製品: Phospho-Cyclin E1 (Thr395) Antibody
カタログ: AF3235
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Cyclin E1 (Thr395)
アプリケーション: WB IHC IF/ICC
反応性: Human
予測: Bovine, Horse, Sheep
分子量: 48kDa; 47kD(Calculated).
ユニプロット: P24864
RRID: AB_2834661

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Bovine(82%), Horse(80%), Sheep(82%)
クローナリティ:
Polyclonal
特異性:
Phospho-Cyclin E1 (Thr395) Antibody detects endogenous levels of Cyclin E1 only when phosphorylated at Threonine 395.
RRID:
AB_2834661
引用形式: Affinity Biosciences Cat# AF3235, RRID:AB_2834661.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

CCNE; Ccne1; CCNE1_HUMAN; cyclin E variant ex5del; cyclin E variant ex7del; Cyclin E1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P24864 CCNE1_HUMAN:

Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

タンパク質の説明:
a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases.
タンパク質配列:
MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
82
Sheep
82
Horse
80
Pig
73
Xenopus
73
Chicken
73
Zebrafish
64
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24864 基板として

Site PTM Type Enzyme
S59 Phosphorylation
S73 Phosphorylation P49841 (GSK3B)
T77 Phosphorylation P49841 (GSK3B)
S90 Phosphorylation
S103 Phosphorylation
S381 Phosphorylation
S387 Phosphorylation P24941 (CDK2)
S391 Phosphorylation
T395 Phosphorylation P49841 (GSK3B) , P24941 (CDK2) , P49840 (GSK3A)
S399 Phosphorylation P24941 (CDK2)

PTMs - P24864 酵素として

Substrate Site Source
Q8TDN4-2 (CABLES1) S48 Uniprot

研究背景

機能:

Essential for the control of the cell cycle at the G1/S (start) transition.

PTMs:

Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.

Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

サブユニット構造:

Interacts with CDK2 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation. Interacts with INCA1.

タンパク質ファミリー:

Belongs to the cyclin family. Cyclin E subfamily.

研究領域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

参考文献

1). AKAP95 regulates ubiquitination and degradation of cyclin Ds/Es, influencing the G1/S transition of lung cancer cells. Molecular carcinogenesis, 2024 (PubMed: 38923703) [IF=4.6]

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