OCT1 Antibody - #AF0224
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0224, RRID:AB_2833399.
折りたたみ／展開
FLJ42836; NF A1; NF-A1; Oct 1; Oct 1B; Oct-1; OCT1; Octamer binding protein 1; Octamer binding transcription factor 1; Octamer-binding protein 1; Octamer-binding transcription factor 1; OTF 1; OTF-1; OTF1; OTTHUMP00000032348; OTTHUMP00000032350; OTTHUMP00000032351; PO21; PO2F1; PO2F1_HUMAN; POU class 2 homeobox 1; POU domain class 2 transcription factor 1; POU domain, class 2, transcription factor 1; POU2F1;
免疫原
- P14859 PO2F1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQFVLVHPTTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQSQPSITLTSQPATPTRTIAATPIQTLPQSQSTPKRIDTPSLEEPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAENLSSDSSLSSPSALNSPGIEGLSRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEKEVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPTSLVATTPSLVTSSAATTLTVSPVLPLTSAAVTNLSVTGTSDTTSNNTATVISTAPPASSAVTSPSLSPSPSASASTSEASSASETSTTQTTSTPLSSPLGTSQVMVTASGLQTAAAAALQGAAQLPANASLAAMAAAAGLNPSLMAPSQFAAGGALLSLNPGTLSGALSPALMSNSTLATIQALASGGSLPITSLDATGNLVFANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASAGNSAPVASLHATSTSAESIQNSLFTVASASGAASTTTTASKAQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P14859 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S78 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S81 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S85 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S88 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S92 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T100 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S141 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S143 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S147 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T162 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S167 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T226 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S232 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T251 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T270 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K272 | Acetylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| S335 | Phosphorylation | Q9HC98 (NEK6) , Q9Y478 (PRKAB1) , Q13131 (PRKAA1) | Uniprot |
| S366 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Q13131 (PRKAA1) , P17612 (PRKACA) , P50613 (CDK7) | Uniprot |
| T445 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot |
研究背景
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
Phosphorylated by PRKDC.
Nucleus.
Ubiquitous. Isoform 2 is lymphocyte-specific.
Interacts with NR3C1, AR, PGR and HCFC1. Associates with the herpes simplex virus VP16-induced complex; binding to HCFC1 activates the viral transcriptional activator VP16 for association with POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes.
Belongs to the POU transcription factor family. Class-2 subfamily.
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