PI3 Kinase Class III Antibody - #AF7921
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| 製品: | PI3 Kinase Class III Antibody |
| カタログ: | AF7921 |
| タンパク質の説明: | Rabbit polyclonal antibody to PI3 Kinase Class III |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus |
| 分子量: | 100kDa; 102kD(Calculated). |
| ユニプロット: | Q8NEB9 |
| RRID: | AB_2844284 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7921, RRID:AB_2844284.
折りたたみ／展開
hVps34; MGC61518; Phosphatidylinositol 3 kinase catalytic subunit type 3; Phosphatidylinositol 3 kinase class 3; Phosphatidylinositol 3 kinase p100 subunit; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide 3 kinase class 3; Phosphoinositide-3-kinase class 3; PI3 kinase type 3; PI3-kinase type 3; PI3K type 3; Pik3c3; PK3C3_HUMAN; PtdIns 3 kinase type 3; PtdIns-3-kinase type 3; Vps 34; Vps34;
免疫原
- Q8NEB9 PK3C3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGEAEKFHYIYSCDLDINVQLKIGSLEGKREQKSYKAVLEDPMLKFSGLYQETCSDLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNAQVALTIWDVYGPGKAVPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEADGSEPTKTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQMSMENLVESKHHKLARSLRSGPSDHDLKPNAATRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFLKCVNWDLPQEAKQALELLGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALKYENFDDIKNGLEPTKKDSQSSVSENVSNSGINSAEIDSSQIITSPLPSVSSPPPASKTKEVPDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQDTQQRDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKAVQRESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPETATLFKSALMPAQLFFKTEDGGKYPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQNFFRKYAPSENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8NEB9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S25 | Phosphorylation | Uniprot | |
| K29 | Acetylation | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| K36 | Methylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| T159 | Phosphorylation | P06493 (CDK1) , Q00535 (CDK5) | Uniprot |
| T163 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| K287 | Methylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| Y310 | Phosphorylation | Uniprot | |
| Y321 | Phosphorylation | Uniprot | |
| Y322 | Phosphorylation | Uniprot | |
| T324 | Phosphorylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| Y379 | Phosphorylation | Uniprot | |
| Y395 | Phosphorylation | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| K463 | Ubiquitination | Uniprot | |
| T488 | Phosphorylation | Uniprot | |
| K582 | Ubiquitination | Uniprot | |
| K600 | Ubiquitination | Uniprot | |
| K613 | Methylation | Uniprot | |
| T625 | Phosphorylation | Uniprot | |
| K630 | Ubiquitination | Uniprot | |
| Y631 | Phosphorylation | Uniprot | |
| K666 | Ubiquitination | Uniprot | |
| T668 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| T677 | Phosphorylation | Uniprot | |
| K706 | Ubiquitination | Uniprot | |
| K771 | Acetylation | Uniprot | |
| K781 | Acetylation | Uniprot | |
| K781 | Ubiquitination | Uniprot | |
| Y803 | Phosphorylation | Uniprot | |
| K840 | Sumoylation | Uniprot | |
| K845 | Sumoylation | Uniprot | |
| Y884 | Phosphorylation | Uniprot |
研究背景
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity).
Midbody. Late endosome. Cytoplasmic vesicle>Autophagosome.
Note: As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (Probable). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity).
Ubiquitously expressed, with a highest expression in skeletal muscle.
Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxilliary subunits to form alternative complex forms. Alternative complex forms containing a forth regulatory subunit in a mutually exclusive manner are: the PI3K complex I (PI3KC3-C1) containing ATG14, and the PI3K complex II (PI3KC3-C2) containing UVRAG. PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex. Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits such as RUBCN, SH3GLB1/Bif-1 and AMBRA1. PI3KC3-C1 probably associates with PIK3CB (By similarity). Interacts with RAB7A in the presence of PIK3R4. Interacts with AMBRA1 (By similarity). Interacts with BECN1P1/BECN2. Interacts with SLAMF1. May be a component of a complex composed of RAB5A (in GDP-bound form), DYN2 and PIK3C3 (By similarity). Interacts with NCKAP1L.
Belongs to the PI3/PI4-kinase family.
研究領域
· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
参考文献
Application: WB Species: rat Sample: spleen
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