Phospho-DNAJC2/MPP11 (Ser49) Antibody - #AF7434
| 製品: | Phospho-DNAJC2/MPP11 (Ser49) Antibody |
| カタログ: | AF7434 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-DNAJC2/MPP11 (Ser49) |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus |
| 分子量: | 71kDa; 72kD(Calculated). |
| ユニプロット: | Q99543 |
| RRID: | AB_2843874 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7434, RRID:AB_2843874.
折りたたみ／展開
AU020218; DnaJ (Hsp40) homolog subfamily C member 2; DnaJ homolog subfamily C member 2; Dnajc2; DNJC2_HUMAN; M phase phosphoprotein 11; M-phase phosphoprotein 11; MGC105894; MIDA1; MPHOSPH11; MPP11; OTTMUSP00000031226; ZRF1; Zrf2; ZUO1; Zuotin related factor 1; Zuotin related factor 2; Zuotin-related factor 1;
免疫原
- Q99543 DNJC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLLLPSAADGRGTAITHALTSASTLCQVEPVGRWFEAFVKRRNRNASASFQELEDKKELSEESEDEELQLEEFPMLKTLDPKDWKNQDHYAVLGLGHVRYKATQRQIKAAHKAMVLKHHPDKRKAAGEPIKEGDNDYFTCITKAYEMLSDPVKRRAFNSVDPTFDNSVPSKSEAKDNFFEVFTPVFERNSRWSNKKNVPKLGDMNSSFEDVDIFYSFWYNFDSWREFSYLDEEEKEKAECRDERRWIEKQNRATRAQRKKEEMNRIRTLVDNAYSCDPRIKKFKEEEKAKKEAEKKAKAEAKRKEQEAKEKQRQAELEAARLAKEKEEEEVRQQALLAKKEKDIQKKAIKKERQKLRNSCKTWNHFSDNEAERVKMMEEVEKLCDRLELASLQCLNETLTSCTKEVGKAALEKQIEEINEQIRKEKEEAEARMRQASKNTEKSTGGGGNGSKNWSEDDLQLLIKAVNLFPAGTNSRWEVIANYMNIHSSSGVKRTAKDVIGKAKSLQKLDPHQKDDINKKAFDKFKKEHGVVPQADNATPSERFEGPYTDFTPWTTEEQKLLEQALKTYPVNTPERWEKIAEAVPGRTKKDCMKRYKELVEMVKAKKAAQEQVLNASRAKK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99543 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| Y90 | Phosphorylation | Uniprot | |
| Y100 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| Y137 | Phosphorylation | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| T183 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| Y274 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| T539 | Phosphorylation | Uniprot | |
| Y548 | Phosphorylation | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| T573 | Phosphorylation | Uniprot | |
| K606 | Methylation | Uniprot | |
| K607 | Methylation | Uniprot | |
| S617 | Phosphorylation | Uniprot |
研究背景
Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'.
Phosphorylated in M (mitotic) phase.
Nucleus. Cytoplasm>Cytosol.
Widely expressed.
Interacts (via ZRF1-UBD region) with ID1 (By similarity). Component of ribosome-associated complex (RAC), a heterodimer composed of Hsp70/DnaK-type chaperone HSPA14 and Hsp40/DnaJ-type chaperone DNAJC2.
The ZRF1-UBD region specifically recognizes and binds H2AK119ub. The ZRF1-UBD region is also involved in protein-protein interactions with other proteins, suggesting that it may be masked by some regulator, thereby preventing its association with H2AK119ub.
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