Phospho-LPIN1 (Thr14) Antibody - #AF7085
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| 製品: | Phospho-LPIN1 (Thr14) Antibody |
| カタログ: | AF7085 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-LPIN1 (Thr14) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 99kDa; 99kD(Calculated). |
| ユニプロット: | Q14693 |
| RRID: | AB_2843525 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7085, RRID:AB_2843525.
折りたたみ／展開
EC=3.1.3.4; KIAA0188; Lipin-1; Lpin1; LPIN1_HUMAN; PAP1; Phosphatidate phosphatase LPIN1;
免疫原
Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract.
- Q14693 LPIN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVIRQPNGNLQCSPFHVRFGKMGVLRSREKVVDIEINGESVDLHMKLGDNGEAFFVQETDNDQEVIPMHLATSPILSEGASRMECQLKRGSVDRMRGLDPSTPAQVIAPSETPSSSSVVKKRRKRRRKSQLDSLKRDDNMNTSEDEDMFPIEMSSDEAMELLESSRTLPNDIPPFQDDIPEENLSLAVIYPQSASYPNSDREWSPTPSPSGSRPSTPKSDSELVSKSTERTGQKNPEMLWLWGELPQAAKSSSPHKMKESSPLSSRKICDKSHFQAIHSESSDTFSDQSPTLVGGALLDQNKPQTEMQFVNEEDLETLGAAAPLLPMIEELKPPSASVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDPSGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRDLPSIAISLCGGLSDHREITKDAFLEQAVSYQQFVDNPAIIDDPNLVVKIGSKYYNWTTAAPLLLAMQAFQKPLPKATVESIMRDKMPKKGGRWWFSWRGRNTTIKEESKPEQCLAGKAHSTGEQPPQLSLATRVKHESSSSDEERAAAKPSNAGHLPLLPNVSYKKTLRLTSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITRSDTLGHILPTLGKDWTHQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSALHREVIEKKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFTVNPKGELVQEHAKTNISSYVRLCEVVDHVFPLLKRSHSSDFPCSDTFSNFTFWREPLPPFENQDIHSASA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14693 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T14 | Phosphorylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| S106 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S134 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| T145 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| T249 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S449 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| T563 | Phosphorylation | Uniprot | |
| S580 | Phosphorylation | Uniprot | |
| K609 | Ubiquitination | Uniprot | |
| K636 | Ubiquitination | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| K707 | Ubiquitination | Uniprot | |
| K711 | Ubiquitination | Uniprot | |
| S713 | Phosphorylation | Uniprot | |
| Y717 | Phosphorylation | Uniprot | |
| K770 | Ubiquitination | Uniprot | |
| K776 | Ubiquitination | Uniprot | |
| K854 | Ubiquitination | Uniprot | |
| S887 | Phosphorylation | Uniprot | |
| S889 | Phosphorylation | Uniprot |
研究背景
Plays important roles in controlling the metabolism of fatty acids at different levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol (By similarity).
Phosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway. Phosphorylation is decreased by epinephrine. Phosphorylation may not directly affect the catalytic activity but may regulate the localization. Dephosphorylated by the CTDNEP1-CNEP1R1 complex (By similarity).
Sumoylated.
Nucleus membrane. Cytoplasm>Cytosol. Endoplasmic reticulum membrane.
Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract.
Interacts (via LXXIL motif) with PPARA. Interacts with PPARGC1A. Interaction with PPARA and PPARGC1A leads to the formation of a complex that modulates gene transcription. Interacts with MEF2C.
Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.
Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.
Belongs to the lipin family.
研究領域
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Metabolism > Lipid metabolism > Glycerolipid metabolism.
· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
参考文献
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