Phospho-RAD17 (Ser86) Antibody - #AF7161
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7161, RRID:AB_2843601.
折りたたみ／展開
CCYC; Cell cycle checkpoint protein; Cell cycle checkpoint protein RAD17; FLJ41520; HRAD 17; hRad17; R24L; Rad 17; Rad 24; RAD1 (S. pombe) homolog; RAD1 homolog; RAD17; RAD17 homolog (S. pombe); RAD17 homolog; Rad17 like protein; RAD17, S. pombe, homolog of; RAD17_HUMAN; RAD17Sp; Rad24; Rad24, mouse, homolog of; Rad24, S. cerevisiae, homolog of; RF C activator 1 homolog; RF C/activator 1 homolog; RF-C/activator 1 homolog;
免疫原
Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
- O75943 RAD17_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSKTFLRPKVSSTKVTDWVDPSFDDFLECSGVSTITATSLGVNNSSHRRKNGPSTLESSRFPARKRGNLSSLEQIYGLENSKEYLSENEPWVDKYKPETQHELAVHKKKIEEVETWLKAQVLERQPKQGGSILLITGPPGCGKTTTLKILSKEHGIQVQEWINPVLPDFQKDDFKGMFNTESSFHMFPYQSQIAVFKEFLLRATKYNKLQMLGDDLRTDKKIILVEDLPNQFYRDSHTLHEVLRKYVRIGRCPLIFIISDSLSGDNNQRLLFPKEIQEECSISNISFNPVAPTIMMKFLNRIVTIEANKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSSKGENNLRPRKKGMSLKSDAVLSKSKRRKKPDRVFENQEVQAIGGKDVSLFLFRALGKILYCKRASLTELDSPRLPSHLSEYERDTLLVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDWNTRSLLREYSTSIATRGVMHSNKARGYAHCQGGGSSFRPLHKPQWFLINKKYRENCLAAKALFPDFCLPALCLQTQLLPYLALLTIPMRNQAQISFIQDIGRLPLKRHFGRLKMEALTDREHGMIDPDSGDEAQLNGGHSAEESLGEPTQATVPETWSLPLSQNSASELPASQPQPFSAQGDMEENIIIEDYESDGT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O75943 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T55 | Phosphorylation | Uniprot | |
| R66 | Methylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| K597 | Ubiquitination | Uniprot | |
| T633 | Phosphorylation | Uniprot | |
| S646 | Phosphorylation | Q13535 (ATR) , Q13315 (ATM) | Uniprot |
| S656 | Phosphorylation | Q13315 (ATM) , Q13535 (ATR) | Uniprot |
| S678 | Phosphorylation | Uniprot |
研究背景
Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
Nucleus.
Note: Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
Part of a DNA-binding complex containing RFC2, RFC3, RFC4 and RFC5. Interacts with RAD1 and RAD9 within the RAD1-RAD9-HUS1 complex. Interacts with RAD9B, POLE, SNU13 and MCM7. DNA damage promotes interaction with ATR or ATM and disrupts interaction with the RAD1-RAD9-HUS1 complex.
Belongs to the rad17/RAD24 family.
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