Phospho-CaMK1 alpha (Thr177) Antibody - #AF7381
| 製品: | Phospho-CaMK1 alpha (Thr177) Antibody |
| カタログ: | AF7381 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-CaMK1 alpha (Thr177) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 41kDa; 41kD(Calculated). |
| ユニプロット: | Q14012 |
| RRID: | AB_2843821 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7381, RRID:AB_2843821.
折りたたみ／展開
Calcium / calmodulin dependent protein kinase 1; Calcium / calmodulin dependent protein kinase I; Calcium / calmodulin dependent protein kinase type 1; Calcium/calmodulin dependent protein kinase 1; Calcium/calmodulin dependent protein kinase I; Calcium/calmodulin dependent protein kinase type 1; Calcium/calmodulin-dependent protein kinase type 1; CaM K1; CaM KI; CaM kinase 1 alpha; CAM kinase 1; CaM kinase I alpha; CaM kinase I; CaM-KI; CaMK 1; CAMK I; CaMK1 alpha; CAMK1; CAMK1 PEN; CaMKI alpha; CaMKI-alpha; KCC1A_HUMAN; MGC120317; MGC120318;
免疫原
Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
- Q14012 KCC1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLGAVEGPRWKQAEDIRDIYDFRDVLGTGAFSEVILAEDKRTQKLVAIKCIAKEALEGKEGSMENEIAVLHKIKHPNIVALDDIYESGGHLYLIMQLVSGGELFDRIVEKGFYTERDASRLIFQVLDAVKYLHDLGIVHRDLKPENLLYYSLDEDSKIMISDFGLSKMEDPGSVLSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPFYDENDAKLFEQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDPEKRFTCEQALQHPWIAGDTALDKNIHQSVSEQIKKNFAKSKWKQAFNATAVVRHMRKLQLGTSQEGQGQTASHGELLTPVAGGPAAGCCCRDCCVEPGTELSPTLPHQL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14012 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y20 | Phosphorylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| T177 | Phosphorylation | Q8N5S9 (CAMKK1) , Q96RR4 (CAMKK2) , Q14012 (CAMK1) | Uniprot |
| Y235 | Phosphorylation | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot |
PTMs - Q14012 酵素として
| Substrate | Site | Source |
|---|---|---|
| O43432 (EIF4G3) | S1156 | Uniprot |
| P05783 (KRT18) | S53 | Uniprot |
| P16220 (CREB1) | S133 | Uniprot |
| P17600 (SYN1) | S9 | Uniprot |
| P18846 (ATF1) | S63 | Uniprot |
| P24844 (MYL9) | S20 | Uniprot |
| P29475 (NOS1) | S746 | Uniprot |
| P29475-1 (NOS1) | S852 | Uniprot |
| P39019 (RPS19) | S59 | Uniprot |
| P46527 (CDKN1B) | T157 | Uniprot |
| P46527 (CDKN1B) | T198 | Uniprot |
| P49757 (NUMB) | S276 | Uniprot |
| P49757 (NUMB) | S295 | Uniprot |
| P57059 (SIK1) | T322 | Uniprot |
| Q05469 (LIPE) | S855 | Uniprot |
| Q14012 (CAMK1) | T177 | Uniprot |
| Q14155 (ARHGEF7) | S694 | Uniprot |
| Q7KZI7 (MARK2) | S91 | Uniprot |
| Q7KZI7 (MARK2) | S92 | Uniprot |
| Q7KZI7 (MARK2) | S93 | Uniprot |
| Q7KZI7 (MARK2) | T294 | Uniprot |
| Q9NP62 (GCM1) | S47 | Uniprot |
| Q9UKV0 (HDAC9) | S220 | Uniprot |
| Q9UKV0 (HDAC9) | S451 | Uniprot |
| Q9UQL6 (HDAC5) | S259 | Uniprot |
| Q9UQL6-3 (HDAC5) | S260 | Uniprot |
| Q9UQL6 (HDAC5) | S498 | Uniprot |
| Q9UQL6-3 (HDAC5) | S499 | Uniprot |
| Q9UQM7-2 (CAMK2A) | S332 | Uniprot |
研究背景
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
Phosphorylated by CaMKK1 and CaMKK2 on Thr-177.
Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.
Cytoplasm. Nucleus.
Note: Predominantly cytoplasmic.
Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
Monomer. Interacts with XPO1 (By similarity). Interacts with MARK2, ARHGEF7/BETAPIX and GIT1.
The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
研究領域
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
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