PKC beta 1 Antibody - #AF7691
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7691, RRID:AB_2844055.
折りたたみ／展開
KPCB_HUMAN; PKC beta; PKC-B; PKC-beta; PKCB; Prkcb; PRKCB I; PRKCB II; PRKCB1; PRKCB2; Protein kinase C beta 1; Protein kinase C beta 2; Protein kinase C beta; Protein kinase C beta type; protein kinase C, beta 1 polypeptide;
免疫原
- P05771 KPCB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDRDVLIVLVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKASVDGWFKLLSQEEGEYFNVPVPPEGSEANEELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P05771 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| T17 | Phosphorylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| T40 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| Y123 | Phosphorylation | Uniprot | |
| T134 | Phosphorylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| Y195 | Phosphorylation | Uniprot | |
| K197 | Acetylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| T226 | Phosphorylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| T314 | Phosphorylation | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| T364 | Phosphorylation | Uniprot | |
| Y368 | Phosphorylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| K489 | Ubiquitination | Uniprot | |
| T497 | Phosphorylation | Uniprot | |
| T498 | Phosphorylation | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| T500 | Phosphorylation | P53004 (BLVRA) , O15530 (PDPK1) | Uniprot |
| T504 | Phosphorylation | Uniprot | |
| Y507 | Phosphorylation | Uniprot | |
| Y515 | Phosphorylation | Uniprot | |
| K566 | Ubiquitination | Uniprot | |
| K595 | Ubiquitination | Uniprot | |
| T642 | Phosphorylation | P05771 (PRKCB) | Uniprot |
| T644 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | P05771 (PRKCB) | Uniprot |
| Y662 | Phosphorylation | P43405 (SYK) | Uniprot |
PTMs - P05771 酵素として
| Substrate | Site | Source |
|---|---|---|
| O15350 (TP73) | S388 | Uniprot |
| O15519 (CFLAR) | S193 | Uniprot |
| O95786 (DDX58) | S8 | Uniprot |
| O95786 (DDX58) | T170 | Uniprot |
| P04004 (VTN) | S381 | Uniprot |
| P04049 (RAF1) | S497 | Uniprot |
| P04049 (RAF1) | S619 | Uniprot |
| P04083 (ANXA1) | S27 | Uniprot |
| P04083 (ANXA1) | S28 | Uniprot |
| P05107 (ITGB2) | S745 | Uniprot |
| P05107 (ITGB2) | T758 | Uniprot |
| P05771-2 (PRKCB) | S16 | Uniprot |
| P05771-2 (PRKCB) | T17 | Uniprot |
| P05771-1 (PRKCB) | T635 | Uniprot |
| P05771 (PRKCB) | T642 | Uniprot |
| P05771-2 (PRKCB) | S660 | Uniprot |
| P05771 (PRKCB) | S661 | Uniprot |
| P06127 (CD5) | T434 | Uniprot |
| P06127 (CD5) | T436 | Uniprot |
| P06400 (RB1) | S249 | Uniprot |
| P06400 (RB1) | T252 | Uniprot |
| P06400 (RB1) | S780 | Uniprot |
| P06400 (RB1) | S795 | Uniprot |
| P06400 (RB1) | T821 | Uniprot |
| P06730-1 (EIF4E) | S209 | Uniprot |
| P07355-1 (ANXA2) | S2 | Uniprot |
| P07355-2 (ANXA2) | S20 | Uniprot |
| P07355 (ANXA2) | S26 | Uniprot |
| P10636-8 (MAPT) | S258 | Uniprot |
| P10636-8 (MAPT) | S293 | Uniprot |
