Phospho-AS160 (Ser341) Antibody - #AF7130
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7130, RRID:AB_2843570.
折りたたみ／展開
Acrg embryonic lethality (mouse) minimal region ortholog; Acrg embryonic lethality minimal region ortholog; Acrg embryonic lethality mouse minimal region ortholog; Akt substrate of 160 kDa; AS 160; AS160; BUB2; CDC16; KIAA0603; NIDDM5; TBC (Tre 2 BUB2 CDC16) domain containing protein; TBC Tre 2 BUB2 CDC16 domain containing protein; TBC1 D4; TBC1 domain family member 4; Tbc1d4; TBCD4_HUMAN; Tre-2;
免疫原
Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.
- O60343 TBCD4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISSCSQGIKHVDHFGFICRESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPSTISNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSNLSSVRRMYKESNSSSSLPSLHTSFSAPSFTAPSFLKSFYQNSGRLSPQYENEIRQDTASESSDGEGRKRTSSTCSNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLPLSPLSPTMEEEPLVVFLSGEDDPEKIEERKKSKELRSLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLKQLTAQQHAILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFSLGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFENIVEFLKNTLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQESSYSCEDSETLEKLERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLLTRETKMKSLIRTLEQEKMAYQKTVEQLRKLLPADALVNCDLLLRDLNCNPNNKAKIGNKP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O60343 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | O00141 (SGK1) , P31749 (AKT1) | Uniprot |
| T320 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Q15418 (RPS6KA1) | Uniprot |
| S344 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| T356 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| K477 | Acetylation | Uniprot | |
| S485 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S486 | Phosphorylation | Uniprot | |
| T488 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| K560 | Ubiquitination | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| T568 | Phosphorylation | O00141 (SGK1) | Uniprot |
| S569 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | O14757 (CHEK1) , P31749 (AKT1) , O00141 (SGK1) | Uniprot |
| S591 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S609 | Phosphorylation | Uniprot | |
| T613 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| S633 | Phosphorylation | Uniprot | |
| T642 | Phosphorylation | O00141 (SGK1) , P31749 (AKT1) | Uniprot |
| S644 | Phosphorylation | Uniprot | |
| S648 | Phosphorylation | Uniprot | |
| S649 | Phosphorylation | Uniprot | |
| S666 | Phosphorylation | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| S673 | Phosphorylation | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| T701 | Phosphorylation | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| S707 | Phosphorylation | Uniprot | |
| T749 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | O00141 (SGK1) , Q15418 (RPS6KA1) | Uniprot |
| T752 | Phosphorylation | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| S757 | Phosphorylation | Uniprot | |
| S759 | Phosphorylation | Uniprot | |
| T763 | Phosphorylation | Uniprot | |
| S764 | Phosphorylation | Uniprot | |
| T766 | Phosphorylation | Uniprot | |
| S782 | Phosphorylation | Uniprot | |
| S787 | Phosphorylation | Uniprot | |
| S789 | Phosphorylation | Uniprot | |
| S806 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| Y1191 | Phosphorylation | Uniprot | |
| S1207 | Phosphorylation | Uniprot | |
| S1246 | Phosphorylation | Uniprot | |
| K1255 | Ubiquitination | Uniprot | |
| K1267 | Ubiquitination | Uniprot |
研究背景
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Phosphorylated by AKT1; insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
Cytoplasm.
Note: Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.
研究領域
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
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