BCL11A Antibody - #AF7561

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製品: BCL11A Antibody
カタログ: AF7561
タンパク質の説明: Rabbit polyclonal antibody to BCL11A
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 90~150kDa; 91kD(Calculated).
ユニプロット: Q9H165
RRID: AB_2843925

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
BCL11A Antibody detects endogenous levels of total BCL11A.
RRID:
AB_2843925
引用形式: Affinity Biosciences Cat# AF7561, RRID:AB_2843925.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

2810047E18Rik; B cell CLL/lymphoma 11A (zinc finger protein); B cell CLL/lymphoma 11A (zinc finger protein) isoform 2; B-cell CLL/lymphoma 11A; B-cell lymphoma/leukemia 11A; BC11A_HUMAN; BCL-11A; BCL11A; BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1; BCL11A L; BCL11A S; BCL11A XL; BCL11a-M; BCL11AL; BCL11AS; BCL11AXL; C2H2 type zinc finger protein; COUP TF interacting protein 1; COUP-TF-interacting protein 1; CTIP1; CTIP1, mouse, homolog of; D930021L15Rik; Ecotropic viral integration site 9; Ecotropic viral integration site 9 homolog; Ecotropic viral integration site 9 protein; Ecotropic viral integration site 9 protein homolog; EVI-9; Evi9; Evi9, mouse, homolog of; FLJ10173; FLJ34997; HBFQTL5; KIAA1809; mKIAA1809; OTTHUMP00000159788; OTTHUMP00000159789; OTTHUMP00000201250; OTTHUMP00000202084; Zinc finger protein 856; ZNF856;

免疫原

免疫原:
Uniprot:

Q9H165(BC11A_HUMAN) >>Visit HPA database.

遺伝子(ID):
BCL11A(53335)
発現特異性:
Q9H165 BC11A_HUMAN:

Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.

タンパク質配列:
MSRRKQGKPQHLSKREFSPEPLEAILTDDEPDHGPLGAPEGDHDLLTCGQCQMNFPLGDILIFIEHKRKQCNGSLCLEKAVDKPPSPSPIEMKKASNPVEVGIQVTPEDDDCLSTSSRGICPKQEHIADKLLHWRGLSSPRSAHGALIPTPGMSAEYAPQGICKDEPSSYTCTTCKQPFTSAWFLLQHAQNTHGLRIYLESEHGSPLTPRVGIPSGLGAECPSQPPLHGIHIADNNPFNLLRIPGSVSREASGLAEGRFPPTPPLFSPPPRHHLDPHRIERLGAEEMALATHHPSAFDRVLRLNPMAMEPPAMDFSRRLRELAGNTSSPPLSPGRPSPMQRLLQPFQPGSKPPFLATPPLPPLQSAPPPSQPPVKSKSCEFCGKTFKFQSNLVVHRRSHTGEKPYKCNLCDHACTQASKLKRHMKTHMHKSSPMTVKSDDGLSTASSPEPGTSDLVGSASSALKSVVAKFKSENDPNLIPENGDEEEEEDDEEEEEEEEEEEEELTESERVDYGFGLSLEAARHHENSSRGAVVGVGDESRALPDVMQGMVLSSMQHFSEAFHQVLGEKHKRGHLAEAEGHRDTCDEDSVAGESDRIDDGTVNGRGCSPGESASGGLSKKLLLGSPSSLSPFSKRIKLEKEFDLPPAAMPNTENVYSQWLAGYAASRQLKDPFLSFGDSRQSPFASSSEHSSENGSLRFSTPPGELDGGISGRSGTGSGGSTPHISGPGPGRPSSKEGRRSDTCEYCGKVFKNCSNLTVHRRSHTGERPYKCELCNYACAQSSKLTRHMKTHGQVGKDVYKCEICKMPFSVYSTLEKHMKKWHSDRVLNNDIKTE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H165 基板として

Site PTM Type Enzyme
S86 Phosphorylation
S88 Phosphorylation
K123 Ubiquitination
K130 Ubiquitination
T150 Phosphorylation
Y198 Phosphorylation
S201 Phosphorylation
S205 Phosphorylation
T208 Phosphorylation
T262 Phosphorylation
S267 Phosphorylation
S328 Phosphorylation
S332 Phosphorylation
T357 Phosphorylation
K377 Ubiquitination
K384 Acetylation
K384 Ubiquitination
T400 Phosphorylation
T426 Phosphorylation
S432 Phosphorylation
T435 Phosphorylation
S446 Phosphorylation
S447 Phosphorylation
T584 Phosphorylation
S589 Phosphorylation
S594 Phosphorylation
S608 Phosphorylation
S612 Phosphorylation
S618 Phosphorylation
K619 Ubiquitination
K620 Ubiquitination
S625 Phosphorylation
S627 Phosphorylation
S628 Phosphorylation
S630 Phosphorylation
S633 Phosphorylation
K634 Ubiquitination
K670 Ubiquitination
T701 Phosphorylation
S714 Phosphorylation
S718 Phosphorylation
S721 Phosphorylation
T722 Phosphorylation
K736 Ubiquitination
S741 Phosphorylation
T743 Phosphorylation
K749 Ubiquitination
K752 Ubiquitination
S755 Phosphorylation
T765 Phosphorylation
K771 Ubiquitination
K784 Ubiquitination

研究背景

機能:

Transcription factor associated with the BAF SWI/SNF chromatin remodeling complex (By similarity). Repressor of fetal hemoglobin (HbF) level. Involved in brain development. May play a role in hematopoiesis. Essential factor in lymphopoiesis required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1 (By similarity).

PTMs:

Sumoylated with SUMO1.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles (By similarity).

Cytoplasm. Nucleus.
Note: Predominantly localized in the nucleus in nuclear paraspeckles.

Cytoplasm. Nucleus.
Note: Predominantly localized in the cytoplasm in the absence of interaction with isoform 1 and isoform 2. In presence of isoform 1 or isoform 2, translocates from the cytoplasm into nuclear paraspeckles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.

サブユニット構造:

Interacts with TFCOUP1, PIAS3, ARP1 and EAR2 (By similarity). Isoform 1, isoform 2 and isoform 3 form homodimers and heterodimers. Isoform 2 interacts with TBR1.

タンパク質ファミリー:

The N-terminus is involved in protein dimerization and in transactivation of transcription.

The C-terminus of isoform 2 is necessary for isoform 2 interaction with TBR1.

参考文献

1). The antioxidant Glycitin protects against intervertebral disc degeneration through antagonizing inflammation and oxidative stress in nucleus pulposus cells. Aging (Albany NY), 2023 (PubMed: 38019477)

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