LATS2 Antibody - #AF7940

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製品: LATS2 Antibody
カタログ: AF7940
タンパク質の説明: Rabbit polyclonal antibody to LATS2
アプリケーション: WB
反応性: Human, Mouse
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 150kDa; 120kD(Calculated).
ユニプロット: Q9NRM7
RRID: AB_2844303

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
LATS2 Antibody detects endogenous levels of total LATS2.
RRID:
AB_2844303
引用形式: Affinity Biosciences Cat# AF7940, RRID:AB_2844303.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FLJ13161; Kinase phosphorylated during mitosis protein; KPM; Large tumor suppressor homolog 2; Large tumor suppressor homolog 2 Drosophila; Large tumor suppressor kinase 2; LATS large tumor suppressor Drosophila homolog 2; LATS large tumor suppressor homolog 2; Lats2; LATS2_HUMAN; Serine/threonine kinase kpm; Serine/threonine protein kinase kpm; Serine/threonine-protein kinase kpm; Serine/threonine-protein kinase LATS2; Warts like kinase; Warts-like kinase;

免疫原

免疫原:
Uniprot:

Q9NRM7(LATS2_HUMAN) >>Visit HPA database.

遺伝子(ID):
LATS2(26524)
発現特異性:
Q9NRM7 LATS2_HUMAN:

Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.

タンパク質配列:
MRPKTFPATTYSGNSRQRLQEIREGLKQPSKSSVQGLPAGPNSDTSLDAKVLGSKDATRQQQQMRATPKFGPYQKALREIRYSLLPFANESGTSAAAEVNRQMLQELVNAGCDQEMAGRALKQTGSRSIEAALEYISKMGYLDPRNEQIVRVIKQTSPGKGLMPTPVTRRPSFEGTGDSFASYHQLSGTPYEGPSFGADGPTALEEMPRPYVDYLFPGVGPHGPGHQHQHPPKGYGASVEAAGAHFPLQGAHYGRPHLLVPGEPLGYGVQRSPSFQSKTPPETGGYASLPTKGQGGPPGAGLAFPPPAAGLYVPHPHHKQAGPAAHQLHVLGSRSQVFASDSPPQSLLTPSRNSLNVDLYELGSTSVQQWPAATLARRDSLQKPGLEAPPRAHVAFRPDCPVPSRTNSFNSHQPRPGPPGKAEPSLPAPNTVTAVTAAHILHPVKSVRVLRPEPQTAVGPSHPAWVPAPAPAPAPAPAPAAEGLDAKEEHALALGGAGAFPLDVEYGGPDRRCPPPPYPKHLLLRSKSEQYDLDSLCAGMEQSLRAGPNEPEGGDKSRKSAKGDKGGKDKKQIQTSPVPVRKNSRDEEKRESRIKSYSPYAFKFFMEQHVENVIKTYQQKVNRRLQLEQEMAKAGLCEAEQEQMRKILYQKESNYNRLKRAKMDKSMFVKIKTLGIGAFGEVCLACKVDTHALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVKLYYSFQDKDSLYFVMDYIPGGDMMSLLIRMEVFPEHLARFYIAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGSHVRQDSMEPSDLWDDVSNCRCGDRLKTLEQRARKQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQLKVINWENTLHIPAQVKLSPEARDLITKLCCSADHRLGRNGADDLKAHPFFSAIDFSSDIRKQPAPYVPTISHPMDTSNFDPVDEESPWNDASEGSTKAWDTLTSPNNKHPEHAFYEFTFRRFFDDNGYPFRCPKPSGAEASQAESSDLESSDLVDQTEGCQPVYV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NRM7 基板として

