製品: Phospho-TDP43 (Ser409) Antibody
カタログ: AF7365
タンパク質の説明: Rabbit polyclonal antibody to Phospho-TDP43 (Ser409)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Zebrafish, Dog, Chicken
分子量: 43kDa; 45kD(Calculated).
ユニプロット: Q13148
RRID: AB_2843805

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(90%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-TDP43 (Ser409) Antibody detects endogenous levels of TDP43 only when phosphorylated at Ser409.
RRID:
AB_2843805
引用形式: Affinity Biosciences Cat# AF7365, RRID:AB_2843805.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ALS10; OTTHUMP00000002171; OTTHUMP00000002172; OTTHUMP00000002173; TADBP_HUMAN; TAR DNA binding protein 43; TAR DNA binding protein; TAR DNA-binding protein 43; TARDBP; TDP 43; TDP-43; TDP43;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q13148 TADBP_HUMAN:

Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.

タンパク質配列:
MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNGGFGSSMDSKSSGWGM

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Chicken
100
Zebrafish
90
Pig
0
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13148 基板として

Site PTM Type Enzyme
S2 Phosphorylation P48729 (CSNK1A1)
Y4 Phosphorylation P48729 (CSNK1A1)
T25 Phosphorylation P48729 (CSNK1A1)
S48 Phosphorylation
K84 Sumoylation
K84 Ubiquitination
T88 Phosphorylation P48729 (CSNK1A1)
S91 Phosphorylation P48729 (CSNK1A1)
S92 Phosphorylation P48729 (CSNK1A1)
K95 Acetylation
K95 Sumoylation
K95 Ubiquitination
K102 Sumoylation
K102 Ubiquitination
K114 Ubiquitination
T116 Phosphorylation P48729 (CSNK1A1)
K121 Sumoylation
K121 Ubiquitination
K136 Acetylation
K140 Acetylation
K140 Ubiquitination
K145 Acetylation
K145 Sumoylation
K145 Ubiquitination
T153 Phosphorylation
Y155 Phosphorylation
K160 Sumoylation
K160 Ubiquitination
K176 Sumoylation
K176 Ubiquitination
K181 Methylation
K181 Sumoylation
K181 Ubiquitination
S183 Phosphorylation P48729 (CSNK1A1)
K192 Acetylation
K192 Sumoylation
K192 Ubiquitination
K224 Ubiquitination
S242 Phosphorylation P48729 (CSNK1A1)
S254 Phosphorylation P48729 (CSNK1A1)
K263 Sumoylation
K263 Ubiquitination
S273 Phosphorylation P48729 (CSNK1A1)
R275 Methylation
S292 Phosphorylation P48729 (CSNK1A1)
R293 Methylation
S305 Phosphorylation P48729 (CSNK1A1)
S317 Phosphorylation
S333 Phosphorylation
S342 Phosphorylation P48729 (CSNK1A1)
S347 Phosphorylation P48729 (CSNK1A1)
S350 Phosphorylation P48729 (CSNK1A1)
S369 Phosphorylation P48729 (CSNK1A1)
S375 Phosphorylation P48729 (CSNK1A1)
S377 Phosphorylation P48729 (CSNK1A1)
S379 Phosphorylation P48729 (CSNK1A1) , P68400 (CSNK2A1)
S387 Phosphorylation P48729 (CSNK1A1)
S389 Phosphorylation P48729 (CSNK1A1)
S393 Phosphorylation P48729 (CSNK1A1)
S395 Phosphorylation P48729 (CSNK1A1)
S403 Phosphorylation P48729 (CSNK1A1)
S404 Phosphorylation P48729 (CSNK1A1)
S407 Phosphorylation P48729 (CSNK1A1)
K408 Sumoylation
S409 Phosphorylation P68400 (CSNK2A1) , P48729 (CSNK1A1)
S410 Phosphorylation P68400 (CSNK2A1) , P48729 (CSNK1A1)

研究背景

機能:

RNA-binding protein that is involved in various steps of RNA biogenesis and processing. Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs. In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases. Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts. Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening. In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival. Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins. Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner.

PTMs:

Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. Phosphorylated upon cellular stress.

Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.

Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.

細胞の位置付け:

Nucleus. Cytoplasm. Cytoplasm>Stress granule.
Note: Continuously travels in and out of the nucleus (PubMed:18957508). Localizes to stress granules in response to oxidative stress (PubMed:19765185).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.

サブユニット構造:

Homodimer. Homooligomer (via its N-terminal domain). Interacts with BRDT (By similarity). Binds specifically to pyrimidine-rich motifs of TAR DNA and to single stranded TG repeated sequences. Binds to RNA, specifically to UG repeated sequences with a minimun of six contiguous repeats. Interacts with ATNX2; the interaction is RNA-dependent. Interacts with MATR3. Interacts with UBQLN2. Interacts with HNRNPA2B1. Interacts with ZNF106 (By similarity). Interacts with CNOT7/CAF1. Interacts with CRY2.

タンパク質ファミリー:

Consists of an N-terminal domain (NTD) and two tandem RNA recognition motifs, RRM1 and RRM2, followed by a C-terminal glycine-rich region.

Contains a nuclear localization sequence and is mostly nuclear; however, its nuclear export sequence permits it to transport mRNAs to the cytoplasm and even to synapses as part of neuronal granules.

参考文献

1). Genome-wide identification of the genetic basis of amyotrophic lateral sclerosis. , 2022

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