Phospho-Occludin (Tyr287) Antibody - #AF7069

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製品: Phospho-Occludin (Tyr287) Antibody
カタログ: AF7069
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Occludin (Tyr287)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 23~30kD, 52~65kD; 59kD(Calculated).
ユニプロット: Q16625
RRID: AB_2843509

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-Occludin (Tyr287) Antibody detects endogenous levels of Occludin only when phosphorylated at Tyr287.
RRID:
AB_2843509
引用形式: Affinity Biosciences Cat# AF7069, RRID:AB_2843509.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; Ocln; OCLN_HUMAN; Tight junction protein occludin;

免疫原

免疫原:
Uniprot:

Q16625(OCLN_HUMAN) >>Visit HPA database.

遺伝子(ID):
OCLN(100506658)
発現特異性:
Q16625 OCLN_HUMAN:

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

タンパク質配列:
MSSRPLESPPPYRPDEFKPNHYAPSNDIYGGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLIIVMCIAIFACVASTLAWDRGYGTSLLGGSVGYPYGGSGFGSYGSGYGYGYGYGYGYGGYTDPRAAKGFMLAMAAFCFIAALVIFVTSVIRSEMSRTRRYYLSVIIVSAILGIMVFIATIVYIMGVNPTAQSSGSLYGSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMIIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
89
Horse
89
Bovine
89
Sheep
89
Rabbit
78
Xenopus
57
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16625 基板として

Site PTM Type Enzyme
S8 Phosphorylation
K18 Ubiquitination
S40 Phosphorylation
Y44 Phosphorylation
S45 Phosphorylation
K276 Ubiquitination
S277 Phosphorylation
K283 Ubiquitination
Y287 Phosphorylation
K299 Ubiquitination
S302 Phosphorylation
T305 Phosphorylation
S310 Phosphorylation
S313 Phosphorylation
Y315 Phosphorylation
S321 Phosphorylation
Y325 Phosphorylation
S326 Phosphorylation
S327 Phosphorylation
K330 Ubiquitination
Y337 Phosphorylation
S340 Phosphorylation
S341 Phosphorylation
Y342 Phosphorylation
K343 Ubiquitination
S344 Phosphorylation
T345 Phosphorylation
S358 Phosphorylation
Y368 Phosphorylation
S369 Phosphorylation
S370 Phosphorylation
T376 Phosphorylation
S378 Phosphorylation
K379 Ubiquitination
T393 Phosphorylation
Y398 Phosphorylation P12931 (SRC)
T400 Phosphorylation P68400 (CSNK2A1)
Y402 Phosphorylation P12931 (SRC)
T403 Phosphorylation Q02156 (PRKCE) , P24723 (PRKCH) , Q05513 (PRKCZ)
T404 Phosphorylation Q02156 (PRKCE) , Q05513 (PRKCZ) , P24723 (PRKCH) , P68400 (CSNK2A1) , P67870 (CSNK2B)
S408 Phosphorylation P67870 (CSNK2B) , P68400 (CSNK2A1)
T424 Phosphorylation Q02156 (PRKCE) , Q05513 (PRKCZ)
T438 Phosphorylation Q02156 (PRKCE) , Q05513 (PRKCZ)
Y443 Phosphorylation
S445 Phosphorylation
S448 Phosphorylation
S458 Phosphorylation
Y467 Phosphorylation
S471 Phosphorylation
Y474 Phosphorylation
Y481 Phosphorylation
K488 Ubiquitination
S490 Phosphorylation P05771 (PRKCB)

研究背景

機能:

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.

PTMs:

Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Cell junction>Tight junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

サブユニット構造:

Interacts with TJP1/ZO1. Interacts with VAPA. Interacts with CLDN1, CLDN6, CLDN9, CLDN11, CLDN12 and CLDN17.

タンパク質ファミリー:

The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.

Belongs to the ELL/occludin family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

参考文献

1). Atorvastatin-induced intracerebral hemorrhage is inhibited by berberine in zebrafish. Journal of applied toxicology : JAT, 2024 (PubMed: 38639436) [IF=3.3]
2). Protective effects of lycium barbarum polysaccharides on blood-retinal barrier via ROCK1 pathway in diabetic rats. American Journal of Translational Research, 2023 (PubMed: 31737184) [IF=2.2]

Application: WB    Species: rat    Sample:

Figure 7. | Western blot assay for P-Occludin, ROCK1 and P-MLC protein expression. The Western blot was performed at 4, 8 and 12 weeks after administration. The relative expression of P-Occludin, ROCK1 and P-MLC was displayed in (A-C) respectively. (D) Western blot assay. *P<0.05, **P<0.01 vs. CON group; #P<0.05, ##P<0.01 vs. 8 w DM group.

Application: IHC    Species: rat    Sample:

Figure 6.| Immunohistochemistry (IHC) assay for P-Occludin and ROCK1 protein expression. The IHC assay was performed at 4, 8 and 12 weeks after administration. CON: control group treated with normal saline; DM: model group treated with normal saline; LBP: model group treated with LBP; F: model group treated with fasudil; F + LBP: model group treated with fasudil and LBP.

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