Phospho-NCoA2 (Ser698/Ser699) Antibody - #AF7394
| 製品: | Phospho-NCoA2 (Ser698/Ser699) Antibody |
| カタログ: | AF7394 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-NCoA2 (Ser698/Ser699) |
| アプリケーション: | WB IHC IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 175kDa; 159kD(Calculated). |
| ユニプロット: | Q15596 |
| RRID: | AB_2843834 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7394, RRID:AB_2843834.
折りたたみ／展開
bHLHe75; Class E basic helix-loop-helix protein 75; glucocorticoid receptor interacting protein 1; GRIP 1; GRIP1; hTIF2; MED1; MGC138808; NCoA 2; NCoA-2; Ncoa2; NCOA2_HUMAN; Nuclear receptor coactivator 2; Oncogene FGR; p160; SRC 2; src2; Steroid receptor coactivator 2; TIF 2; TIF2; Transcriptional intermediary factor 2;
免疫原
- Q15596 NCOA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGMGENTSDPSRAETRKRKECPDQLGPSPKRNTEKRNREQENKYIEELAELIFANFNDIDNFNFKPDKCAILKETVKQIRQIKEQEKAAAANIDEVQKSDVSSTGQGVIDKDALGPMMLEALDGFFFVVNLEGNVVFVSENVTQYLRYNQEELMNKSVYSILHVGDHTEFVKNLLPKSIVNGGSWSGEPPRRNSHTFNCRMLVKPLPDSEEEGHDNQEAHQKYETMQCFAVSQPKSIKEEGEDLQSCLICVARRVPMKERPVLPSSESFTTRQDLQGKITSLDTSTMRAAMKPGWEDLVRRCIQKFHAQHEGESVSYAKRHHHEVLRQGLAFSQIYRFSLSDGTLVAAQTKSKLIRSQTTNEPQLVISLHMLHREQNVCVMNPDLTGQTMGKPLNPISSNSPAHQALCSGNPGQDMTLSSNINFPINGPKEQMGMPMGRFGGSGGMNHVSGMQATTPQGSNYALKMNSPSQSSPGMNPGQPTSMLSPRHRMSPGVAGSPRIPPSQFSPAGSLHSPVGVCSSTGNSHSYTNSSLNALQALSEGHGVSLGSSLASPDLKMGNLQNSPVNMNPPPLSKMGSLDSKDCFGLYGEPSEGTTGQAESSCHPGEQKETNDPNLPPAVSSERADGQSRLHDSKGQTKLLQLLTTKSDQMEPSPLASSLSDTNKDSTGSLPGSGSTHGTSLKEKHKILHRLLQDSSSPVDLAKLTAEATGKDLSQESSSTAPGSEVTIKQEPVSPKKKENALLRYLLDKDDTKDIGLPEITPKLERLDSKTDPASNTKLIAMKTEKEEMSFEPGDQPGSELDNLEEILDDLQNSQLPQLFPDTRPGAPAGSVDKQAIINDLMQLTAENSPVTPVGAQKTALRISQSTFNNPRPGQLGRLLPNQNLPLDITLQSPTGAGPFPPIRNSSPYSVIPQPGMMGNQGMIGNQGNLGNSSTGMIGNSASRPTMPSGEWAPQSSAVRVTCAATTSAMNRPVQGGMIRNPAASIPMRPSSQPGQRQTLQSQVMNIGPSELEMNMGGPQYSQQQAPPNQTAPWPESILPIDQASFASQNRQPFGSSPDDLLCPHPAAESPSDEGALLDQLYLALRNFDGLEEIDRALGIPELVSQSQAVDPEQFSSQDSNIMLEQKAPVFPQQYASQAQMAQGSYSPMQDPNFHTMGQRPSYATLRMQPRPGLRPTGLVQNQPNQLRLQLQHRLQAQQNRQPLMNQISNVSNVNLTLRPGVPTQAPINAQMLAQRQREILNQHLRQRQMHQQQQVQQRTLMMRGQGLNMTPSMVAPSGMPATMSNPRIPQANAQQFPFPPNYGISQQPDPGFTGATTPQSPLMSPRMAHTQSPMMQQSQANPAYQAPSDINGWAQGNMGGNSMFSQQSPPHFGQQANTSMYSNNMNINVSMATNTGGMSSMNQMTGQISMTSVTSVPTSGLSSMGPEQVNDPALRGGNLFPNQLPGMDMIKQEGDTTRKYC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15596 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K31 | Acetylation | Uniprot | |
| T76 | Phosphorylation | Uniprot | |
| K78 | Acetylation | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| K239 | Sumoylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| T456 | Phosphorylation | Uniprot | |
| T457 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | P68400 (CSNK2A1) , P50750 (CDK9) | Uniprot |
| S471 | Phosphorylation | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| S487 | Phosphorylation | P50750 (CDK9) , P68400 (CSNK2A1) | Uniprot |
| R489 | Methylation | Uniprot | |
| S493 | Phosphorylation | P50750 (CDK9) , P68400 (CSNK2A1) | Uniprot |
| S499 | Phosphorylation | P50750 (CDK9) , P68400 (CSNK2A1) | Uniprot |
| R501 | Methylation | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| S565 | Phosphorylation | Uniprot | |