Site PTM Type Enzyme
T5 Phosphorylation
T9 Phosphorylation
T10 Phosphorylation
Y11 Phosphorylation
S12 Phosphorylation
S43 Phosphorylation
K50 Ubiquitination
K55 Acetylation
K55 Ubiquitination
Y82 Phosphorylation
S83 Phosphorylation O14965 (AURKA)
Y135 Phosphorylation
K154 Ubiquitination
S172 Phosphorylation O14757 (CHEK1) , O96017 (CHEK2)
S274 Phosphorylation
K278 Ubiquitination
T279 Phosphorylation
Y286 Phosphorylation
S288 Phosphorylation
S342 Phosphorylation
S380 Phosphorylation O14965 (AURKA) , O14757 (CHEK1) , O96017 (CHEK2)
S408 Phosphorylation O96017 (CHEK2) , O14757 (CHEK1)
S446 Phosphorylation O96017 (CHEK2) , O14757 (CHEK1)
S528 Phosphorylation
Y531 Phosphorylation
S535 Phosphorylation
S543 Phosphorylation
K565 Acetylation
K570 Acetylation
K571 Acetylation
T575 Phosphorylation
S576 Phosphorylation
S598 Phosphorylation
K620 Ubiquitination
K651 Ubiquitination
T690 Phosphorylation
K697 Ubiquitination
K793 Ubiquitination
K827 Ubiquitination
S829 Phosphorylation
S835 Phosphorylation Q9NRM7 (LATS2) , O14757 (CHEK1)
S872 Phosphorylation Q13043 (STK4) , Q13188 (STK3)
K939 Ubiquitination
K950 Ubiquitination
K968 Ubiquitination
K984 Ubiquitination
T1024 Phosphorylation
T1026 Phosphorylation
S1027 Phosphorylation
Y1038 Phosphorylation
T1041 Phosphorylation Q13188 (STK3) , Q13043 (STK4)
Y1051 Phosphorylation

PTMs - Q9NRM7 酵素として

Substrate Site Source
O95863 (SNAI1) T203 Uniprot
P00519-2 (ABL1) T197 Uniprot
P38936 (CDKN1A) S146 Uniprot
P46937 (YAP1) S127 Uniprot
P46937 (YAP1) S397 Uniprot
P61981 (YWHAG) S59 Uniprot
Q02241 (KIF23) S716 Uniprot
Q02241 (KIF23) S814 Uniprot
Q4VCS5 (AMOT) S175 Uniprot
Q8NHZ8 (CDC26) T7 Uniprot
Q9GZV5 (WWTR1) S89 Uniprot
Q9NQS7 (INCENP) S894 Uniprot
Q9NRM7 (LATS2) S835 Uniprot
Q9Y2J4 (AMOTL2) S159 Uniprot

研究背景

機能:

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.

PTMs:

Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3/MST2.

細胞の位置付け:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole. Nucleus.
Note: Colocalizes with AURKA at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Translocates to the nucleus upon mitotic stress by nocodazole treatment.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.

サブユニット構造:

Interacts with and is phosphorylated by AURKA. Binds to AR. Interacts with AJUBA during mitosis and this complex regulates organization of the spindle apparatus through recruitment of gamma-tubulin to the centrosome. Interacts (via PPxY motif) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with SNAI1.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

研究領域

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

参考文献

1). LATS1 is a central signal transmitter for achieving full type-I interferon activity. Science Advances, 2022 (PubMed: 35394840) [IF=13.6]
2). Sophoridine inhibits lung cancer cell growth and enhances cisplatin sensitivity through activation of the p53 and Hippo signaling pathways. Gene, 2020 (PubMed: 32165304) [IF=3.5]

Application: WB    Species: human    Sample: NCI-H460、A549 cells and p53-deficient NCI-H1299 cells

Fig. 4.| Sophoridine activates the Hippo pathway and induces mRNA expression of Hippo downstream genes. a Sophoridine induced protein expression of core components of the Hippo pathway(MST1/2 and LATS1/2) and phosphorylated YAP and suppressed YAP protein and the Hippo target gene CTGF in NCI-H460 and A549 cells but not in p53-deficient NCI-H1299 cells. Cells were treated with DMSO or sophoridine (5 or 10 μg/mL) for 48 h.

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