Phospho-KIF5B (Ser154) Antibody - #AF7447

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製品: Phospho-KIF5B (Ser154) Antibody
カタログ: AF7447
タンパク質の説明: Rabbit polyclonal antibody to Phospho-KIF5B (Ser154)
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 110kDa; 110kD(Calculated).
ユニプロット: P33176
RRID: AB_2843887

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-KIF5B (Ser154) Antibody detects endogenous levels of KIF5B only when phosphorylated at Ser154.
RRID:
AB_2843887
引用形式: Affinity Biosciences Cat# AF7447, RRID:AB_2843887.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Conventional kinesin heavy chain; KIF 5B; KIF5B; Kinesin 1; kinesin 1 (110-120kD); Kinesin 1 heavy chain; Kinesin family member 5B; Kinesin heavy chain; kinesin, heavy chain, ubiquitous; Kinesin-1 heavy chain; Kinesin1; KINH; KINH_HUMAN; KNS 1; KNS; KNS1; Ubiquitous kinesin heavy chain; UKHC;

免疫原

免疫原:
Uniprot:

P33176(KINH_HUMAN) >>Visit HPA database.

遺伝子(ID):
KIF5B(3799)
タンパク質配列:
MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEIDSDDTGGSAAQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKNMARRGHSAQIAKPIRPGQHPAASPTHPSAIRGGGAFVQNSQPVAVRGGGGKQV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P33176 基板として

Site PTM Type Enzyme
A2 Acetylation
K10 Acetylation
K10 Ubiquitination
S21 Phosphorylation
K28 Acetylation
S43 Phosphorylation
K44 Ubiquitination
S55 Phosphorylation
C65 S-Nitrosylation
K67 Ubiquitination
K150 Ubiquitination
S154 Phosphorylation
K159 Ubiquitination
K166 Acetylation
S175 Phosphorylation
S206 Phosphorylation
K222 Ubiquitination
K226 Ubiquitination
K240 Ubiquitination
K252 Ubiquitination
Y277 Phosphorylation
R321 Methylation
K346 Acetylation
K382 Ubiquitination
K384 Ubiquitination
K394 Ubiquitination
T397 Phosphorylation
T399 Phosphorylation
K402 Ubiquitination
T405 Phosphorylation
T414 Phosphorylation
K420 Ubiquitination
K427 Ubiquitination
K430 Ubiquitination
K448 Ubiquitination
T451 Phosphorylation
T463 Phosphorylation
R464 Methylation
K505 Ubiquitination
K514 Ubiquitination
Y516 Phosphorylation
K526 Ubiquitination
K539 Ubiquitination
K549 Acetylation
K560 Acetylation
Y593 Phosphorylation
S599 Phosphorylation
K602 Acetylation
K609 Acetylation
K609 Ubiquitination
C632 S-Nitrosylation
K644 Ubiquitination
S645 Phosphorylation
Y649 Phosphorylation
K656 Ubiquitination
K657 Ubiquitination
S663 Phosphorylation
K679 Ubiquitination
K690 Ubiquitination
K698 Ubiquitination
K761 Acetylation
K764 Acetylation
K764 Ubiquitination
K783 Ubiquitination
K791 Ubiquitination
K801 Ubiquitination
S814 Phosphorylation
S819 Phosphorylation
S825 Phosphorylation
S833 Phosphorylation
K844 Ubiquitination
K847 Ubiquitination
R901 Methylation
S917 Phosphorylation
K922 Ubiquitination
S933 Phosphorylation
T935 Phosphorylation
S938 Phosphorylation
S950 Phosphorylation
R956 Methylation

研究背景

機能:

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).

細胞の位置付け:

Cytoplasm>Cytoskeleton.
Note: Uniformly distributed between soma and neurites in hippocampal neurons.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 and PPP1R42 (By similarity). Interacts with SYBU. Interacts with JAKMIP1. Interacts with PLEKHM2. Interacts with ECPAS. Interacts with ZFYVE27 (By similarity). Found in a complex with OGT, RHOT1, RHOT2 and TRAK1. Interacts with APP (via cytoplasmic domain).

タンパク質ファミリー:

Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

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