Phospho-CRYAB (Ser19) Antibody - #AF7176
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7176, RRID:AB_2843616.
折りたたみ／展開
AACRYA; Alpha B crystallin; Alpha crystallin B chain; Alpha(B)-crystallin; Alpha-crystallin B chain; CRYA2; Cryab; CRYAB_HUMAN; Crystallin alpha B; Crystallin alpha polypeptide 2; CTPP2; Heat shock 20 kD like protein; Heat shock protein beta 5; Heat shock protein beta-5; HspB5; Renal carcinoma antigen NY REN 27; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component;
免疫原
Lens as well as other tissues (PubMed:838078, PubMed:2387586). Expressed in myocardial tissue (PubMed:28493373).
- P02511 CRYAB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDIAIHHPWIRRPFFPFHSPSRLFDQFFGEHLLESDLFPTSTSLSPFYLRPPSFLRAPSWFDTGLSEMRLEKDRFSVNLDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVLTVNGPRKQVSGPERTIPITREEKPAVTAAPKK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P02511 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| R22 | Methylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| R50 | Methylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S59 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| R74 | Methylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| K90 | Acetylation | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K92 | Acetylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| T132 | Phosphorylation | Uniprot | |
| T134 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| T144 | Phosphorylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| K166 | Acetylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| T170 | O-Glycosylation | Uniprot |
研究背景
May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.
Cytoplasm. Nucleus.
Note: Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (PubMed:19464326). Localizes at the Z-bands and the intercalated disk in cardiomyocytes (PubMed:28493373).
Lens as well as other tissues. Expressed in myocardial tissue.
Heteropolymer composed of three CRYAA and one CRYAB subunits. Aggregates with homologous proteins, including the small heat shock protein HSPB1, to form large heteromeric complexes. Inter-subunit bridging via zinc ions enhances stability, which is crucial as there is no protein turn over in the lens. Interacts with HSPBAP1 and TTN/titin. Interacts with TMEM109. Interacts with DES; binds rapidly during early stages of DES filament assembly and a reduced binding seen in the later stages.
Belongs to the small heat shock protein (HSP20) family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)
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