Phospho-STAM2 (Tyr371) Antibody - #AF7158
| 製品: | Phospho-STAM2 (Tyr371) Antibody |
| カタログ: | AF7158 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-STAM2 (Tyr371) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 58kDa; 58kD(Calculated). |
| ユニプロット: | O75886 |
| RRID: | AB_2843598 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7158, RRID:AB_2843598.
折りたたみ／展開
DKFZp564C047; Hbp; Hrs-binding protein; HSE1 homolog; Signal transducing adapter molecule 2; Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2; Signal transducing adaptor molecule 2A; Signal transducing adaptor molecule 2B; Signal transducing adaptor molecule; STAM like protein containing SH3 and ITAM domains 2; STAM-2; Stam2; STAM2_HUMAN; STAM2A; STAM2B;
免疫原
- O75886 STAM2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPLFTANPFEQDVEKATNEYNTTEDWSLIMDICDKVGSTPNGAKDCLKAIMKRVNHKVPHVALQALTLLGACVANCGKIFHLEVCSRDFATEVRAVIKNKAHPKVCEKLKSLMVEWSEEFQKDPQFSLISATIKSMKEEGITFPPAGSQTVSAAAKNGTSSNKNKEDEDIAKAIELSLQEQKQQHTETKSLYPSSEIQLNNKVARKVRALYDFEAVEDNELTFKHGEIIIVLDDSDANWWKGENHRGIGLFPSNFVTTNLNIETEAAAVDKLNVIDDDVEEIKKSEPEPVYIDEDKMDRALQVLQSIDPTDSKPDSQDLLDLEDICQQMGPMIDEKLEEIDRKHSELSELNVKVLEALELYNKLVNEAPVYSVYSKLHPPAHYPPASSGVPMQTYPVQSHGGNYMGQSIHQVTVAQSYSLGPDQIGPLRSLPPNVNSSVTAQPAQTSYLSTGQDTVSNPTYMNQNSNLQSATGTTAYTQQMGMSVDMSSYQNTTSNLPQLAGFPVTVPAHPVAQQHTNYHQQPLL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O75886 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| K57 | Ubiquitination | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| T159 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| Y291 | Phosphorylation | P06241 (FYN) | Uniprot |
| K296 | Ubiquitination | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| Y371 | Phosphorylation | P06241 (FYN) | Uniprot |
| S372 | Phosphorylation | Uniprot | |
| Y374 | Phosphorylation | P06241 (FYN) | Uniprot |
| S375 | Phosphorylation | Uniprot | |
| Y418 | Phosphorylation | Uniprot |
研究背景
Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).
Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF.
Cytoplasm. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side.
Ubiquitously expressed.
Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 and EPS15. Interacts with JAK2 and JAK3. Interacts with ubiquitinated proteins and the deubiquitinating enzyme USP8/UBPY (By similarity). Interacts (via the via the PxVxL motif) with CBX5; the interaction is direct. Interacts with VPS37C. Interacts with ubiquitin; the interaction is direct. Interacts (via UIM domain) with UBQLN1 (via ubiquitin-like domain) (By similarity).
The VHS and UIM domains mediate the interaction with ubiquitinated proteins.
The SH3 domain mediates the interaction with USP8.
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
Belongs to the STAM family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
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