N-WASP Antibody - #AF7532
製品: | N-WASP Antibody |
カタログ: | AF7532 |
タンパク質の説明: | Rabbit polyclonal antibody to N-WASP |
アプリケーション: | WB |
反応性: | Human, Mouse |
予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 54kDa, 70 kDa; 55kD(Calculated). |
ユニプロット: | O00401 |
RRID: | AB_2843896 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7532, RRID:AB_2843896.
折りたたみ/展開
DKFZp779G0847; MGC48327; N-WASP; Neural Wiskott Aldrich syndrome protein; Neural Wiskott-Aldrich syndrome protein; NWASP; Wasl; WASL_HUMAN; WASPB; Wiskott Aldrich syndrome gene like; Wiskott Aldrich syndrome gene like protein; Wiskott Aldrich syndrome like; WiskottAldrich syndrome like;
免疫原
- O00401 WASL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHNSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAVPPPPPNRMYPPPPPALPSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVADGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDEDDEEDFEDDDEWED
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O00401 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Acetylation | Uniprot | |
T23 | Phosphorylation | Uniprot | |
S58 | Phosphorylation | Uniprot | |
Y95 | Phosphorylation | Uniprot | |
Y100 | Phosphorylation | Uniprot | |
S102 | Phosphorylation | Uniprot | |
T135 | Phosphorylation | Uniprot | |
Y175 | Phosphorylation | P00519 (ABL1) | Uniprot |
S183 | Phosphorylation | Uniprot | |
K186 | Ubiquitination | Uniprot | |
T199 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
T205 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
S242 | Phosphorylation | Uniprot | |
K253 | Ubiquitination | Uniprot | |
Y256 | Phosphorylation | P42684 (ABL2) , P00519 (ABL1) , Q05397 (PTK2) | Uniprot |
K261 | Ubiquitination | Uniprot | |
T262 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
R274 | Methylation | Uniprot | |
R284 | Methylation | Uniprot | |
R305 | Methylation | Uniprot | |
R307 | Methylation | Uniprot | |
R329 | Methylation | Uniprot | |
S426 | Phosphorylation | Uniprot | |
S430 | Phosphorylation | Uniprot | |
S456 | Phosphorylation | Uniprot | |
T457 | Phosphorylation | Uniprot | |
T460 | Phosphorylation | Uniprot | |
T464 | Phosphorylation | Uniprot | |
S484 | Phosphorylation | Uniprot | |
S485 | Phosphorylation | Uniprot |
研究背景
Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (By similarity). Plays a role in dendrite spine morphogenesis (By similarity). Decreasing levels of DNMBP (using antisense RNA) alters apical junction morphology in cultured enterocytes, junctions curve instead of being nearly linear.
Phosphorylation at Ser-242, Tyr-256, Ser-484 and Ser-485 enhances actin polymerization activity.
Cytoplasm>Cytoskeleton. Nucleus. Cytoplasm.
Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated (By similarity). Exported from the nucleus by an nuclear export signal (NES)-dependent mechanism to the cytoplasm (By similarity).
Binds actin and the Arp2/3 complex. Interacts with CDC42. Interacts with FCHSD1 (By similarity). Interacts with FCHSD2. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with SNX9 (By similarity). Interacts with the WW domains of PRPF40A/FBP11 (By similarity). Interacts with PTK2/FAK1 (By similarity). Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Interacts with NOSTRIN. Binds to TNK2. Interacts with SNX33. Interacts with NONO (via second RRM domain); the interaction is direct. Component of a multiprotein complex with NONO and SFPQ; associates with the complex via direct interaction with NONO.
(Microbial infection) Interacts with E.coli effector protein EspF(U). Identified in a complex containing at least WASL, BAIAP2L1 and E.coli EspF(U).
(Microbial infection) Interacts with Shigella flexneri protein IcsA. The interaction with IcsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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