PLCG2 Antibody - #AF7738
(1)
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製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7738, RRID:AB_2844102.
折りたたみ／展開
1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2; 1-phosphatidylinositol-4; 5-bisphosphate phosphodiesterase gamma-2; EC 3.1.4.11; Phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-2; Phospholipase C gamma 2; Phospholipase C, gamma 2 (phosphatidylinositol specific); Phospholipase C-gamma-2; Phospholipase C-IV; PLC 2; PLC gamma 2; PLC IV; PLC-gamma-2; PLC-IV; Plcg2; PLCG2_HUMAN;
免疫原
- P16885 PLCG2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTTVNVDSLAEYEKSQIKRALELGTVMTVFSFRKSTPERRTVQVIMETRQVAWSKTADKIEGFLDIMEIKEIRPGKNSKDFERAKAVRQKEDCCFTILYGTQFVLSTLSLAADSKEDAVNWLSGLKILHQEAMNASTPTIIESWLRKQIYSVDQTRRNSISLRELKTILPLINFKVSSAKFLKDKFVEIGAHKDELSFEQFHLFYKKLMFEQQKSILDEFKKDSSVFILGNTDRPDASAVYLHDFQRFLIHEQQEHWAQDLNKVRERMTKFIDDTMRETAEPFLFVDEFLTYLFSRENSIWDEKYDAVDMQDMNNPLSHYWISSSHNTYLTGDQLRSESSPEAYIRCLRMGCRCIELDCWDGPDGKPVIYHGWTRTTKIKFDDVVQAIKDHAFVTSSFPVILSIEEHCSVEQQRHMAKAFKEVFGDLLLTKPTEASADQLPSPSQLREKIIIKHKKLGPRGDVDVNMEDKKDEHKQQGELYMWDSIDQKWTRHYCAIADAKLSFSDDIEQTMEEEVPQDIPPTELHFGEKWFHKKVEKRTSAEKLLQEYCMETGGKDGTFLVRESETFPNDYTLSFWRSGRVQHCRIRSTMEGGTLKYYLTDNLTFSSIYALIQHYRETHLRCAEFELRLTDPVPNPNPHESKPWYYDSLSRGEAEDMLMRIPRDGAFLIRKREGSDSYAITFRARGKVKHCRINRDGRHFVLGTSAYFESLVELVSYYEKHSLYRKMRLRYPVTPELLERYNMERDINSLYDVSRMYVDPSEINPSMPQRTVKALYDYKAKRSDELSFCRGALIHNVSKEPGGWWKGDYGTRIQQYFPSNYVEDISTADFEELEKQIIEDNPLGSLCRGILDLNTYNVVKAPQGKNQKSFVFILEPKQQGDPPVEFATDRVEELFEWFQSIREITWKIDTKENNMKYWEKNQSIAIELSDLVVYCKPTSKTKDNLENPDFREIRSFVETKADSIIRQKPVDLLKYNQKGLTRVYPKGQRVDSSNYDPFRLWLCGSQMVALNFQTADKYMQMNHALFSLNGRTGYVLQPESMRTEKYDPMPPESQRKILMTLTVKVLGARHLPKLGRSIACPFVEVEICGAEYDNNKFKTTVVNDNGLSPIWAPTQEKVTFEIYDPNLAFLRFVVYEEDMFSDPNFLAHATYPIKAVKSGFRSVPLKNGYSEDIELASLLVFCEMRPVLESEEELYSSCRQLRRRQEELNNQLFLYDTHQNLRNANRDALVKEFSVNENQLQLYQEKCNKRLREKRVSNSKFYS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P16885 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| S160 | Phosphorylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| Y242 | Phosphorylation | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| Y371 | Phosphorylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| Y482 | Phosphorylation | Uniprot | |
| Y495 | Phosphorylation | Uniprot | |
| K557 | Ubiquitination | Uniprot | |
| Y647 | Phosphorylation | Uniprot | |
| S677 | Phosphorylation | Uniprot | |
| S679 | Phosphorylation | Uniprot | |
| Y680 | Phosphorylation | Uniprot | |
| T683 | Phosphorylation | Uniprot | |
| Y720 | Phosphorylation | Uniprot | |
| Y733 | Phosphorylation | Uniprot | |
| Y743 | Phosphorylation | P07948 (LYN) , P06241 (FYN) , P06239 (LCK) | Uniprot |
| Y753 | Phosphorylation | P08631 (HCK) , P12931 (SRC) , P42680 (TEC) , P06241 (FYN) , P06239 (LCK) , Q06187 (BTK) , P07948 (LYN) | Uniprot |
| S756 | Phosphorylation | Uniprot | |
| Y759 | Phosphorylation | Q06187 (BTK) , P43405 (SYK) , P06241 (FYN) , P12931 (SRC) , P42680 (TEC) , P08631 (HCK) , P06239 (LCK) , P07948 (LYN) | Uniprot |
| S763 | Phosphorylation | Uniprot | |
| K775 | Ubiquitination | Uniprot | |
| Y778 | Phosphorylation | Uniprot | |
| Y780 | Phosphorylation | Uniprot | |
| S789 | Phosphorylation | Uniprot | |
| Y811 | Phosphorylation | Uniprot | |
| Y818 | Phosphorylation | Uniprot | |
| T857 | Phosphorylation | Uniprot | |
| Y858 | Phosphorylation | Uniprot | |
| K862 | Ubiquitination | Uniprot | |
| Y936 | Phosphorylation | Uniprot | |
| S941 | Phosphorylation | Uniprot | |
| S957 | Phosphorylation | Uniprot | |
| K962 | Ubiquitination | Uniprot | |
| S965 | Phosphorylation | Uniprot | |
| K980 | Ubiquitination | Uniprot | |
| S994 | Phosphorylation | Uniprot | |
| S1007 | Phosphorylation | Uniprot | |
| T1016 | Phosphorylation | Uniprot | |
| Y1020 | Phosphorylation | Uniprot | |
| S1029 | Phosphorylation | Uniprot | |
| T1034 | Phosphorylation | Uniprot | |
| Y1036 | Phosphorylation | Uniprot | |
| T1045 | Phosphorylation | Uniprot | |
| K1047 | Ubiquitination | Uniprot | |
| Y1048 | Phosphorylation | Uniprot | |
| T1062 | Phosphorylation | Uniprot | |
| T1064 | Phosphorylation | Uniprot | |
| Y1137 | Phosphorylation | Uniprot | |
| S1164 | Phosphorylation | Uniprot | |
| Y1197 | Phosphorylation | Q06187 (BTK) , P06239 (LCK) | Uniprot |
| Y1217 | Phosphorylation | Q06187 (BTK) , P06239 (LCK) | Uniprot |
| T1219 | Phosphorylation | Uniprot | |
| K1233 | Ubiquitination | Uniprot | |
| S1236 | Phosphorylation | Uniprot | |
| Y1245 | Phosphorylation | Uniprot | |
| K1248 | Ubiquitination | Uniprot | |
| S1259 | Phosphorylation | Uniprot | |
| S1261 | Phosphorylation | Uniprot | |
| K1262 | Ubiquitination | Uniprot | |
| Y1264 | Phosphorylation | Uniprot | |
| S1265 | Phosphorylation | Uniprot |
研究背景
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Phosphorylated on tyrosine residues by CSF1R (By similarity). Phosphorylated on tyrosine residues by BTK and SYK; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Phosphorylation leads to increased phospholipase activity.
Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated).
研究領域
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Glioma. (View pathway)
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
参考文献
Application: WB Species: Mouse Sample: RBL-2H3 cells
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