| P10636-8 (MAPT) | S324 | Uniprot |
| P10636-8 (MAPT) | S352 | Uniprot |
| P11171-4 (EPB41) | S312 | Uniprot |
| P11388 (TOP2A) | S29 | Uniprot |
| P11473 (VDR) | S51 | Uniprot |
| P14598 (NCF1) | S303 | Uniprot |
| P14598 (NCF1) | S304 | Uniprot |
| P14598 (NCF1) | S315 | Uniprot |
| P14598 (NCF1) | S320 | Uniprot |
| P14598 (NCF1) | S328 | Uniprot |
| P14598 (NCF1) | S359 | Uniprot |
| P14598 (NCF1) | S370 | Uniprot |
| P14598 (NCF1) | S379 | Uniprot |
| P14679 (TYR) | S523 | Uniprot |
| P14679 (TYR) | S527 | Uniprot |
| P15336 (ATF2) | S121 | Uniprot |
| P16949 (STMN1) | S25 | Uniprot |
| P16949 (STMN1) | S38 | Uniprot |
| P17302 (GJA1) | S368 | Uniprot |
| P17677-1 (GAP43) | S41 | Uniprot |
| P19429 (TNNI3) | S42 | Uniprot |
| P19429 (TNNI3) | S44 | Uniprot |
| P19429 (TNNI3) | T143 | Uniprot |
| P19484 (TFEB) | S462 | Uniprot |
| P19484 (TFEB) | S463 | Uniprot |
| P19484 (TFEB) | S467 | Uniprot |
| P19484 (TFEB) | S469 | Uniprot |
| P20700 (LMNB1) | S395 | Uniprot |
| P20700 (LMNB1) | S405 | Uniprot |
| P21730 (C5AR1) | S334 | Uniprot |
| P26010-1 (ITGB7) | T783 | Uniprot |
| P28329-3 (CHAT) | T255 | Uniprot |
| P28329-3 (CHAT) | S346 | Uniprot |
| P28329-3 (CHAT) | S347 | Uniprot |
| P28329 (CHAT) | T373 | Uniprot |
| P28329-3 (CHAT) | S440 | Uniprot |
| P28329 (CHAT) | S464 | Uniprot |
| P28329 (CHAT) | S465 | Uniprot |
| P28329-3 (CHAT) | S476 | Uniprot |
| P28329 (CHAT) | S558 | Uniprot |
| P28329 (CHAT) | S594 | Uniprot |
| P28799 (GRN) | S81 | Uniprot |
| P29353 (SHC1) | S36 | Uniprot |
| P29474 (NOS3) | T495 | Uniprot |
| P29966 (MARCKS) | S159 | Uniprot |
| P29966 (MARCKS) | S163 | Uniprot |
| P29966 (MARCKS) | S170 | Uniprot |
| P30086 (PEBP1) | S153 | Uniprot |
| P31749 (AKT1) | T308 | Uniprot |
| P31749 (AKT1) | S473 | Uniprot |
| P34741 (SDC2) | S187 | Uniprot |
| P34741 (SDC2) | S188 | Uniprot |
| P35548 (MSX2) | T135 | Uniprot |
| P35548 (MSX2) | T141 | Uniprot |
| P35568 (IRS1) | S323 | Uniprot |
| P35568 (IRS1) | S441 | Uniprot |
| P35637 (FUS) | S257 | Uniprot |
| P41180 (CASR) | T888 | Uniprot |
| P41594-2 (GRM5) | S840 | Uniprot |
| P43629 (KIR3DL1) | S415 | Uniprot |
| P48736 (PIK3CG) | S582 | Uniprot |
| P49768 (PSEN1) | S346 | Uniprot |
| P49840 (GSK3A) | S21 | Uniprot |
| P49841 (GSK3B) | S9 | Uniprot |
| P55042 (RRAD) | S214 | Uniprot |
| P55042 (RRAD) | S257 | Uniprot |
| P55042 (RRAD) | S290 | Uniprot |
| P55042 (RRAD) | S299 | Uniprot |
| P56537-2 (EIF6) | T215 | Uniprot |
| P56537-1 (EIF6) | T234 | Uniprot |
| P56537 (EIF6) | S235 | Uniprot |
| P61764-1 (STXBP1) | S306 | Uniprot |
| P61764-2 (STXBP1) | S313 | Uniprot |
| P62491 (RAB11A) | S177 | Uniprot |
| P98082 (DAB2) | S24 | Uniprot |
| Q01844 (EWSR1) | S266 | Uniprot |
| Q03721 (KCNC4) | S8 | Uniprot |
| Q03721 (KCNC4) | S9 | Uniprot |
| Q03721 (KCNC4) | S15 | Uniprot |
| Q03721 (KCNC4) | S21 | Uniprot |
| Q05639 (EEF1A2) | S53 | Uniprot |
| Q06124-2 (PTPN11) | S576 | Uniprot |
| Q06124 (PTPN11) | S580 | Uniprot |
