製品: Myt1 Antibody
カタログ: AF7935
タンパク質の説明: Rabbit polyclonal antibody to Myt1
アプリケーション: WB IF/ICC
反応性: Human
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 70kDa; 55kD(Calculated).
ユニプロット: Q99640
RRID: AB_2844298

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Myt1 Antibody detects endogenous levels of total Myt1.
RRID:
AB_2844298
引用形式: Affinity Biosciences Cat# AF7935, RRID:AB_2844298.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

cdc2 inhibitory kinase; DKFZp547K1610; FLJ20093; Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; MYT1; Myt1 kinase; PKMYT 1; Pkmyt1; PMYT1_HUMAN; PPP1R126; Protein kinase membrane associated tyrosine/threonine 1; Protein kinase Myt1; Protein phosphatase 1 regulatory subunit 126;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MLERPPALAMPMPTEGTPPPLSGTPIPVPAYFRHAEPGFSLKRPRGLSRSLPPPPPAKGSIPISRLFPPRTPGWHQLQPRRVSFRGEASETLQSPGYDPSRPESFFQQSFQRLSRLGHGSYGEVFKVRSKEDGRLYAVKRSMSPFRGPKDRARKLAEVGSHEKVGQHPCCVRLEQAWEEGGILYLQTELCGPSLQQHCEAWGASLPEAQVWGYLRDTLLALAHLHSQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGTAGAGEVQEGDPRYMAPELLQGSYGTAADVFSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRSVLVMMLEPDPKLRATAEALLALPVLRQPRAWGVLWCMAAEALSRGWALWQALLALLCWLWHGLAHPASWLQPLGPPATPPGSPPCSLLLDSSLSSNWDDDSLGPSLSPEAVLARTVGSTSTPRSRCTPRDALDLSDINSEPPRGSFPSFEPRNLLSLFEDTLDPT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
92
Zebrafish
73
Xenopus
67
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99640 基板として

Site PTM Type Enzyme
Phosphorylation
M1 Acetylation
T17 Phosphorylation
T24 Phosphorylation
S40 Phosphorylation
K42 Methylation
K42 Ubiquitination
S48 Phosphorylation
S50 Phosphorylation
K58 Ubiquitination
S60 Phosphorylation
S64 Phosphorylation
T71 Phosphorylation
S83 Phosphorylation P31749 (AKT1) , P45983 (MAPK8)
S89 Phosphorylation
S94 Phosphorylation
Y97 Phosphorylation
S109 Phosphorylation
S120 Phosphorylation
K126 Methylation
K126 Ubiquitination
K139 Ubiquitination
S141 Phosphorylation
S143 Phosphorylation
K149 Ubiquitination
K154 Ubiquitination
S160 Phosphorylation
K163 Ubiquitination
K345 Ubiquitination
T412 Phosphorylation
S416 Phosphorylation
S426 Phosphorylation P53350 (PLK1)
S441 Phosphorylation
T453 Phosphorylation
S454 Phosphorylation
T455 Phosphorylation
S458 Phosphorylation
T461 Phosphorylation
S469 Phosphorylation
S473 Phosphorylation
S479 Phosphorylation
S482 Phosphorylation
T495 Phosphorylation P53350 (PLK1)

PTMs - Q99640 酵素として

Substrate Site Source
P06493 (CDK1) T14 Uniprot
P06493 (CDK1) Y15 Uniprot

研究背景

機能:

Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.

PTMs:

Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.

細胞の位置付け:

Endoplasmic reticulum membrane>Peripheral membrane protein. Golgi apparatus membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1.

タンパク質ファミリー:

The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

研究領域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

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