| S579 | Phosphorylation | Uniprot | |
| K636 | Acetylation | Uniprot | |
| K640 | Acetylation | Uniprot | |
| K640 | Sumoylation | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| S655 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| S668 | Phosphorylation | Uniprot | |
| T669 | Phosphorylation | Uniprot | |
| S671 | Phosphorylation | Uniprot | |
| S675 | Phosphorylation | Uniprot | |
| T681 | Phosphorylation | Uniprot | |
| S682 | Phosphorylation | Uniprot | |
| S697 | Phosphorylation | Uniprot | |
| S698 | Phosphorylation | Uniprot | |
| S699 | Phosphorylation | Uniprot | |
| K705 | Methylation | Uniprot | |
| K705 | Ubiquitination | Uniprot | |
| K713 | Acetylation | Uniprot | |
| S716 | Phosphorylation | Uniprot | |
| S719 | Phosphorylation | Uniprot | |
| S721 | Phosphorylation | Uniprot | |
| T722 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| T729 | Phosphorylation | Uniprot | |
| K731 | Sumoylation | Uniprot | |
| S736 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) , Q16539 (MAPK14) | Uniprot |
| K738 | Acetylation | Uniprot | |
| K739 | Acetylation | Uniprot | |
| Y747 | Phosphorylation | Uniprot | |
| K765 | Sumoylation | Uniprot | |
| S771 | Phosphorylation | Uniprot | |
| T773 | Phosphorylation | Uniprot | |
| K780 | Acetylation | Uniprot | |
| K785 | Acetylation | Uniprot | |
| K785 | Sumoylation | Uniprot | |
| K785 | Ubiquitination | Uniprot | |
| K788 | Sumoylation | Uniprot | |
| T847 | Phosphorylation | Uniprot | |
| S851 | Phosphorylation | Uniprot | |
| T854 | Phosphorylation | Uniprot | |
| R874 | Methylation | Uniprot | |
| K1129 | Sumoylation | Uniprot | |
| R1162 | Methylation | Uniprot | |
| R1169 | Methylation | Uniprot | |
| R1173 | Methylation | Uniprot | |
| R1177 | Methylation | Uniprot | |
| R1190 | Methylation | Uniprot | |
| R1196 | Methylation | Uniprot | |
| R1203 | Methylation | Uniprot | |
| R1221 | Methylation | Uniprot | |
| R1240 | Methylation | Uniprot | |
| R1248 | Methylation | Uniprot | |
| R1250 | Methylation | Uniprot | |
| R1261 | Methylation | Uniprot | |
| R1266 | Methylation | Uniprot | |
| K1454 | Sumoylation | Uniprot |
研究背景
Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).
Nucleus.
Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts (via C-terminus) with CREBBP. Interacts with ESR1, HIF1A, NCOA1, APEX, NR3C1, NR3C2, CARM1, RARA, and RXRA. Present in a complex containing CARM1 and EP300/P300. Interacts with CASP8AP2 and TTLL5/STAMP. Interacts with PSMB9 and DDX5. Interacts (via LXXLL 1, 2 and 3 motifs) with RORA and RORC (via AF-2 motif). Interacts with RWDD3. Interacts with CLOCK and ARNTL/BMAL1 (By similarity). Interacts with NR4A3; potentiates the activity of the NR4A3 (By similarity). Interacts with NR1H3.
Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.
The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.
Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.
Belongs to the SRC/p160 nuclear receptor coactivator family.
研究領域
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
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