Phospho-MLKL (Ser125) Antibody - #AF7419
(2)
(5)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7419, RRID:AB_2843859.
折りたたみ／展開
9130019I15Rik; FLJ34389; hMLKL; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain like pseudokinase; Mixed lineage kinase domain-like protein; Mlkl; MLKL_HUMAN;
免疫原
- Q8NB16 MLKL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQYLPPKCMQEIPQEQIKEIKKEQLSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGICIDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGFELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK
PTMs - Q8NB16 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K40 | Ubiquitination | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| T246 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| K354 | Acetylation | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| T357 | Phosphorylation | Q9Y572 (RIPK3) | Uniprot |
| S358 | Phosphorylation | Q9Y572 (RIPK3) | Uniprot |
| T364 | Phosphorylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| T374 | Phosphorylation | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot |
研究背景
Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function.
Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.
Cytoplasm. Cell membrane.
Note: Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.
Homooligomer. Homotrimer; forms homotrimers on necroptosis induction. Interacts with RIPK3; the interaction is direct. Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5.
The protein kinase domain is catalytically inactive but contains an unusual pseudoactive site with an interaction between Lys-230 and Gln-356 residues. Upon phosphorylation by RIPK3, undergoes an active conformation (By similarity).
The coiled coil region 2 is responsible for homotrimerization.
Belongs to the protein kinase superfamily.
研究領域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
参考文献
Application: WB Species: rat Sample: liver
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