Phospho-PKC beta 1 (Thr642+Thr644) Antibody - #AF7191

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製品: Phospho-PKC beta 1 (Thr642+Thr644) Antibody
カタログ: AF7191
タンパク質の説明: Rabbit polyclonal antibody to Phospho-PKC beta 1 (Thr642+Thr644)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Bovine, Sheep, Rabbit
分子量: 77kDa; 77kD(Calculated).
ユニプロット: P05771
RRID: AB_2843631

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Sheep(100%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-PKC beta 1 (Thr642+Thr644) Antibody detects endogenous levels of PKC beta 1 only when phosphorylated at Thr642+Thr644.
RRID:
AB_2843631
引用形式: Affinity Biosciences Cat# AF7191, RRID:AB_2843631.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

KPCB_HUMAN; PKC beta; PKC-B; PKC-beta; PKCB; Prkcb; PRKCB I; PRKCB II; PRKCB1; PRKCB2; Protein kinase C beta 1; Protein kinase C beta 2; Protein kinase C beta; Protein kinase C beta type; protein kinase C, beta 1 polypeptide;

免疫原

免疫原:
Uniprot:

P05771(KPCB_HUMAN) >>Visit HPA database.

遺伝子(ID):
PRKCB(5579)
タンパク質配列:
MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDRDVLIVLVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKASVDGWFKLLSQEEGEYFNVPVPPEGSEANEELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Rabbit
100
Horse
40
Dog
40
Xenopus
40
Chicken
40
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05771 基板として

Site PTM Type Enzyme
A2 Acetylation
S11 Phosphorylation
T17 Phosphorylation
K23 Ubiquitination
T40 Phosphorylation
T48 Phosphorylation
S51 Phosphorylation
K76 Ubiquitination
K91 Ubiquitination
Y123 Phosphorylation
T134 Phosphorylation
K141 Ubiquitination
S149 Phosphorylation
Y195 Phosphorylation
K197 Acetylation
K197 Ubiquitination
K199 Ubiquitination
S206 Phosphorylation
K216 Ubiquitination
T226 Phosphorylation
K232 Ubiquitination
S241 Phosphorylation
S311 Phosphorylation
T314 Phosphorylation
K315 Ubiquitination
K327 Ubiquitination
K350 Ubiquitination
S352 Phosphorylation
K362 Ubiquitination
T364 Phosphorylation
Y368 Phosphorylation
K391 Ubiquitination
K468 Ubiquitination
S476 Phosphorylation
K481 Ubiquitination
K489 Ubiquitination
T497 Phosphorylation
T498 Phosphorylation
K499 Ubiquitination
T500 Phosphorylation P53004 (BLVRA) , O15530 (PDPK1)
T504 Phosphorylation
Y507 Phosphorylation
Y515 Phosphorylation
K566 Ubiquitination
K595 Ubiquitination
T642 Phosphorylation P05771 (PRKCB)
T644 Phosphorylation
S661 Phosphorylation P05771 (PRKCB)
Y662 Phosphorylation P43405 (SYK)

