Phospho-EED (Thr55) Antibody - #AF7058
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7058, RRID:AB_2843498.
折りたたみ／展開
eed; EED protein; EED, mouse, homolog of; EED_HUMAN; Embryonic ectoderm development; Embryonic ectoderm development isoform a; Embryonic ECTODERM development protein homolog; Embryonic ectoderm development protein, mouse, homolog of; hEED; OTTHUMP00000235483; OTTHUMP00000235484; OTTHUMP00000235485; Polycomb protein eed; WAIT 1; WAIT-1; WAIT1; WD protein associating with integrin cytoplasmic tails 1;
免疫原
Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
- O75530 EED_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O75530 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| K19 | Acetylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| T50 | Phosphorylation | Uniprot | |
| T55 | Phosphorylation | Uniprot | |
| T57 | Phosphorylation | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| K66 | Methylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| T115 | Phosphorylation | Uniprot | |
| K197 | Methylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K268 | Methylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| Y278 | Phosphorylation | Uniprot | |
| Y280 | Phosphorylation | Uniprot | |
| K284 | Methylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| K408 | Ubiquitination | Uniprot |
研究背景
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.
Nucleus. Chromosome.
Note: Transiently colocalizes with XIST at inactive X chromosomes.
Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC, HDAC2, histone H1 and YY1. May interact with ITGA4, ITGAE and ITGB7. Interacts with CDYL. Interacts with ARNTL/BMAL1. Interacts with KMT2A/MLL1 (By similarity).
(Microbial infection) May interact with the MA protein of HIV-1.
The WD repeat domain mediates recognition of trimethylated histone peptides at the consensus sequence Ala-Arg-Lys-Ser. This is achieved through an aromatic cage encircling the methyllysine, and involving Phe-97, Tyr-148 and Tyr-365.
Belongs to the WD repeat ESC family.
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