Phospho-PYK2 (Tyr402) Antibody - #AF7384
製品: | Phospho-PYK2 (Tyr402) Antibody |
カタログ: | AF7384 |
タンパク質の説明: | Rabbit polyclonal antibody to Phospho-PYK2 (Tyr402) |
アプリケーション: | WB IHC |
反応性: | Human, Mouse, Rat |
予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
分子量: | 135kDa; 116kD(Calculated). |
ユニプロット: | Q14289 |
RRID: | AB_2843824 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7384, RRID:AB_2843824.
折りたたみ/展開
CADTK; CAK-beta; CAKB; CAKbeta; Calcium regulated non receptor proline rich tyrosine kinase; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; E430023O05Rik; EC 2.7.10.2; FADK 2; FADK2; FAK2; FAK2_HUMAN; Focal adhesion kinase 2; MGC124628; PKB; Proline-rich tyrosine kinase 2; Protein kinase B; Protein Tyrosine Kinase 2 Beta; Protein-tyrosine kinase 2-beta; PTK; PTK2B; PTK2B protein tyrosine kinase 2 beta; PYK2; RAFTK; RAFTK2; Related adhesion focal tyrosine kinase;
免疫原
Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.
- Q14289 FAK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGVSEPLSRVKLGTLRRPEGPAEPMVVVPVDVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQTEIREIITSILLSGRIGPNIRLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDFMESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGLDLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKFFNTLAGFANIDQETYRCELIQGWNITVDLVIGPKGIRQLTSQDAKPTCLAEFKQIRSIRCLPLEEGQAVLQLGIEGAPQALSIKTSSLAEAENMADLIDGYCRLQGEHQGSLIIHPRKDGEKRNSLPQIPMLNLEARRSHLSESCSIESDIYAEIPDETLRRPGGPQYGIAREDVVLNRILGEGFFGEVYEGVYTNHKGEKINVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEPTWIIMELYPYGELGHYLERNKNSLKVLTLVLYSLQICKAMAYLESINCVHRDIAVRNILVASPECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLENKDVIGVLEKGDRLPKPDLCPPVLYTLMTRCWDYDPSDRPRFTELVCSLSDVYQMEKDIAMEQERNARYRTPKILEPTAFQEPPPKPSRPKYRPPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIQPSSREEAQQLWEAEKVKMRQILDKQQKQMVEDYQWLRQEEKSLDPMVYMNDKSPLTPEKEVGYLEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYLNVMELVRAVLELKNELCQLPPEGYVVVVKNVGLTLRKLIGSVDDLLPSLPSSSRTEIEGTQKLLNKDLAELINKMRLAQQNAVTSLSEECKRQMLTASHTLAVDAKNLLDAVDQAKVLANLAHPPAE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14289 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
Phosphorylation | Uniprot | ||
S2 | Phosphorylation | Uniprot | |
S9 | Phosphorylation | Uniprot | |
K12 | Ubiquitination | Uniprot | |
T15 | Phosphorylation | Uniprot | |
K54 | Ubiquitination | Uniprot | |
K188 | Ubiquitination | Uniprot | |
K198 | Ubiquitination | Uniprot | |
K206 | Ubiquitination | Uniprot | |
K216 | Ubiquitination | Uniprot | |
K295 | Ubiquitination | Uniprot | |
K303 | Ubiquitination | Uniprot | |
S332 | Phosphorylation | Uniprot | |
S361 | Phosphorylation | Uniprot | |
S375 | Phosphorylation | Uniprot | |
S394 | Phosphorylation | Uniprot | |
S396 | Phosphorylation | Uniprot | |
S399 | Phosphorylation | Uniprot | |
Y402 | Phosphorylation | Q14289 (PTK2B) , P43405 (SYK) , P12931 (SRC) | Uniprot |
T409 | Phosphorylation | Uniprot | |
Y418 | Phosphorylation | Uniprot | |
Y440 | Phosphorylation | Uniprot | |
K451 | Ubiquitination | Uniprot | |
K457 | Ubiquitination | Uniprot | |
S473 | Phosphorylation | Uniprot | |
K479 | Ubiquitination | Uniprot | |
S530 | Phosphorylation | Uniprot | |
S559 | Phosphorylation | Uniprot | |
K564 | Acetylation | Uniprot | |
K564 | Ubiquitination | Uniprot | |
S571 | Phosphorylation | Uniprot | |
Y573 | Phosphorylation | Uniprot | |
Y579 | Phosphorylation | Uniprot | |
Y580 | Phosphorylation | Uniprot | |
K581 | Ubiquitination | Uniprot | |
S583 | Phosphorylation | Uniprot | |
K640 | Ubiquitination | Uniprot | |
Y683 | Phosphorylation | Uniprot | |
Y699 | Phosphorylation | Uniprot | |
T701 | Phosphorylation | Uniprot | |
K703 | Ubiquitination | Uniprot | |
S718 | Phosphorylation | Uniprot | |
Y722 | Phosphorylation | Uniprot | |
