Phospho-TNNI3 (Ser23+Ser24) Antibody - #AF7307
| 製品: | Phospho-TNNI3 (Ser23+Ser24) Antibody |
| カタログ: | AF7307 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-TNNI3 (Ser23+Ser24) |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 26kDa; 24kD(Calculated). |
| ユニプロット: | P48787 |
| RRID: | AB_2843747 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7307, RRID:AB_2843747.
折りたたみ／展開
cardiac muscle; Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); Cardiomyopathy, familial hypertrophic, 7, included; CMD1FF; CMD2A; CMH7; cTnI; Familial hypertrophic cardiomyopathy 7; MGC116817; RCM1; Tn1; Tni; TNN I3; TNNC 1; TNNC1; TNNI3; TNNI3_HUMAN; Troponin I; Troponin I cardiac; Troponin I cardiac muscle; Troponin I cardiac muscle isoform; Troponin I type 3 cardiac; troponin I, cardiac 3; TroponinI; Ttroponin I type 3 (cardiac);
免疫原
A synthesized peptide derived from mouse TNNI3 around the phosphorylation site of Ser23+Ser24.
- P48787 TNNI3_MOUSE:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADESSDAAGEPQPAPAPVRRRSSANYRAYATEPHAKKKSKISASRKLQLKTLMLQIAKQEMEREAEERRGEKGRVLRTRCQPLELDGLGFEELQDLCRQLHARVDKVDEERYDVEAKVTKNITEIADLTQKIYDLRGKFKRPTLRRVRISADAMMQALLGTRAKESLDLRAHLKQVKKEDIEKENREVGDWRKNIDALSGMEGRKKKFEG
PTMs - P48787 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | P68404-2 (Prkcb) , P16054 (Prkce) , P05132 (Prkaca) , P70336 (Rock2) , P20444 (Prkca) , P18653 (Rps6ka1) | Uniprot |
| S24 | Phosphorylation | P68404-2 (Prkcb) , P18653 (Rps6ka1) , P05132 (Prkaca) , P20444 (Prkca) , P70336 (Rock2) , P16054 (Prkce) | Uniprot |
| Y27 | Phosphorylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| S43 | Phosphorylation | P68404-2 (Prkcb) , P20444 (Prkca) | Uniprot |
| S45 | Phosphorylation | P20444 (Prkca) , P68404-2 (Prkcb) | Uniprot |
| K51 | Ubiquitination | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| C81 | S-Nitrosylation | Uniprot | |
| C98 | S-Nitrosylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| Y134 | Phosphorylation | Uniprot | |
| T144 | Phosphorylation | P68404-2 (Prkcb) , P70336 (Rock2) , P20444 (Prkca) | Uniprot |
| S151 | Phosphorylation | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| K194 | Acetylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| S200 | Phosphorylation | Uniprot |
研究背景
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-32. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T) (By similarity). Phosphorylated at Ser-43 and Ser-45 by PRKCE; phosphorylation increases myocardium contractile dysfunction.
Interacts with TRIM63 (By similarity). Binds to actin and tropomyosin. Interacts with STK4/MST1 (By similarity).
Belongs to the troponin I family.
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