| Q06124 (PTPN11) | S595 | Uniprot |
| Q06187 (BTK) | S180 | Uniprot |
| Q07869 (PPARA) | S179 | Uniprot |
| Q07869 (PPARA) | S230 | Uniprot |
| Q12906 (ILF3) | S647 | Uniprot |
| Q13224 (GRIN2B) | S1303 | Uniprot |
| Q13224 (GRIN2B) | S1323 | Uniprot |
| Q14847 (LASP1) | S146 | Uniprot |
| Q16236 (NFE2L2) | S40 | Uniprot |
| Q16625-1 (OCLN) | S340 | Uniprot |
| Q16625 (OCLN) | S490 | Uniprot |
| Q5JVS0-1 (HABP4) | T354 | Uniprot |
| Q5JVS0 (HABP4) | T375 | Uniprot |
| Q5T7P8 (SYT6) | T283 | Uniprot |
| Q5T7P8 (SYT6) | T417 | Uniprot |
| Q5T7P8 (SYT6) | T418 | Uniprot |
| Q8NER1 (TRPV1) | T705 | Uniprot |
| Q92686 (NRGN) | S36 | Uniprot |
| Q96D31 (ORAI1) | S27 | Uniprot |
| Q96D31 (ORAI1) | S30 | Uniprot |
| Q96PH1-4 (NOX5) | T494 | Uniprot |
| Q96PH1-4 (NOX5) | S498 | Uniprot |
| Q99418-2 (CYTH2) | S391 | Uniprot |
| Q99418-1 (CYTH2) | S392 | Uniprot |
| Q9BXL7 (CARD11) | S559 | Uniprot |
| Q9BXL7 (CARD11) | S644 | Uniprot |
| Q9BXL7 (CARD11) | S652 | Uniprot |
| Q9H1D0 (TRPV6) | S184 | Uniprot |
| Q9H1D0 (TRPV6) | T728 | Uniprot |
| Q9P2D0 (IBTK) | S1200 | Uniprot |
| Q9P2D0 (IBTK) | S1203 | Uniprot |
| Q9UBS0 (RPS6KB2) | S473 | Uniprot |
| Q9UBU3 (GHRL) | S41 | Uniprot |
| Q9UQ80-1 (PA2G4) | S363 | Uniprot |
| Q9UQ80-1 (PA2G4) | T366 | Uniprot |
| Q9Y4H2 (IRS2) | S306 | Uniprot |
| Q9Y4H2 (IRS2) | S679 | Uniprot |
研究背景
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.
Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade (By similarity).
Cytoplasm. Nucleus. Membrane>Peripheral membrane protein.
Interacts with PDK1 (By similarity). Interacts in vitro with PRKCBP1. Interacts with PHLPP1 and PHLPP2; both proteins mediate its dephosphorylation. Interacts with KDM1A/LSD1, PKN1 and ANDR.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Substance dependence > Amphetamine addiction.
· Human Diseases > Substance dependence > Morphine addiction.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Glioma. (View pathway)
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Environmental adaptation > Circadian entrainment.
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)
· Organismal Systems > Nervous system > Glutamatergic synapse.
· Organismal Systems > Nervous system > Cholinergic synapse.
· Organismal Systems > Nervous system > Serotonergic synapse.
· Organismal Systems > Nervous system > GABAergic synapse.
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Nervous system > Long-term depression.
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)
· Organismal Systems > Endocrine system > Melanogenesis.
· Organismal Systems > Endocrine system > Thyroid hormone synthesis.
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.
· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.
· Organismal Systems > Digestive system > Salivary secretion.
· Organismal Systems > Digestive system > Gastric acid secretion.
· Organismal Systems > Digestive system > Pancreatic secretion.
· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.