Phospho-ACC1 (Thr1196) Antibody - #AF7364
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7364, RRID:AB_2843804.
折りたたみ／展開
ACAC;ACACA;ACACA_HUMAN;ACC alpha;ACC 1;ACC-alpha;ACC1;ACCA;Acetyl CoA carboxylase 1;Acetyl CoA carboxylase alpha;Acetyl Coenzyme A carboxylase alpha;Biotin carboxylase;COA1;HACC275 antibody;
免疫原
Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
- Q13085 ACACA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDEPSPLAQPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHIRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKRILNVPQELYEKGYVKDVDDGLQAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDSPIDFEDSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGCVLAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFSDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVDHGIRRLTFLVAQKDFRKQVNYEVDRRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTFKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVSANSGARIGLAEEIRHMFHVAWVDPEDPYKGYRYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGIGPENLRGSGMIAGESSLAYNEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHCTVCDDFEGVFTVLHWLSYMPKSVHSSVPLLNSKDPIDRIIEFVPTKTPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECCQPVLVYIPPQAELRGGSWVVIDSSINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIHLAERLGTPELSTAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVISDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLAEWLEKQLTEEDGVHSVIEENIKCISRDYVLKQIRSLVQANPEVAMDSIIHMTQHISPTQRAEVIRILSTMDSPST
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13085 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| T58 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S78 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| S80 | Phosphorylation | P54646 (PRKAA2) , Q13131 (PRKAA1) | Uniprot |
| K86 | Ubiquitination | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| Y290 | Phosphorylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| K410 | Ubiquitination | Uniprot | |
| Y417 | Phosphorylation | Uniprot | |
| Y434 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| K520 | Ubiquitination | Uniprot | |
| K579 | Ubiquitination | Uniprot | |
| T610 | Phosphorylation | Uniprot | |
| Y676 | Phosphorylation | Uniprot | |
| Y734 | Phosphorylation | Uniprot | |
| K747 | Ubiquitination | Uniprot | |
| S756 | Phosphorylation | Uniprot | |
| K817 | Ubiquitination | Uniprot | |
| K825 | Ubiquitination | Uniprot | |
| S835 | Phosphorylation | Uniprot | |
| S841 | Phosphorylation | Uniprot | |
| Y855 | Phosphorylation | Uniprot | |
| Y867 | Phosphorylation | Uniprot | |
| K879 | Ubiquitination | Uniprot | |
| S893 | Phosphorylation | Uniprot | |
| S907 | Phosphorylation | Uniprot | |
| K916 | Ubiquitination | Uniprot | |
| S977 | Phosphorylation | Uniprot | |
| Y994 | Phosphorylation | Uniprot | |
| T1042 | Phosphorylation | Uniprot | |
| T1073 | Phosphorylation | Uniprot | |
| K1076 | Ubiquitination | Uniprot | |
| T1196 | Phosphorylation | Uniprot | |
| S1201 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| Y1209 | Phosphorylation | Uniprot | |
| S1216 | Phosphorylation | Uniprot | |
| S1218 | Phosphorylation | Uniprot | |
| T1227 | Phosphorylation | Uniprot | |
| S1257 | Phosphorylation | Uniprot | |
| S1259 | Phosphorylation | Uniprot | |
| S1263 | Phosphorylation | Uniprot | |
| T1265 | Phosphorylation | Uniprot | |
| S1273 | Phosphorylation | Uniprot | |
| K1316 | Ubiquitination | Uniprot | |
| K1334 | Acetylation | Uniprot | |
| K1334 | Ubiquitination | Uniprot | |
| K1338 | Ubiquitination | Uniprot | |
| Y1342 | Phosphorylation | Uniprot | |
| K1354 | Ubiquitination | Uniprot | |
| Y1370 | Phosphorylation | Uniprot | |
| K1435 | Ubiquitination | Uniprot | |
| S1438 | Phosphorylation | Uniprot | |
| Y1441 | Phosphorylation | Uniprot | |
| Y1499 | Phosphorylation | Uniprot | |
| K1524 | Ubiquitination | Uniprot | |
| K1580 | Acetylation | Uniprot | |
| K1586 | Ubiquitination | Uniprot | |
| S1720 | Phosphorylation | Uniprot | |
| Y1745 | Phosphorylation | Uniprot | |
| K1759 | Acetylation | Uniprot | |
| K1759 | Ubiquitination | Uniprot | |
| K1781 | Ubiquitination | Uniprot | |
| K1788 | Acetylation | Uniprot | |
| K1788 | Ubiquitination | Uniprot | |
| S1844 | Phosphorylation | Uniprot | |
| K1856 | Acetylation | Uniprot | |
| K1856 | Ubiquitination | Uniprot | |
| S1908 | Phosphorylation | Uniprot | |
| K1916 | Ubiquitination | Uniprot | |
| K1929 | Ubiquitination | Uniprot | |
| K2031 | Acetylation | Uniprot | |
| K2031 | Ubiquitination | Uniprot | |
| R2047 | Methylation | Uniprot | |
| S2099 | Phosphorylation | Uniprot | |
| Y2108 | Phosphorylation | Uniprot | |
| K2127 | Acetylation | Uniprot | |
| K2127 | Ubiquitination | Uniprot | |
| K2135 | Ubiquitination | Uniprot | |
| Y2144 | Phosphorylation | Uniprot | |
| T2153 | Phosphorylation | Uniprot | |
| K2209 | Ubiquitination | Uniprot | |
| K2231 | Ubiquitination | Uniprot | |
| K2268 | Ubiquitination | Uniprot | |
| K2293 | Ubiquitination | Uniprot | |
| K2302 | Ubiquitination | Uniprot | |
| S2318 | Phosphorylation | Uniprot | |
| S2343 | Phosphorylation | Uniprot | |
| S2345 | Phosphorylation | Uniprot |
研究背景
Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.
Phosphorylation on Ser-1263 is required for interaction with BRCA1.
Phosphorylation at Ser-80 by AMPK inactivates enzyme activity.
The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.
Cytoplasm>Cytosol.
Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity.
Consists of an N-terminal biotin carboxylation/carboxylase (BC) domain that catalyzes the ATP-dependent transient carboxylation of the biotin covalently attached to the central biotinyl-binding/biotin carboxyl carrier (BCC) domain (Probable). The C-terminal carboxyl transferase (CT) domain catalyzes the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA to produce malonyl-CoA (Probable).
研究領域
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Metabolism > Lipid metabolism > Fatty acid biosynthesis.
· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.
· Metabolism > Carbohydrate metabolism > Propanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Fatty acid metabolism.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
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