Phospho-FANCA (Ser1449) Antibody - #AF7113
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7113, RRID:AB_2843553.
折りたたみ／展開
FA 1; FA; FA H; FA1; FAA; FACA; FAH; Fanca; FANCA_HUMAN; FANCH; Fanconi anemia complementation group A; Fanconi anemia complementation group H; Fanconi anemia group A protein; Fanconi anemia type 1; MGC75158; Protein FACA;
免疫原
- O15360 FANCA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDSWVPNSASGQDPGGRRRAWAELLAGRVKREKYNPERAQKLKESAVRLLRSHQDLNALLLEVEGPLCKKLSLSKVIDCDSSEAYANHSSSFIGSALQDQASRLGVPVGILSAGMVASSVGQICTAPAETSHPVLLTVEQRKKLSSLLEFAQYLLAHSMFSRLSFCQELWKIQSSLLLEAVWHLHVQGIVSLQELLESHPDMHAVGSWLFRNLCCLCEQMEASCQHADVARAMLSDFVQMFVLRGFQKNSDLRRTVEPEKMPQVTVDVLQRMLIFALDALAAGVQEESSTHKIVRCWFGVFSGHTLGSVISTDPLKRFFSHTLTQILTHSPVLKASDAVQMQREWSFARTHPLLTSLYRRLFVMLSAEELVGHLQEVLETQEVHWQRVLSFVSALVVCFPEAQQLLEDWVARLMAQAFESCQLDSMVTAFLVVRQAALEGPSAFLSYADWFKASFGSTRGYHGCSKKALVFLFTFLSELVPFESPRYLQVHILHPPLVPGKYRSLLTDYISLAKTRLADLKVSIENMGLYEDLSSAGDITEPHSQALQDVEKAIMVFEHTGNIPVTVMEASIFRRPYYVSHFLPALLTPRVLPKVPDSRVAFIESLKRADKIPPSLYSTYCQACSAAEEKPEDAALGVRAEPNSAEEPLGQLTAALGELRASMTDPSQRDVISAQVAVISERLRAVLGHNEDDSSVEISKIQLSINTPRLEPREHMAVDLLLTSFCQNLMAASSVAPPERQGPWAALFVRTMCGRVLPAVLTRLCQLLRHQGPSLSAPHVLGLAALAVHLGESRSALPEVDVGPPAPGAGLPVPALFDSLLTCRTRDSLFFCLKFCTAAISYSLCKFSSQSRDTLCSCLSPGLIKKFQFLMFRLFSEARQPLSEEDVASLSWRPLHLPSADWQRAALSLWTHRTFREVLKEEDVHLTYQDWLHLELEIQPEADALSDTERQDFHQWAIHEHFLPESSASGGCDGDLQAACTILVNALMDFHQSSRSYDHSENSDLVFGGRTGNEDIISRLQEMVADLELQQDLIVPLGHTPSQEHFLFEIFRRRLQALTSGWSVAASLQRQRELLMYKRILLRLPSSVLCGSSFQAEQPITARCEQFFHLVNSEMRNFCSHGGALTQDITAHFFRGLLNACLRSRDPSLMVDFILAKCQTKCPLILTSALVWWPSLEPVLLCRWRRHCQSPLPRELQKLQEGRQFASDFLSPEAASPAPNPDWLSAAALHFAIQQVREENIRKQLKKLDCEREELLVFLFFFSLMGLLSSHLTSNSTTDLPKAFHVCAAILECLEKRKISWLALFQLTESDLRLGRLLLRVAPDQHTRLLPFAFYSLLSYFHEDAAIREEAFLHVAVDMYLKLVQLFVAGDTSTVSPPAGRSLELKGQGNPVELITKARLFLLQLIPRCPKKSFSHVAELLADRGDCDPEVSAALQSRQQAAPDADLSQEPHLF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O15360 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K34 | Ubiquitination | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| T256 | Phosphorylation | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| T329 | Phosphorylation | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| T351 | Phosphorylation | P51955 (NEK2) | Uniprot |
| S357 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| Y488 | Phosphorylation | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| K608 | Ubiquitination | Uniprot | |
| K612 | Ubiquitination | Uniprot | |
| K631 | Ubiquitination | Uniprot | |
| R683 | Methylation | Uniprot | |
| K701 | Ubiquitination | Uniprot | |
| S849 | Phosphorylation | Uniprot | |
| S850 | Phosphorylation | Uniprot | |
| S858 | Phosphorylation | Uniprot | |
| K921 | Sumoylation | Uniprot | |
| K1079 | Ubiquitination | Uniprot | |
| S1149 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K1199 | Ubiquitination | Uniprot | |
| S1208 | Phosphorylation | Uniprot | |
| S1301 | Phosphorylation | Uniprot | |
| T1309 | Phosphorylation | Uniprot | |
| K1387 | Ubiquitination | Uniprot | |
| K1398 | Ubiquitination | Uniprot | |
| K1413 | Ubiquitination | Uniprot | |
| S1449 | Phosphorylation | Q13315 (ATM) , Q13535 (ATR) | Uniprot |
研究背景
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H.
Nucleus. Cytoplasm.
Note: The major form is nuclear. The minor form is cytoplasmic.
Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. In complex with FANCF, FANCG and FANCL, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins. The complex with FANCC and FANCG may also include EIF2AK2 and HSP70. Interacts with FAAP20/C1orf86; interaction is direct.
研究領域
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
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