PBK/TOPK Antibody - #AF7932
(1)
(1)
| 製品: | PBK/TOPK Antibody |
| カタログ: | AF7932 |
| タンパク質の説明: | Rabbit polyclonal antibody to PBK/TOPK |
| アプリケーション: | WB |
| 反応性: | Human |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 36kDa; 36kD(Calculated). |
| ユニプロット: | Q96KB5 |
| RRID: | AB_2844295 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7932, RRID:AB_2844295.
折りたたみ／展開
Cancer/testis antigen 84; CT84; Epididymis luminal protein 164; FLJ14385; HEL164; Lymphokine activated killer T cell originated protein kinase; Lymphokine-activated killer T-cell-originated protein kinase; MAPKK like protein kinase; MAPKK-like protein kinase; Nori 3; Nori-3; Nori3; PBK; PDZ binding kinase; PDZ-binding kinase; Serine/threonine protein kinase; Spermatogenesis related protein kinase; Spermatogenesis-related protein kinase; SPK; T LAK cell originated protein kinase; T-LAK cell-originated protein kinase; TOPK; TOPK_HUMAN;
免疫原
Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
- Q96KB5 TOPK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEGISNFKTPSKLSEKKKSVLCSTPTINIPASPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPICNDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGSLCLAMEYGGEKSLNDLIEERYKASQDPFPAAIILKVALNMARGLKYLHQEKKLLHGDIKSSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTEPWKPKEAVEENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPPINMEELDESYQKVIELFSVCTNEDPKDRPSAAHIVEALETDV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96KB5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| K8 | Sumoylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| T9 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S11 | Phosphorylation | Uniprot | |
| K12 | Acetylation | Uniprot | |
| K12 | Sumoylation | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K16 | Acetylation | Uniprot | |
| K17 | Acetylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| T24 | Phosphorylation | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| Y47 | Phosphorylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| S59 | Phosphorylation | Uniprot | |
| K64 | Acetylation | Uniprot | |
| K64 | Sumoylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K65 | Sumoylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| Y74 | Phosphorylation | P12931 (SRC) | Uniprot |
| S76 | Phosphorylation | Uniprot | |
| Y78 | Phosphorylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K87 | Acetylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| Y100 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K155 | Sumoylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K162 | Sumoylation | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K169 | Sumoylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| K176 | Sumoylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| T198 | Phosphorylation | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| Y272 | Phosphorylation | P12931 (SRC) | Uniprot |
| S310 | Phosphorylation | Uniprot |
PTMs - Q96KB5 酵素として
研究背景
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Phosphorylated; in a cell-cycle dependent manner at mitosis.
Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
Interacts with DLG1 and TP53.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
参考文献
Application: WB Species: Human Sample: breast tissue
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.