Phospho-GRK1 (Ser21) Antibody - #AF7399

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製品: Phospho-GRK1 (Ser21) Antibody
カタログ: AF7399
タンパク質の説明: Rabbit polyclonal antibody to Phospho-GRK1 (Ser21)
アプリケーション: WB
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
分子量: 60kDa; 64kD(Calculated).
ユニプロット: Q15835
RRID: AB_2843839

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(100%), Bovine(88%), Horse(88%), Rabbit(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-GRK1 (Ser21) Antibody detects endogenous levels of GRK1 only when phosphorylated at Ser21.
RRID:
AB_2843839
引用形式: Affinity Biosciences Cat# AF7399, RRID:AB_2843839.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

EC=2.7.11.14; G protein-coupled receptor kinase 1; GPRK1; Grk1; Rhodopsin kinase; RHOK; RK; RK_HUMAN;

免疫原

免疫原:
Uniprot:

Q15835(GRK1_HUMAN) >>Visit HPA database.

遺伝子(ID):
GRK1(6011)
発現特異性:
Q15835 GRK1_HUMAN:

Retinal-specific. Expressed in rods and cones cells.

タンパク質配列:
MDFGSLETVVANSAFIAARGSFDGSSSQPSRDKKYLAKLKLPPLSKCESLRDSLSLEFESVCLEQPIGKKLFQQFLQSAEKHLPALELWKDIEDYDTADNDLQPQKAQTILAQYLDPQAKLFCSFLDEGIVAKFKEGPVEIQDGLFQPLLQATLAHLGQAPFQEYLGSLYFLRFLQWKWLEAQPMGEDWFLDFRVLGKGGFGEVSACQMKATGKLYACKKLNKKRLKKRKGYQGAMVEKKILMKVHSRFIVSLAYAFETKADLCLVMTIMNGGDIRYHIYNVNEENPGFPEPRALFYTAQIICGLEHLHQRRIVYRDLKPENVLLDNDGNVRISDLGLAVELLDGQSKTKGYAGTPGFMAPELLQGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKHRIISEPVKYPDKFSQASKDFCEALLEKDPEKRLGFRDETCDKLRAHPLFKDLNWRQLEAGMLMPPFIPDSKTVYAKDIQDVGAFSTVKGVAFDKTDTEFFQEFATGNCPIPWQEEMIETGIFGELNVWRSDGQMPDDMKGISGGSSSSSKSGMCLVS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Xenopus
100
Rabbit
100
Horse
88
Bovine
88
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15835 基板として

Site PTM Type Enzyme
S21 Phosphorylation Q15835 (GRK1) , P17612 (PRKACA)
S78 Phosphorylation
K81 Acetylation
K418 Acetylation
K424 Acetylation
K437 Acetylation
S491 Phosphorylation Q15835 (GRK1)
T492 Phosphorylation Q15835 (GRK1)

PTMs - Q15835 酵素として

Substrate Site Source
P08100 (RHO) S334 Uniprot
P08100 (RHO) S338 Uniprot
P08100 (RHO) S343 Uniprot
P41145 (OPRK1) S358 Uniprot
Q15835 (GRK1) S21 Uniprot
Q15835 (GRK1) S491 Uniprot
Q15835 (GRK1) T492 Uniprot
Q5NUL3-2 (FFAR4) T347 Uniprot
Q5NUL3-2 (FFAR4) S350 Uniprot
Q5NUL3-2 (FFAR4) S357 Uniprot

研究背景

機能:

Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity). May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).

PTMs:

Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.

Farnesylation is required for full activity.

細胞の位置付け:

Membrane>Lipid-anchor. Cell projection>Cilium>Photoreceptor outer segment.
Note: Subcellular location is not affected by light or dark conditions.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Retinal-specific. Expressed in rods and cones cells.

サブユニット構造:

Interacts (via N-terminus) with RCVRN (via C-terminus); the interaction is Ca(2+)-dependent (By similarity). Interacts (when prenylated) with PDE6D; this promotes release from membranes (By similarity). May form a complex composed of RHO, GRK1 and RCVRN in a Ca(2+)-dependent manner; RCVRN prevents the interaction between GRK1 and RHO (By similarity).

タンパク質ファミリー:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Sensory system > Phototransduction.

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