Phospho-Plastin L (Tyr300) Antibody - #AF7224
| 製品: | Phospho-Plastin L (Tyr300) Antibody |
| カタログ: | AF7224 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-Plastin L (Tyr300) |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 70kDa; 70kD(Calculated). |
| ユニプロット: | P13796 |
| RRID: | AB_2843664 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7224, RRID:AB_2843664.
折りたたみ／展開
CP64; L plastin; L-plastin; Larval cuticle protein 1; LC64P; LCP-1; LCP1; LPL; Lplastin; Lymphocyte cytosolic protein 1; Plastin 2; Plastin-2; PLS2; PLSL_HUMAN;
免疫原
Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T-lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.
- P13796 PLSL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARGSVSDEEMMELREAFAKVDTDGNGYISFNELNDLFKAACLPLPGYRVREITENLMATGDLDQDGRISFDEFIKIFHGLKSTDVAKTFRKAINKKEGICAIGGTSEQSSVGTQHSYSEEEKYAFVNWINKALENDPDCRHVIPMNPNTNDLFNAVGDGIVLCKMINLSVPDTIDERTINKKKLTPFTIQENLNLALNSASAIGCHVVNIGAEDLKEGKPYLVLGLLWQVIKIGLFADIELSRNEALIALLREGESLEDLMKLSPEELLLRWANYHLENAGCNKIGNFSTDIKDSKAYYHLLEQVAPKGDEEGVPAVVIDMSGLREKDDIQRAECMLQQAERLGCRQFVTATDVVRGNPKLNLAFIANLFNRYPALHKPENQDIDWGALEGETREERTFRNWMNSLGVNPRVNHLYSDLSDALVIFQLYEKIKVPVDWNRVNKPPYPKLGGNMKKLENCNYAVELGKNQAKFSLVGIGGQDLNEGNRTLTLALIWQLMRRYTLNILEEIGGGQKVNDDIIVNWVNETLREAKKSSSISSFKDPKISTSLPVLDLIDAIQPGSINYDLLKTENLNDDEKLNNAKYAISMARKIGARVYALPEDLVEVNPKMVMTVFACLMGKGMKRV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P13796 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | P17612 (PRKACA) , P51812 (RPS6KA3) , Q15418 (RPS6KA1) , Q05655 (PRKCD) | Uniprot |
| S7 | Phosphorylation | Uniprot | |
| K20 | Acetylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| Y28 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| K76 | Acetylation | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K82 | Acetylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K88 | Acetylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| T89 | Phosphorylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| Y118 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| Y276 | Phosphorylation | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| T291 | Phosphorylation | Uniprot | |
| K294 | Acetylation | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K297 | Acetylation | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| Y299 | Phosphorylation | Uniprot | |
| Y300 | Phosphorylation | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| K361 | Acetylation | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| Y374 | Phosphorylation | Uniprot | |
| K379 | Acetylation | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| T399 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| Y417 | Phosphorylation | Uniprot | |
| Y430 | Phosphorylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| K434 | Methylation | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| K444 | Methylation | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| K449 | Ubiquitination | Uniprot | |
| K455 | Acetylation | Uniprot | |
| K456 | Acetylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| K472 | Acetylation | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| K542 | Acetylation | Uniprot | |
| K542 | Ubiquitination | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| T548 | Phosphorylation | Uniprot | |
| S549 | Phosphorylation | Uniprot | |
| K579 | Acetylation | Uniprot | |
| K579 | Ubiquitination | Uniprot | |
| K584 | Acetylation | Uniprot | |
| K584 | Ubiquitination | Uniprot | |
| Y598 | Phosphorylation | Uniprot | |
| K610 | Ubiquitination | Uniprot | |
| T614 | Phosphorylation | Uniprot |
研究背景
Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
Cytoplasm>Cytoskeleton. Cell junction. Cell projection. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Relocalizes to the immunological synapse between peripheral blood T-lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28 (PubMed:17294403). Associated with the actin cytoskeleton at membrane ruffles. Relocalizes to actin-rich cell projections upon serine phosphorylation (PubMed:16636079).
Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T-lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.
Monomer. Interacts with AIF1 (By similarity). Interacts with actin.
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