Phospho-SHP1 (Tyr536) Antibody - #AF3244

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製品: Phospho-SHP1 (Tyr536) Antibody
カタログ: AF3244
タンパク質の説明: Rabbit polyclonal antibody to Phospho-SHP1 (Tyr536)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 67kDa; 68kD(Calculated).
ユニプロット: P29350
RRID: AB_2834670

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(91%), Chicken(82%)
クローナリティ:
Polyclonal
特異性:
Phospho-SHP1 (Tyr536) Antibody detects endogenous levels of SHP1 only when phosphorylated at Tyrosine 536.
RRID:
AB_2834670
引用形式: Affinity Biosciences Cat# AF3244, RRID:AB_2834670.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

70Z-SHP; EC 3.1.3.48; HCP; HCPH; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP1C; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase non receptor type 6; Protein tyrosine phosphatase SHP1; Protein-tyrosine phosphatase 1C; protein-tyrosine phosphatase SHP 1; Protein-tyrosine phosphatase SHP-1; PTN6_HUMAN; PTP 1C; PTP-1C; PTP1C; Ptpn6; SH PTP 1; SH PTP1; SH-PTP1; SHP 1; SHP 1L; SHP1; SHP1L; tyrosine protein phosphatase non receptor type 6; Tyrosine-protein phosphatase non-receptor type 6;

免疫原

免疫原:
Uniprot:

P29350(PTN6_HUMAN) >>Visit HPA database.

遺伝子(ID):
PTPN6(5777)
発現特異性:
P29350 PTN6_HUMAN:

Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.

タンパク質の説明:
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation.
タンパク質配列:
MVRWFHRDLSGLDAETLLKGRGVHGSFLARPSRKNQGDFSLSVRVGDQVTHIRIQNSGDFYDLYGGEKFATLTELVEYYTQQQGVLQDRDGTIIHLKYPLNCSDPTSERWYHGHMSGGQAETLLQAKGEPWTFLVRESLSQPGDFVLSVLSDQPKAGPGSPLRVTHIKVMCEGGRYTVGGLETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKGKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYIKNQLLGPDENAKTYIASQGCLEATVNDFWQMAWQENSRVIVMTTREVEKGRNKCVPYWPEVGMQRAYGPYSVTNCGEHDTTEYKLRTLQVSPLDNGDLIREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMENISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Chicken
82
Xenopus
70
Zebrafish
63
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29350 基板として

Site PTM Type Enzyme
S10 Phosphorylation
K19 Ubiquitination
S26 Phosphorylation
K34 Ubiquitination
S57 Phosphorylation
Y61 Phosphorylation
Y64 Phosphorylation
K97 Ubiquitination
Y98 Phosphorylation
Y111 Phosphorylation
S140 Phosphorylation
K155 Ubiquitination
K168 Ubiquitination
Y176 Phosphorylation
T177 Phosphorylation
S186 Phosphorylation
T188 Phosphorylation
K195 Ubiquitination
K196 Ubiquitination
Y208 Phosphorylation
Y213 Phosphorylation
Y214 Phosphorylation
K232 Ubiquitination
K241 Ubiquitination
S250 Phosphorylation
K253 Ubiquitination
K257 Ubiquitination
K271 Ubiquitination
Y276 Phosphorylation
K277 Acetylation
K277 Ubiquitination
S285 Phosphorylation
R286 Methylation
S299 Phosphorylation
Y301 Phosphorylation
Y306 Phosphorylation
K308 Ubiquitination
K360 Ubiquitination
Y364 Phosphorylation
Y374 Phosphorylation
Y377 Phosphorylation
S378 Phosphorylation
K391 Ubiquitination
T394 Phosphorylation
S398 Phosphorylation
C453 S-Nitrosylation
K486 Ubiquitination
S496 Phosphorylation
Y509 Phosphorylation
K521 Ubiquitination
K522 Ubiquitination
S528 Phosphorylation
K530 Ubiquitination
S534 Phosphorylation
Y536 Phosphorylation P06213 (INSR) , P00519 (ABL1) , P12931 (SRC) , A0A173G4P4 (Abl fusion) , P06239 (LCK) , P07948 (LYN)
T540 Phosphorylation
Y541 Phosphorylation
K546 Ubiquitination
S553 Phosphorylation
T555 Phosphorylation
S556 Phosphorylation
S557 Phosphorylation
K560 Ubiquitination
Y564 Phosphorylation A0A173G4P4 (Abl fusion) , P00519 (ABL1) , P07948 (LYN) , P06239 (LCK) , P12931 (SRC)
K570 Ubiquitination
S582 Phosphorylation
S588 Phosphorylation
S591 Phosphorylation P17252 (PRKCA) , P24941 (CDK2)

研究背景

機能:

Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.

