SF1 Antibody - #AF7895
(2)
(1)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7895, RRID:AB_2844259.
折りたたみ／展開
BBP; D11S636; Mammalian branch point binding protein mBBP; OTTHUMP00000069749; OTTHUMP00000069750; OTTHUMP00000069752; Splicing factor 1; Transcription factor ZFM1; ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162; ZNF162; SF1;
免疫原
Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
- Q15637 SF01_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERHNLITEMVALNPDFKPPADYKPPATRVSDKVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKEGKVGRKDGQMLPGEDEPLHALVTANTMENVKKAVEQIRNILKQGIETPEDQNDLRKMQLRELARLNGTLREDDNRILRPWQSSETRSITNTTVCTKCGGAGHIASDCKFQRPGDPQSAQDKARMDKEYLSLMAELGEAPVPASVGSTSGPATTPLASAPRPAAPANNPPPPSLMSTTQSRPPWMNSGPSESRPYHGMHGGGPGGPGGGPHSFPHPLPSLTGGHGGHPMQHNPNGPPPPWMQPPPPPMNQGPHPPGHHGPPPMDQYLGSTPVGSGVYRLHQGKGMMPPPPMGMMPPPPPPPSGQPPPPPSGPLPPWQQQQQQPPPPPPPSSSMASSTPLPWQQNTTTTTTSAGTGSIPPWQQQQAAAAASPGAPQMQGNPTMVPLPPGVQPPLPPGAPPPPPPPPPGSAGMMYAPPPPPPPPMDPSNFVTMMGMGVAGMPPFGMPPAPPPPPPQN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15637 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| T67 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Q8TAS1 (UHMK1) | Uniprot |
| S82 | Phosphorylation | Q8TAS1 (UHMK1) | Uniprot |
| Y87 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K217 | Acetylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| T232 | Phosphorylation | Uniprot | |
| T253 | Phosphorylation | Uniprot | |
| R263 | Methylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| T277 | Phosphorylation | Uniprot | |
| C279 | S-Nitrosylation | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| K306 | Acetylation | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| Y313 | Phosphorylation | Uniprot | |
| T338 | Phosphorylation | Uniprot | |
| R345 | Methylation | Uniprot | |
| R365 | Methylation | Uniprot |
研究背景
Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463.
Nucleus.
Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
Binds U2AF2. Interacts with U1 snRNA. Binds EWSR1, FUS and TAF15. Interacts with RBM17.
Belongs to the BBP/SF1 family.
参考文献
Application: WB Species: Human Sample: ovarian tissue
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