PTMs - P05771 酵素として

Substrate Site Source
O15350 (TP73) S388 Uniprot
O15519 (CFLAR) S193 Uniprot
O95786 (DDX58) S8 Uniprot
O95786 (DDX58) T170 Uniprot
P04004 (VTN) S381 Uniprot
P04049 (RAF1) S497 Uniprot
P04049 (RAF1) S619 Uniprot
P04083 (ANXA1) S27 Uniprot
P04083 (ANXA1) S28 Uniprot
P05107 (ITGB2) S745 Uniprot
P05107 (ITGB2) T758 Uniprot
P05771-2 (PRKCB) S16 Uniprot
P05771-2 (PRKCB) T17 Uniprot
P05771-1 (PRKCB) T635 Uniprot
P05771 (PRKCB) T642 Uniprot
P05771-2 (PRKCB) S660 Uniprot
P05771 (PRKCB) S661 Uniprot
P06127 (CD5) T434 Uniprot
P06127 (CD5) T436 Uniprot
P06400 (RB1) S249 Uniprot
P06400 (RB1) T252 Uniprot
P06400 (RB1) S780 Uniprot
P06400 (RB1) S795 Uniprot
P06400 (RB1) T821 Uniprot
P06730-1 (EIF4E) S209 Uniprot
P07355-1 (ANXA2) S2 Uniprot
P07355-2 (ANXA2) S20 Uniprot
P07355 (ANXA2) S26 Uniprot
P10636-8 (MAPT) S258 Uniprot
P10636-8 (MAPT) S293 Uniprot
P10636-8 (MAPT) S324 Uniprot
P10636-8 (MAPT) S352 Uniprot
P11171-4 (EPB41) S312 Uniprot
P11388 (TOP2A) S29 Uniprot
P11473 (VDR) S51 Uniprot
P14598 (NCF1) S303 Uniprot
P14598 (NCF1) S304 Uniprot
P14598 (NCF1) S315 Uniprot
P14598 (NCF1) S320 Uniprot
P14598 (NCF1) S328 Uniprot
P14598 (NCF1) S359 Uniprot
P14598 (NCF1) S370 Uniprot
P14598 (NCF1) S379 Uniprot
P14679 (TYR) S523 Uniprot
P14679 (TYR) S527 Uniprot
P15336 (ATF2) S121 Uniprot
P16949 (STMN1) S25 Uniprot
P16949 (STMN1) S38 Uniprot
P17302 (GJA1) S368 Uniprot
P17677-1 (GAP43) S41 Uniprot
P19429 (TNNI3) S42 Uniprot
P19429 (TNNI3) S44 Uniprot
P19429 (TNNI3) T143 Uniprot
P19484 (TFEB) S462 Uniprot
P19484 (TFEB) S463 Uniprot
P19484 (TFEB) S467 Uniprot
P19484 (TFEB) S469 Uniprot
P20700 (LMNB1) S395 Uniprot
P20700 (LMNB1) S405 Uniprot
P21730 (C5AR1) S334 Uniprot
P26010-1 (ITGB7) T783 Uniprot
P28329-3 (CHAT) T255 Uniprot
P28329-3 (CHAT) S346 Uniprot
P28329-3 (CHAT) S347 Uniprot
P28329 (CHAT) T373 Uniprot
P28329-3 (CHAT) S440 Uniprot
P28329 (CHAT) S464 Uniprot
P28329 (CHAT) S465 Uniprot
P28329-3 (CHAT) S476 Uniprot
P28329 (CHAT) S558 Uniprot
P28329 (CHAT) S594 Uniprot
P28799 (GRN) S81 Uniprot
P29353 (SHC1) S36 Uniprot
P29474 (NOS3) T495 Uniprot
P29966 (MARCKS) S159 Uniprot
P29966 (MARCKS) S163 Uniprot
P29966 (MARCKS) S170 Uniprot
P30086 (PEBP1) S153 Uniprot
P31749 (AKT1) T308 Uniprot
P31749 (AKT1) S473 Uniprot
P34741 (SDC2) S187 Uniprot
P34741 (SDC2) S188 Uniprot
P35548 (MSX2) T135 Uniprot
P35548 (MSX2) T141 Uniprot
P35568 (IRS1) S323 Uniprot
P35568 (IRS1) S441 Uniprot
P35637 (FUS) S257 Uniprot
P41180 (CASR) T888 Uniprot
P41594-2 (GRM5) S840 Uniprot
P43629 (KIR3DL1) S415 Uniprot
P48736 (PIK3CG) S582 Uniprot
P49768 (PSEN1) S346 Uniprot
P49840 (GSK3A) S21 Uniprot
P49841 (GSK3B) S9 Uniprot
P55042 (RRAD) S214 Uniprot
P55042 (RRAD) S257 Uniprot
P55042 (RRAD) S290 Uniprot
P55042 (RRAD) S299 Uniprot
P56537-2 (EIF6) T215 Uniprot
P56537-1 (EIF6) T234 Uniprot
P56537 (EIF6) S235 Uniprot
P61764-1 (STXBP1) S306 Uniprot
P61764-2 (STXBP1) S313 Uniprot
P62491 (RAB11A) S177 Uniprot
P98082 (DAB2) S24 Uniprot
Q01844 (EWSR1) S266 Uniprot
Q03721 (KCNC4) S8 Uniprot
Q03721 (KCNC4) S9 Uniprot
Q03721 (KCNC4) S15 Uniprot
Q03721 (KCNC4) S21 Uniprot
Q05639 (EEF1A2) S53 Uniprot
Q06124-2 (PTPN11) S576 Uniprot
Q06124 (PTPN11) S580 Uniprot
Q06124 (PTPN11) S595 Uniprot
Q06187 (BTK) S180 Uniprot
Q07869 (PPARA) S179 Uniprot
Q07869 (PPARA) S230 Uniprot
Q12906 (ILF3) S647 Uniprot
Q13224 (GRIN2B) S1303 Uniprot
Q13224 (GRIN2B) S1323 Uniprot
Q14847 (LASP1) S146 Uniprot
Q16236 (NFE2L2) S40 Uniprot
Q16625-1 (OCLN) S340 Uniprot
Q16625 (OCLN) S490 Uniprot
Q5JVS0-1 (HABP4) T354 Uniprot
Q5JVS0 (HABP4) T375 Uniprot
Q5T7P8 (SYT6) T283 Uniprot
Q5T7P8 (SYT6) T417 Uniprot
Q5T7P8 (SYT6) T418 Uniprot
Q8NER1 (TRPV1) T705 Uniprot
Q92686 (NRGN) S36 Uniprot
Q96D31 (ORAI1) S27 Uniprot
Q96D31 (ORAI1) S30 Uniprot
Q96PH1-4 (NOX5) T494 Uniprot
Q96PH1-4 (NOX5) S498 Uniprot
Q99418-2 (CYTH2) S391 Uniprot
Q99418-1 (CYTH2) S392 Uniprot
Q9BXL7 (CARD11) S559 Uniprot
Q9BXL7 (CARD11) S644 Uniprot
Q9BXL7 (CARD11) S652 Uniprot
Q9H1D0 (TRPV6) S184 Uniprot
Q9H1D0 (TRPV6) T728 Uniprot
Q9P2D0 (IBTK) S1200 Uniprot
Q9P2D0 (IBTK) S1203 Uniprot
Q9UBS0 (RPS6KB2) S473 Uniprot
Q9UBU3 (GHRL) S41 Uniprot
Q9UQ80-1 (PA2G4) S363 Uniprot
Q9UQ80-1 (PA2G4) T366 Uniprot
Q9Y4H2 (IRS2) S306 Uniprot
Q9Y4H2 (IRS2) S679 Uniprot

研究背景

機能:

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.

PTMs:

Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade (By similarity).

細胞の位置付け:

Cytoplasm. Nucleus. Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with PDK1 (By similarity). Interacts in vitro with PRKCBP1. Interacts with PHLPP1 and PHLPP2; both proteins mediate its dephosphorylation. Interacts with KDM1A/LSD1, PKN1 and ANDR.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Morphine addiction.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > GABAergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

Restrictive clause

 

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