ATP citrate lyase Antibody - #AF7823

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製品: ATP citrate lyase Antibody
カタログ: AF7823
タンパク質の説明: Rabbit polyclonal antibody to ATP citrate lyase
アプリケーション: WB
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 125kDa; 121kD(Calculated).
ユニプロット: P53396
RRID: AB_2844187

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HPLC Report
MSDS
説明書
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(90%)
クローナリティ:
Polyclonal
特異性:
ATP citrate lyase Antibody detects endogenous levels of total ATP citrate lyase.
RRID:
AB_2844187
引用形式: Affinity Biosciences Cat# AF7823, RRID:AB_2844187.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ACL; Acly; ACLY_HUMAN; ATP citrate (pro-S) lyase; ATP citrate lyase; ATP citrate synthase; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; ATPcitrate synthase; ATPCL; Citrate cleavage enzyme; CLATP; OTTHUMP00000164773;

免疫原

免疫原:
Uniprot:

P53396(ACLY_HUMAN) >>Visit HPA database.

遺伝子(ID):
ACLY(47)
タンパク質配列:
MSAKAISEQTGKELLYKFICTTSAIQNRFKYARVTPDTDWARLLQDHPWLLSQNLVVKPDQLIKRRGKLGLVGVNLTLDGVKSWLKPRLGQEATVGKATGFLKNFLIEPFVPHSQAEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNPEDIKKHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKDGVYVLDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRADEVAPAKKAKPAMPQDSVPSPRSLQGKSTTLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMRKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPIVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVANGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
90
Chicken
90
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53396 基板として

Site PTM Type Enzyme
K4 Ubiquitination
K12 Ubiquitination
K17 Ubiquitination
C20 S-Nitrosylation
S23 Phosphorylation
T38 Phosphorylation
K58 Ubiquitination
K64 Acetylation
K64 Ubiquitination
K68 Ubiquitination
K82 Ubiquitination
S83 Phosphorylation
K86 Acetylation
K86 Ubiquitination
T94 Phosphorylation
K97 Ubiquitination
T99 Phosphorylation
Y131 Phosphorylation
K148 Ubiquitination
K151 Acetylation
K151 Ubiquitination
K159 Acetylation
K159 Ubiquitination
K166 Ubiquitination
K167 Ubiquitination
Y213 Phosphorylation
C229 S-Nitrosylation
K230 Ubiquitination
K232 Ubiquitination
Y247 Phosphorylation
Y252 Phosphorylation
K259 Acetylation
K259 Ubiquitination
S260 Phosphorylation
S263 Phosphorylation
K265 Ubiquitination
K272 Ubiquitination
Y317 Phosphorylation
T322 Phosphorylation
S325 Phosphorylation
Y364 Phosphorylation
K369 Acetylation
K369 Ubiquitination
R379 Methylation
Y384 Phosphorylation
R389 Methylation
S442 Phosphorylation
S444 Phosphorylation
T445 Phosphorylation
S446 Phosphorylation
T447 Phosphorylation
S451 Phosphorylation
T453 Phosphorylation
S455 Phosphorylation
S457 Phosphorylation
S459 Phosphorylation
K468 Ubiquitination
K471 Ubiquitination
S478 Phosphorylation
S481 Phosphorylation
S484 Phosphorylation
K488 Ubiquitination
K498 Ubiquitination
Y531 Phosphorylation
K538 Acetylation
K538 Ubiquitination
K540 Acetylation
K540 Ubiquitination
K546 Acetylation
K546 Ubiquitination
K554 Acetylation
K554 Ubiquitination
K562 Ubiquitination
S574 Phosphorylation
S581 Phosphorylation
T582 Phosphorylation
Y588 Phosphorylation
T593 Phosphorylation
T606 Phosphorylation
K616 Ubiquitination
K630 Acetylation
K630 Ubiquitination
K635 Acetylation
K635 Ubiquitination
T639 Phosphorylation
S649 Phosphorylation
K650 Ubiquitination
Y652 Phosphorylation
S656 Phosphorylation
Y659 Phosphorylation
S663 Phosphorylation
S667 Phosphorylation
S675 Phosphorylation
T677 Phosphorylation
Y682 Phosphorylation
R691 Methylation
Y692 Phosphorylation
T707 Phosphorylation
H760 Phosphorylation
K780 Ubiquitination
K836 Ubiquitination
S839 Phosphorylation
Y854 Phosphorylation
K918 Ubiquitination
T930 Phosphorylation
K944 Ubiquitination
K948 Acetylation
K948 Ubiquitination
S952 Phosphorylation
K962 Acetylation
K962 Ubiquitination
K964 Methylation
K965 Methylation
K968 Acetylation
K968 Ubiquitination
K978 Acetylation
K978 Ubiquitination
S979 Phosphorylation
R986 Methylation
K991 Ubiquitination
T1001 Phosphorylation
K1012 Ubiquitination
Y1073 Phosphorylation
K1077 Acetylation
K1077 Ubiquitination
K1080 Ubiquitination
Y1084 Phosphorylation
S1100 Phosphorylation

研究背景

機能:

Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.

PTMs:

Phosphorylated by PKA and GSK3 in a sequential manner; phosphorylation results in activation of its activity. Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455 (By similarity). Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity).

ISGylated.

Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.

Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (Probable).

細胞の位置付け:

Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homotetramer.

タンパク質ファミリー:

In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.

In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.

研究領域

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Global and overview maps > Metabolic pathways.

参考文献

1). ATP citrate lyase inhibitor triggers endoplasmic reticulum stress to induce hepatocellular carcinoma cell apoptosis via p‐eIF2α/ATF4/CHOP axis. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021 (PubMed: 33393219) [IF=5.3]

Application: WB    Species: Human    Sample: liver cancer tissue

FIGURE 1 ACLY is highly expressed in HCC. RNA sequencing and microarray gene profiling data from (A) TCGA and (B) GEO was utilized to investigate ACLY expression in HCC samples (n = 370) and non-tumour liver tissue samples (n = 50). C, We also took HCC samples (n = 50) and normal liver samples (n = 50) from TCGA data to assess ACLY expression. D, Western blot was performed to determine the protein expression of ACLY in newly operated HCC (n = 4) and adjacent normal tissue specimens (n = 4) from patients. E, The mRNA level of ACLY was detected in HCC tissues (n = 15) compared with that in the adjacent normal tissues (n = 15) by real-time PCR. (*P < .05, **P < .01, compared with NT group) (F) Western blot and (G) Real-time PCR were adopted to detect the protein and mRNA expressions of ACLY in human normal liver cell line (LO2) and human HCC cell lines (Hep3B, HepG2, HCC-LM3, Huh7), respectively. (**P < .01, compared with LO2 group). All experiments were repeated 3 times

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