Phospho-CDC5L (Tyr232) Antibody - #AF7063
| 製品: | Phospho-CDC5L (Tyr232) Antibody |
| カタログ: | AF7063 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-CDC5L (Tyr232) |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 92kDa; 92kD(Calculated). |
| ユニプロット: | Q99459 |
| RRID: | AB_2843503 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7063, RRID:AB_2843503.
折りたたみ／展開
CDC5; CDC5 cell division cycle 5-like (S. pombe); Cdc5 related protein; CDC5-LIKE; Cdc5-like protein; Cdc5l; CDC5L_HUMAN; CEF1; Cell division cycle 5 S. pombe homolog of; Cell division cycle 5-like protein; dJ319D22.1; KIAA0432; PCDC5RP; Pombe Cdc5 related protein; Pombe Cdc5-related protein;
免疫原
- Q99459 CDC5L_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKKTEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDPRKLKPGEIDPNPETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIEIQKKRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFRKLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAPQISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGAEGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99459 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K25 | Ubiquitination | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K165 | Methylation | Uniprot | |
| K166 | Acetylation | Uniprot | |
| K168 | Acetylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| Y210 | Phosphorylation | Uniprot | |
| K219 | Sumoylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| Y225 | Phosphorylation | Uniprot | |
| T227 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| Y232 | Phosphorylation | Uniprot | |
| R252 | Methylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| T344 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| T355 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| T373 | Phosphorylation | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| K380 | Ubiquitination | Uniprot | |
| T385 | Phosphorylation | Uniprot | |
| S390 | Phosphorylation | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| T404 | Phosphorylation | Uniprot | |
| T407 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T411 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T415 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| T424 | Phosphorylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| T429 | Phosphorylation | Uniprot | |
| T430 | Phosphorylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| T438 | Phosphorylation | Uniprot | |
| T442 | Phosphorylation | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| Y459 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| Y464 | Phosphorylation | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| K487 | Ubiquitination | Uniprot | |
| K500 | Ubiquitination | Uniprot | |
| T510 | Phosphorylation | Uniprot | |
| Y511 | Phosphorylation | Uniprot | |
| Y621 | Phosphorylation | Uniprot | |
| K623 | Ubiquitination | Uniprot | |
| K626 | Acetylation | Uniprot | |
| S691 | Phosphorylation | Uniprot | |
| K694 | Ubiquitination | Uniprot | |
| Y788 | Phosphorylation | Uniprot | |
| K795 | Ubiquitination | Uniprot | |
| T797 | Phosphorylation | Uniprot |
研究背景
DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).
Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.
Nucleus. Nucleus speckle. Cytoplasm.
Note: May shuttle between cytoplasm and nucleus.
Ubiquitously expressed in both fetal and adult tissues.
Homodimer. Interacts with DAPK3 (By similarity). Component of the precatalytic, catalytic and postcatalytic spliceosome complexes. Part of a spliceosomal 'core' complex consisting of CDC5L, PLRG1, SPF27, CCAP1, CCAP3 and CCAP6. Interacts with PLRG1, Lodestar/TTF2, and NIPP1/PPP1R8. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its C-terminus) directly in the complex with PRPF19 and BCAS2. Interacts (via its C-terminus) directly with PRGL1 (via its WD40 repeat domain); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts with CTNNBL1. Interacts with PRPF19 (via N-terminus) (By similarity).
Belongs to the CEF1 family.
研究領域
· Genetic Information Processing > Transcription > Spliceosome.
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