Phospho-CUL4B (Thr48) Antibody - #AF7083
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7083, RRID:AB_2843523.
折りたたみ／展開
CUL 4B; CUL-4B; CUL4B; CUL4B_HUMAN; Cullin-4B; DKFZp686F1470; KIAA0695; MRXHF2; MRXSC; SFM2;
免疫原
- Q13620 CUL4B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSSTTVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQNKTIDGILLLIERERNGEAIDRSLLRSLLSMLSDLQIYQDSFEQRFLEETNRLYAAEGQKLMQEREVPEYLHHVNKRLEEEADRLITYLDQTTQKSLIATVEKQLLGEHLTAILQKGLNNLLDENRIQDLSLLYQLFSRVRGGVQVLLQQWIEYIKAFGSTIVINPEKDKTMVQELLDFKDKVDHIIDICFLKNEKFINAMKEAFETFINKRPNKPAELIAKYVDSKLRAGNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMIQFKQYMQNQNVPGNIELTVNILTMGYWPTYVPMEVHLPPEMVKLQEIFKTFYLGKHSGRKLQWQSTLGHCVLKAEFKEGKKELQVSLFQTLVLLMFNEGEEFSLEEIKQATGIEDGELRRTLQSLACGKARVLAKNPKGKDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTERVFQDRQYQIDAAIVRIMKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13620 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T46 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| T49 | Phosphorylation | Uniprot | |
| T52 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| T85 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| T148 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| K158 | Methylation | Uniprot | |
| K160 | Methylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| K190 | Acetylation | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K258 | Methylation | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| Y413 | Phosphorylation | Uniprot | |
| K419 | Ubiquitination | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K539 | Ubiquitination | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| K565 | Ubiquitination | Uniprot | |
| K570 | Ubiquitination | Uniprot | |
| K585 | Ubiquitination | Uniprot | |
| Y598 | Phosphorylation | Uniprot | |
| Y607 | Phosphorylation | Uniprot | |
| K608 | Ubiquitination | Uniprot | |
| K619 | Ubiquitination | Uniprot | |
| K634 | Ubiquitination | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| K650 | Ubiquitination | Uniprot | |
| R779 | Methylation | Uniprot | |
| C787 | S-Nitrosylation | Uniprot | |
| K789 | Ubiquitination | Uniprot | |
| K800 | Acetylation | Uniprot | |
| K807 | Ubiquitination | Uniprot | |
| K815 | Methylation | Uniprot | |
| K815 | Ubiquitination | Uniprot | |
| K829 | Methylation | Uniprot | |
| R846 | Methylation | Uniprot | |
| Y874 | Phosphorylation | Uniprot | |
| K882 | Ubiquitination | Uniprot | |
| S892 | Phosphorylation | Uniprot | |
| R896 | Methylation | Uniprot | |
| Y898 | Phosphorylation | Uniprot | |
| K903 | Ubiquitination | Uniprot |
研究背景
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8. With CUL4A, contributes to ribosome biogenesis.
Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
Nucleus.
Component of multiple DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes that seem to be formed of DDB1, CUL4A or CUL4B, RBX1 and a variable substrate recognition component which seems to belong to a protein family described as DCAF (Ddb1- and Cul4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Component of the DCX(DTL) complex with the putative substrate recognition component DTL. Component of the DCX(DDB2) complex with the putative substrate recognition component DDB2. Part of a complex with RBX1 and TIP120A/CAND1. Interacts with RBX1 GRWD1, MLST8, SMU1, TLE2, TLE3, DCAF1, DDA1, DCAF6, DCAF17, DDB2, DCAF8, TIP120A/CAND1 and TMEM113. Interacts with cyclin E and with importins alpha-1 (KPNA2), alpha-3 (KPNA4), alpha-5 (KPNA1) and beta-1 (KPNB1). May interact with WDR26, WDR51B, SNRNP40, WDR61, WDR76 and WDR5.
Belongs to the cullin family.
研究領域
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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