ATP-dependent RNA helicase eIF4A-1; DDX2; DDX2A; eIF 4A I; eIF-4A-I; EIF4A; eIF4A I; eIF4A-I; EIF4A1; Eukaryotic initiation factor 4A-I; Eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A, isoform 1; Eukaryotic translation initiation factor 4A1; IF4A1_HUMAN;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P60842 基板として

Site PTM Type Enzyme
S2 Acetylation
S4 Phosphorylation
Y48 Phosphorylation
Y50 Phosphorylation
K54 Acetylation
K54 Methylation
K54 Sumoylation
K54 Ubiquitination
C66 S-Nitrosylation
K68 Sumoylation
K68 Ubiquitination
Y70 Phosphorylation
S78 Phosphorylation
T80 Phosphorylation
K82 Ubiquitination
K99 Sumoylation
K118 Acetylation
K118 Ubiquitination
S130 Phosphorylation
K146 Acetylation
K146 Sumoylation
K146 Ubiquitination
T158 Phosphorylation
R168 Methylation
K174 Acetylation
K174 Ubiquitination
K177 Ubiquitination
S189 Phosphorylation
K193 Acetylation
K193 Methylation
K193 Sumoylation
K193 Ubiquitination
Y197 Phosphorylation
K202 Sumoylation
K202 Ubiquitination
S213 Phosphorylation
K225 Sumoylation
K225 Ubiquitination
K226 Ubiquitination
K237 Ubiquitination
K238 Acetylation
K238 Sumoylation
K238 Ubiquitination
T242 Phosphorylation
Y250 Phosphorylation
R255 Methylation
K284 Ubiquitination
K291 Acetylation
K291 Sumoylation
K291 Ubiquitination
K309 Acetylation
K309 Ubiquitination
R316 Methylation
R353 Methylation
K369 Ubiquitination
T377 Phosphorylation
K381 Acetylation
K381 Ubiquitination

研究背景

機能:

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

サブユニット構造:

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4. Interacts with DDX3X in an RNA-independent manner.

(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL69.

タンパク質ファミリー:

Belongs to the DEAD box helicase family. eIF4A subfamily.

研究領域

· Genetic Information Processing > Translation > RNA transport.

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