S746 | Phosphorylation | Uniprot | |
S747 | Phosphorylation | Uniprot | |
T749 | Phosphorylation | Uniprot | |
S752 | Phosphorylation | Uniprot | |
Y756 | Phosphorylation | Uniprot | |
S758 | Phosphorylation | Uniprot | |
S762 | Phosphorylation | Uniprot | |
T765 | Phosphorylation | Uniprot | |
S778 | Phosphorylation | Uniprot | |
K801 | Ubiquitination | Uniprot | |
K810 | Ubiquitination | Uniprot | |
Y819 | Phosphorylation | Uniprot | |
S828 | Phosphorylation | Uniprot | |
Y834 | Phosphorylation | Uniprot | |
S839 | Phosphorylation | Uniprot | |
T842 | Phosphorylation | Uniprot | |
Y849 | Phosphorylation | Uniprot | |
T853 | Phosphorylation | Uniprot | |
K858 | Ubiquitination | Uniprot | |
S866 | Phosphorylation | Uniprot | |
Y881 | Phosphorylation | P12931 (SRC) | Uniprot |
Y906 | Phosphorylation | Uniprot | |
K911 | Ubiquitination | Uniprot | |
S923 | Phosphorylation | Uniprot | |
K944 | Ubiquitination | Uniprot | |
K948 | Ubiquitination | Uniprot | |
K956 | Ubiquitination | Uniprot | |
K973 | Ubiquitination | Uniprot | |
K998 | Ubiquitination | Uniprot |
PTMs - Q14289 酵素として
Substrate | Site | Source |
---|---|---|
O15259 (NPHP1) | Y46 | Uniprot |
O15259 (NPHP1) | Y349 | Uniprot |
O15259 (NPHP1) | Y721 | Uniprot |
O43294 (TGFB1I1) | Y60 | Uniprot |
P29474 (NOS3) | Y657 | Uniprot |
P37840 (SNCA) | Y125 | Uniprot |
Q05397 (PTK2) | Y407 | Uniprot |
Q14289-1 (PTK2B) | S375 | Uniprot |
Q14289-1 (PTK2B) | Y402 | Uniprot |
Q14289-2 (PTK2B) | Y579 | Uniprot |
Q14289-1 (PTK2B) | Y580 | Uniprot |
Q14289-1 (PTK2B) | S778 | Uniprot |
Q14289-2 (PTK2B) | Y839 | Uniprot |
Q14289-1 (PTK2B) | Y881 | Uniprot |
Q658W2 (DKFZp666O0110) | Y429 | Uniprot |
Q9ULH1 (ASAP1) | Y767 | Uniprot |
Q9ULZ3 (PYCARD) | Y146 | Uniprot |
研究背景
Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.
Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration; this activation is indirect and may be mediated by production of reactive oxygen species (ROS). Tyr-402 is the major autophosphorylation site, but other kinases can also phosphorylate Tyr-402. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-402 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-579; Tyr-580 and Tyr-881. Phosphorylation at Tyr-881 is important for interaction with GRB2. Phosphorylated on tyrosine residues upon activation of FGR and PKC. Recruitment by NPHP1 to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity. Phosphorylation by MYLK promotes ITGB2 activation and is thus essential to trigger neutrophil transmigration during lung injury. Dephosphorylated by PTPN12.
Cytoplasm. Cytoplasm>Perinuclear region. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Focal adhesion. Cell projection>Lamellipodium. Cytoplasm>Cell cortex. Nucleus.
Note: Interaction with NPHP1 induces the membrane-association of the kinase. Colocalizes with integrins at the cell periphery.
Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.
Homodimer, or homooligomer. Interacts with SIRPA and SH2D3C. Interacts with ARHGAP10. Interacts with DLG4 (By similarity). Interacts with KCNA2 (By similarity). Interacts with NPHP1, ASAP1, ASAP2, ARHGAP26, SKAP2 and TGFB1I1. The Tyr-402 phosphorylated form interacts with SRC (via SH2 domain) and SRC family members. Forms a signaling complex with EPHA1, LCK and phosphatidylinositol 3-kinase; upon activation by EFNA1. Interacts with GRB2 (via SH2 domain). Interacts with P53/TP53 and MDM2. Interacts with MYLK. Interacts with BCAR1. Interacts with PDPK1. Interacts (hypophosphorylated) with PXN. Interacts with RB1CC1. Interacts with RHOU. Interacts with VAV1. Interacts with LPXN and PTPN12.
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
研究領域
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
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