UBF1 Antibody - #AF7743
(1)
| 製品: | UBF1 Antibody |
| カタログ: | AF7743 |
| タンパク質の説明: | Rabbit polyclonal antibody to UBF1 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat, Monkey |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 90KD; 89kD(Calculated). |
| ユニプロット: | P17480 |
| RRID: | AB_2844107 |
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
UBF1 Antibody detects endogenous levels of total UBF1.
RRID:
AB_2844107
引用形式: Affinity Biosciences Cat# AF7743, RRID:AB_2844107.
引用形式: Affinity Biosciences Cat# AF7743, RRID:AB_2844107.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
90 kDa nucleolus organizer region autoantigen; Autoantigen NOR-90; NOR 90; Nucleolar transcription factor 1; UBF 1; UBF; UBF-1; UBF1_HUMAN; Ubtf; Upstream binding factor 1; upstream binding transcription factor, RNA polymerase I; Upstream-binding factor 1;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
- P17480 UBF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKLKWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPLTPYFRFFMEKRAKYAKLHPEMSNLDLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPDLIQNAKKSDIPEKPKTPQQLWYTHEKKVYLKVRPDATTKEVKDSLGKQWSQLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKSTLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGEREERGKLPESPKRAEEIWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTTLQSKSESEEDDEEDEDDEDEDEEEEDDENGDSSEDGGDSSESSSEDESEDGDENEEDDEDEDDDEDDDEDEDNESEGSSSSSSSSGDSSDSDSN
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P17480 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| M1 | Acetylation | Uniprot | |
| T9 | Phosphorylation | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K61 | Acetylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K114 | Acetylation | Uniprot | |
| K114 | Sumoylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| T117 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| K126 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| Y150 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K160 | Sumoylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| T201 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| Y207 | Phosphorylation | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K232 | Methylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K254 | Methylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K284 | Acetylation | Uniprot | |
| K284 | Sumoylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| Y308 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| K352 | Acetylation | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K385 | Acetylation | Uniprot | |
| T388 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S392 | Phosphorylation | Uniprot | |
| K394 | Acetylation | Uniprot | |
| K401 | Acetylation | Uniprot | |
| K406 | Acetylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| R429 | Methylation | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| R443 | Methylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| Y456 | Phosphorylation | Uniprot | |
| K464 | Sumoylation | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| S484 | Phosphorylation | P24941 (CDK2) , P11802 (CDK4) | Uniprot |
| S495 | Phosphorylation | Uniprot | |
| Y500 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| K559 | Acetylation | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| K624 | Ubiquitination | Uniprot | |
| S638 | Phosphorylation | Uniprot | |
| R642 | Methylation | Uniprot | |
| K646 | Ubiquitination | Uniprot | |
| Y648 | Phosphorylation | Uniprot | |
| S655 | Phosphorylation | Uniprot | |
| S666 | Phosphorylation | Uniprot |
研究背景
機能:
Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
PTMs:
Phosphorylated and activated by PIK3CA.
細胞の位置付け:
Nucleus>Nucleolus.
サブユニット構造:
Homodimer (By similarity). Interacts with TBP. Interacts with TAF1A. Interacts with RASL11A (By similarity). Binds to IRS1 and PIK3CA (By similarity). Interacts with DHX33. Interacts with PHF6. Interacts with CEBPA (isoform 1 and isoform 4). Interacts with DDX11. Interacts with NOP53.
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