Phospho-eIF2 alpha (Tyr150) Antibody - #AF7188

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製品: Phospho-eIF2 alpha (Tyr150) Antibody
カタログ: AF7188
タンパク質の説明: Rabbit polyclonal antibody to Phospho-eIF2 alpha (Tyr150)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 38kDa; 36kD(Calculated).
ユニプロット: P05198
RRID: AB_2843628

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MS Report
HPLC Report
MSDS
説明書
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(90%)
クローナリティ:
Polyclonal
特異性:
Phospho-eIF2 alpha (Tyr150) Antibody detects endogenous levels of eIF2 alpha only when phosphorylated at Tyr150.
RRID:
AB_2843628
引用形式: Affinity Biosciences Cat# AF7188, RRID:AB_2843628.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

EIF 2 alpha; EIF 2; EIF 2A; EIF 2alpha; eIF-2-alpha; eIF-2A; EIF-2alpha; EIF2 alpha; EIF2; EIF2A; EIF2S1; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa; Eukaryotic translation initiation factor 2 subunit 1 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; IF2A_HUMAN;

免疫原

免疫原:
Uniprot:

P05198(IF2A_HUMAN) >>Visit HPA database.

遺伝子(ID):
EIF2S1(1965)
タンパク質配列:
MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEVDGDDDAEEMEAKAED

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
90
Pig
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05198 基板として

Site PTM Type Enzyme
S5 Phosphorylation
K12 Ubiquitination
S26 Phosphorylation
S49 Phosphorylation Q9NZJ5 (EIF2AK3) , Q9BQI3 (EIF2AK1) , P19525 (EIF2AK2) , Q75MR0 (HRI)
S52 Phosphorylation B4DS64 (PRKRIR) , P51812 (RPS6KA3) , P19525 (EIF2AK2) , P28482 (MAPK1) , Q75MR0 (HRI) , Q9BQI3 (EIF2AK1) , Q9NZJ5 (EIF2AK3) , Q05655 (PRKCD) , Q16539 (MAPK14) , Q9P2K8 (EIF2AK4)
S58 Phosphorylation
K61 Ubiquitination
K80 Ubiquitination
Y82 Phosphorylation
S86 Phosphorylation
K87 Ubiquitination
S91 Phosphorylation
K97 Ubiquitination
K101 Acetylation
K101 Ubiquitination
K104 Ubiquitination
K106 Ubiquitination
S110 Phosphorylation
K123 Ubiquitination
R133 Methylation
K141 Acetylation
K141 Ubiquitination
K143 Acetylation
K143 Ubiquitination
Y147 Phosphorylation
Y150 Phosphorylation
K154 Methylation
K154 Ubiquitination
S158 Phosphorylation
T185 Phosphorylation
K190 Ubiquitination
Y200 Phosphorylation
Y202 Phosphorylation
K209 Ubiquitination
S219 Phosphorylation
K226 Ubiquitination
Y235 Phosphorylation
T245 Phosphorylation
K259 Ubiquitination
K265 Ubiquitination
R266 Methylation
K276 Ubiquitination
T279 Phosphorylation
T281 Phosphorylation
T284 Phosphorylation
K312 Ubiquitination

研究背景

機能:

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

PTMs:

Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation (By similarity).

細胞の位置付け:

Cytoplasm>Stress granule.
Note: Colocalizes with NANOS3 in the stress granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interaction with METAP2 protects EIF2S1 from inhibitory phosphorylation (By similarity). Interacts with ABCF1 isoform 2. Associates with ribosomes. Interacts with DDX3X in an RNA-independent manner.

タンパク質ファミリー:

Belongs to the eIF-2-alpha family.

研究領域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

参考文献

1). FGF21 attenuates high uric acid‑induced endoplasmic reticulum stress, inflammation and vascular endothelial cell dysfunction by activating Sirt1. Molecular Medicine Reports, 2022 (PubMed: 34850960) [IF=3.4]

Application: WB    Species: Human    Sample: HUVECs

Figure 1. High UA levels inhibits FGF21 and Sirt1 expression levels and promotes endoplasmic reticulum stress in HUVECs. (A) mRNA expression levels of FGF21 and Sirt1 were determined using reverse transcription-quantitative PCR. (B) Protein expression levels of FGF21 and Sirt1 were determined using western blotting. (C) Protein levels of ATF4, CHOP and p-eIF2A/eIF2A were determined using western blotting. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 mg/dl UA control group. FGF21, fibroblast growth factor 21; UA, uric acid; Sirt1, sirtuin 1; CHOP, eIF2A, eukaryotic initiation factor 2; ATF4, activating transcription factor 4; p-, phosphorylated.

Application: WB    Species: human    Sample: HUVECs

Figure 1.| High UA levels inhibits FGF21 and Sirt1 expression levels and promotes endoplasmic reticulum stress in HUVECs.(C) Protein levels of ATF4, CHOP and p‑eIF2A/eIF2A were determined using western blotting. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 mg/dl UA control group. FGF21, fibroblast growth factor 21; UA, uric acid; Sirt1,sirtuin 1; CHOP, eIF2A, eukaryotic initiation factor 2; ATF4, activating transcription factor 4; p‑, phosphorylated.

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