PTMs:

Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation (By similarity). Phosphorylation at Tyr-564 enhances phosphatase activity.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). Shuttles between the cytoplasm and nucleus via its association with PDPK1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.

サブユニット構造:

Monomer. Interacts with MTUS1 (By similarity). Interacts with MILR1 (tyrosine-phosphorylated) (By similarity). Interacts with KIT (By similarity). Interacts with SIRPA/PTPNS1. Interacts with LILRB1 and LILRB2. Interacts with FCRL2 and FCRL4. Interacts with FCRL3 and FCRL6 (tyrosine phosphorylated form). Interacts with CD84. Interacts with CD300LF. Interacts with CDK2. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1. Interacts with CEACAM1 (via cytoplasmic domain); this interaction depends on the monomer/dimer equilibrium and is phosphorylation-dependent (By similarity). Interacts with MPIG6B (via ITIM motif). Interacts with moesin/MSN.

タンパク質ファミリー:

The N-terminal SH2 domain functions as an auto-inhibitory domain, blocking the catalytic domain in the ligand-free close conformation.

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

参考文献

1). Effects of Poria cocos extract on metabolic dysfunction-associated fatty liver disease via the FXR/PPARα-SREBPs pathway. Frontiers in Pharmacology, 2022 (PubMed: 36278226) [IF=5.6]

Application: WB    Species: Rat    Sample:

FIGURE 5 EPC regulated the lipid metabolism-related genes and proteins inMAFLD rats. (A) mRNA abundances of FASN. (B) mRNA abundances of PPARα (PPARΑ). (C) mRNA abundances of PPARγ (PPARG). (D) mRNA abundances of RXRα. (E) mRNA abundances of CYP19A1. (F) mRNA abundances of NR3C1. (G) mRNA abundances of SREBP-1c. (H) mRNA abundances of HMGCR. (I) mRNA abundances of SCD. n = 6; (J) Relative expression of protein SCD. (K) Relative expression of protein PPARα. (L) Relative expression of protein FASN. (M) Relative expression of protein p-JNK. (N) Relative expression of protein p-NF-κB. (O) Relative expression of protein CYP19A1. (P) Relative expression of protein NR3C1. (Q-R) Representative immunoblotting images of β-actin, SCD, PPARα, FASN,CYP19A1, NR3C1, JNK, p-JNK, NF-κB, pNF-κB; n = 4, data are presented as mean ± SEM. One-way analysis of variance (ANOVA) was conducted for the group comparison. *p < 0.05, **p < 0.01, ***p < 0.001 vs MOD group. FASN, fatty acid synthase; PPARα/γ, peroxisome proliferator-activated receptor alpha/gamma; RXRa, retinoic acid receptor alpha; SREBP-1c, sterol regulatory element binding protein 1c; HMGCR, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; SCD, Stearoyl-CoA desaturase; p-JNK, phosphorylation (p) -stress-activated protein kinase JNK; NF-κB, nuclear factor kappa B.
2). SHP1 tyrosine phosphatase gets involved in host defense against Streptococcus agalactiae infection and BCR signaling pathway in Nile tilapia (Oreochromis niloticus). FISH & SHELLFISH IMMUNOLOGY, 2020 (PubMed: 32109611) [IF=4.7]

Application: WB    Species: fish    Sample: splenic leukocytes

Fig. 7. |The detection of tyrosine phosphorylation level (A and B) and protein expression (C) of OnSHP1 by Western blotting after the splenic leukocytes were incubated with PTP inhibitor II in vitro for 1 h. Phospho-OnSHP1 (Y535) (A) and phospho-SHP1 (Y557) (B) were detected by rabbit anti-phospho-SHP1 (Tyr536) and phospho-SHP1 (Tyr564) antibody, respectively. The protein expression level of OnSHP1 was detected with anti-mouse SHP1 Ab (C).

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