osterix Antibody - #AF7580

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製品: osterix Antibody
カタログ: AF7580
タンパク質の説明: Rabbit polyclonal antibody to osterix
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus
分子量: 46kDa, 37 kDa; 45kD(Calculated).
ユニプロット: Q8TDD2
RRID: AB_2843944

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MS Report
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説明書
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
osterix Antibody detects endogenous levels of total osterix.
RRID:
AB_2843944
引用形式: Affinity Biosciences Cat# AF7580, RRID:AB_2843944.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

MGC126598; Osterix; Osx; Sp 7; SP7; Sp7 transcription factor; SP7_HUMAN; Transcription factor Sp7; Zinc finger protein osterix;

免疫原

免疫原:
Uniprot:

Q8TDD2(SP7_HUMAN) >>Visit HPA database.

遺伝子(ID):
SP7(121340)
発現特異性:
Q8TDD2 SP7_HUMAN:

Restricted to bone-derived cell.

タンパク質配列:
MASSLLEEEVHYGSSPLAMLTAACSKFGGSSPLRDSTTLGKAGTKKPYSVGSDLSASKTMGDAYPAPFTSTNGLLSPAGSPPAPTSGYANDYPPFSHSFPGPTGTQDPGLLVPKGHSSSDCLPSVYTSLDMTHPYGSWYKAGIHAGISPGPGNTPTPWWDMHPGGNWLGGGQGQGDGLQGTLPTGPAQPPLNPQLPTYPSDFAPLNPAPYPAPHLLQPGPQHVLPQDVYKPKAVGNSGQLEGSGGAKPPRGASTGGSGGYGGSGAGRSSCDCPNCQELERLGAAAAGLRKKPIHSCHIPGCGKVYGKASHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELERHVRTHTREKKFTCLLCSKRFTRSDHLSKHQRTHGEPGPGPPPSGPKELGEGRSTGEEEASQTPRPSASPATPEKAPGGSPEQSNLLEI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TDD2 基板として

Site PTM Type Enzyme
S30 Phosphorylation
S31 Phosphorylation
T37 Phosphorylation
T38 Phosphorylation
K41 Ubiquitination
S49 Phosphorylation
S52 Phosphorylation
K58 Ubiquitination
S76 Phosphorylation Q16539 (MAPK14)
S80 Phosphorylation Q16539 (MAPK14)
K230 Ubiquitination
S237 Phosphorylation
K307 Acetylation
K312 Acetylation
T319 Phosphorylation
S338 Phosphorylation
T364 Phosphorylation
S366 Phosphorylation
S370 Phosphorylation
S396 Phosphorylation
T397 Phosphorylation
S422 Phosphorylation

研究背景

機能:

Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (By similarity).

Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences.

PTMs:

Ubiquitination at leads to proteasomal degradation. SP7 is a short-live protein with an endogenous half-life of approximately 12 hours.

Propionylated. Depropionylation at Lys-371 by SIRT7 activates transcription factor activity and positively regulates bone formation by osteoblasts.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Restricted to bone-derived cell.

サブユニット構造:

Interacts with RIOX1; the interaction is direct and inhibits transcription activator activity.

タンパク質ファミリー:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the Sp1 C2H2-type zinc-finger protein family.

参考文献

1). MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm. Cell Death & Differentiation, 2021 (PubMed: 33664480) [IF=12.4]

Application: WB    Species: Human    Sample: MACF1-KD cells

Fig. 1 MACF1 knockdown inhibits osteoblast differentiation and alters osteoblast differentiation transcriptome dynamics. A ALP activity of Control and MACF1-KD cells was detected by ALP staining. B The expression of ALP and Runx2 examined by real-time PCR during osteoblastic differentiation in Control and MACF1-KD cells. Data represent mean value ± SD (n = 3). (**P 
2). Metformin protects against vascular calcification through the selective degradation of Runx2 by the p62 autophagy receptor. Journal of Cellular Physiology, 2022 (PubMed: 36166694) [IF=5.6]

Application: WB    Species: Mouse    Sample: VSMCs

FIGURE 1Metformin activates AMPK and calcification inVSMCs. VSMCs were cultured with high phosphate (3 mM Pi) and metformin (met)for 7 days. (a) Calcium content (mg/mg protein) of cells treated with metformin (0.5−1.5 mM), (n= 3). (b) Representative alizarin red images ofcells treated with metformin (1 mM). (c) Representative immunoblots (d) immunoblot quantification showing the effect of metformin (1 mM) onexpression of osteogenic markers osterix, osteocalcin, and bone sialoprotein compared withβ‐actin (n= 3). (e) Representative immunoblots and(f) immunoblot quantification showing the effect of metformin (1 mM) on the phosphorylation of AMPK compared with total AMPK (n= 4).AMPK, adenosine monophosphate‐activated protein kinase; VSMCs, vascular smooth muscle cells.PHADWALET AL.|4307

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