PAX7 Antibody - #AF7584

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製品: PAX7 Antibody
カタログ: AF7584
タンパク質の説明: Rabbit polyclonal antibody to PAX7
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat, Sheep
予測: Pig, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 57kDa; 55kD(Calculated).
ユニプロット: P23759
RRID: AB_2843948

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Sheep
予測:
Pig(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(90%)
クローナリティ:
Polyclonal
特異性:
PAX7 Antibody detects endogenous levels of total PAX7.
RRID:
AB_2843948
引用形式: Affinity Biosciences Cat# AF7584, RRID:AB_2843948.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FLJ37460; HUP1; OTTHUMP00000002534; Paired box 7; Paired box gene 7; Paired box homeotic gene 7; Paired box protein Pax-7; Paired domain gene 7; Paired domain gene HuP1; Pax7; PAX7 transcriptional factor; PAX7/FKHR fusion gene, included; PAX7_HUMAN; PAX7B; RGD1564360; RMS2;

免疫原

免疫原:
Uniprot:

P23759(PAX7_HUMAN) >>Visit HPA database.

遺伝子(ID):
PAX7(5081)
タンパク質配列:
MAALPGTVPRMMRPAPGQNYPRTGFPLEVSTPLGQGRVNQLGGVFINGRPLPNHIRHKIVEMAHHGIRPCVISRQLRVSHGCVSKILCRYQETGSIRPGAIGGSKPRQVATPDVEKKIEEYKRENPGMFSWEIRDRLLKDGHCDRSTVPSGLVSSISRVLRIKFGKKEEEDEADKKEDDGEKKAKHSIDGILGDKGNRLDEGSDVESEPDLPLKRKQRRSRTTFTAEQLEELEKAFERTHYPDIYTREELAQRTKLTEARVQVWFSNRRARWRKQAGANQLAAFNHLLPGGFPPTGMPTLPPYQLPDSTYPTTTISQDGGSTVHRPQPLPPSTMHQGGLAAAAAAADTSSAYGARHSFSSYSDSFMNPAAPSNHMNPVSNGLSPQVMSILGNPSAVPPQPQADFSISPLHGGLDSATSISASCSQRADSIKPGDSLPTSQAYCPPTYSTTGYSVDPVAGYQYGQYGQTAVDYLAKNVSLSTQRRMKLGEHSAVLGLLPVETGQAY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
90
Horse
0
Bovine
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23759 基板として

Site PTM Type Enzyme
Y121 Phosphorylation
T147 Phosphorylation
K176 Acetylation
S203 Phosphorylation
S207 Phosphorylation

研究背景

機能:

Transcription factor playing a role in myogenesis through regulation of muscle precursor cells proliferation.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Can bind to DNA as a heterodimer with PAX3. Interacts with PAXBP1; the interaction links PAX7 to a WDR5-containing histone methyltransferase complex (By similarity). Interacts with DAXX.

タンパク質ファミリー:

Belongs to the paired homeobox family.

研究領域

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

参考文献

1). Kcnma1 is involved in mitochondrial homeostasis in diabetes-related skeletal muscle atrophy. The FASEB Journal, 2023 (PubMed: 36929614) [IF=4.4]
2). Deletion of phosphatidylserine flippase β-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration. ZOOLOGICAL RESEARCH, 2021 (PubMed: 34472226) [IF=4.0]

Application: IF/ICC    Species: Mice    Sample: satellite cells (SCs)

Figure 2 Generation of Tmem30a conditional knockout (cKO) mice A: Breeding strategy of cKO mice by crossing Tmem30aloxP/loxP with Pax7CreER mice. B: Genotyping of mice by PCR and agarose gel electrophoresis. C: Axis of tamoxifen intraperitoneal injection, BaCl2 injection, and TA muscle harvesting in mice. D: In 7 dpi cKO mice, expression of Pax7CreER was monitored by ROSA-tdTomato reporter (red). SCs were labeled with Pax7 antibody (green). Nuclei were counterstained with DAPI (blue). Scale bar: 50 μm. E: TMEM30A protein from 7 dpi WT and cKO mice was labeled with TMEM30A antibody (green) and DAPI (blue). Scale bar: 50 μm. F, G: Immunoblot analysis of TMEM30A protein expression in 7 dpi WT and cKO mice. Relative protein band density was calculated by ImageJ. Data are mean±SEM. n=3 in each group. Significance was calculated by two-tailed Student’s t-test. *: P<0.05.
3). Bi-phasic effect of gelatin in myogenesis and skeletal muscle regeneration. Disease Models & Mechanisms, 2021 (PubMed: 34821368) [IF=4.0]

Application: IF/ICC    Species: Mouse    Sample: SCs

Fig. 2.Bi-phasic effect of gelatin in activating quiescent satellite cells (SCs) and myogenesis during muscle regeneration. (A) Immunohistochemical (IHC) analysis of Pax7+ SCs (green) in TA muscle stained by laminin (red) at 7 D.P.I. Arrows point to Pax7+ nuclei. Scale bar: 50 μm. (B) The percentage of myofibers containing Pax7+ SCs (n=6). (C) IHC analysis of MyoG+ cells (green) in damaged TA muscle stained by laminin (red) at 7 D.P.I. Arrows point to MyoG+ nuclei. Scale bar: 50 μm. (D) The percentage of myofibers containing MyoG+ cells (n=6). (E,F) Western blots (E) and quantification (F) of MyoD and MyoG in damaged TA muscles at 14 D.P.I. (n=3). GAPDH is used as a loading control. Significance was determined by unpaired two-tailed Student's t-test with Welch's correction.
4). Single-cell RNA transcriptome uncovers distinct developmental trajectories in the embryonic skeletal muscle of Daheng broiler and Tibetan chicken. BMC genomics, 2025 (PubMed: 39994525) [IF=3.5]
5). N-Acetylcysteine assists muscle development in offspring of mice subjected to maternal heat stress during pregnancy. Journal of the science of food and agriculture, 2024 (PubMed: 38828636) [IF=3.3]
6). N-acetylcysteine stimulates the proliferation and differentiation in heat-stressed skeletal muscle cells. Journal of thermal biology, 2024 (PubMed: 39182421) [IF=2.9]
7). The mechanism of Megalobrama amblycephala muscle injury repair based on RNA-seq. Gene, 2022 (PubMed: 35395368) [IF=2.6]

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