Phospho-STAT3 (Ser727) Antibody | Affinity Biosciences

製品:	Phospho-STAT3 (Ser727) Antibody
カタログ:	AF3294
タンパク質の説明:	Rabbit polyclonal antibody to Phospho-STAT3 (Ser727)
アプリケーション:	WB IHC IF/ICC IP
Cited expt.:	WB
反応性:	Human, Mouse, Rat
予測:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken
分子量:	86kDa; 88kD(Calculated).
ユニプロット:	P40763
RRID:	AB_2834713

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サイズ	価格	在庫状況
100ul	$280	在庫あり
200ul	$350	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, IP 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat

予測:

Pig(91%), Bovine(91%), Horse(91%), Sheep(82%), Rabbit(91%), Chicken(91%)

クローナリティ:

Polyclonal

特異性:

Phospho-STAT3 (Ser727) Antibody detects endogenous levels of STAT3 only when phosphorylated at Serine 727.

RRID:

AB_2834713
引用形式: Affinity Biosciences Cat# AF3294, RRID:AB_2834713.

コンジュゲート:

Unconjugated.

精製:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

1110034C02Rik; Acute Phase Response Factor; Acute-phase response factor; ADMIO; APRF; AW109958; DNA binding protein APRF; FLJ20882; HIES; MGC16063; Signal transducer and activator of transcription 3 (acute phase response factor); Signal transducer and activator of transcription 3; STAT 3; Stat3; STAT3_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human STAT3 around the phosphorylation site of Ser727.

Uniprot:

P40763(STAT3_HUMAN) >>Visit HPA database.

遺伝子(ID):

STAT3(6774)

発現特異性:

P40763 STAT3_HUMAN:

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

タンパク質の説明:

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.

タンパク質配列:

P40763 STAT3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Chicken

Rabbit

Sheep

Zebrafish

Dog

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

P40763_STAT3_HUMAN

機能:

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1. Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity).

PTMs:

Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.

Acetylated on lysine residues by CREBBP. Deacetylation by LOXL3 leads to disrupt STAT3 dimerization and inhibit STAT3 transcription activity. Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated.

Some lysine residues are oxidized to allysine by LOXL3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity. Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated.

(Microbial infection) Phosphorylated on Tyr-705 in the presence of S.typhimurium SarA.

S-palmitoylated by ZDHHC19 in SH2 putative lipid-binding pockets, leading to homodimerization. Nuclear STAT3 is highly palmitoylated (about 75%) compared with cytoplasmic STAT3 (about 20%).

S-stearoylated, probably by ZDHHC19.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.

組織特異性:

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

タンパク質ファミリー:

Belongs to the transcription factor STAT family.

研究領域

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

参考文献

1). Cancer-associated Fibroblast-like Cells Promote Osteosarcoma Metastasis by Upregulation of Phosphoserine Aminotransferase 1 and Activation of the mTOR/S6K Pathway. International journal of biological sciences, 2025 (PubMed: 40612679) [IF=8.2]

Application: WB Species: human Sample: WI-38 cells

Figure 1. Obtaining of CAF-like cells. (A) The morphological images of NFs and CAF-like cells, scale bars, 50 µm. CAF-like cells were treated with HOS-CM, while NFs were cultured in MEM. (B) Relative mRNA expression of CAF biomarkers in WI-38 and HELF cells by q-PCR, the indicated results represent the mean ± SD (n=4), * P < 0.05, ** P < 0.01, *** P < 0.001. WI-38 and HELF cells were treated with HOS-CM and 143B-CM, while the control group was the MEM group. (C) The protein level of CAF biomarkers in WI-38 and HELF cells by western blotting analysis. (D) The expression of α-SMA in normal WI-38 and CAF-like cells were assayed by immunofluorescence staining, scale bars, 100 µm. (E) Proliferation ability of NFs and CAF-like cells by CCK-8 assay was displayed as OD value at 450 nm. (F) Migration ability of NFs and CAF-like cells were analyzed by wound-healing assays and expressed as area recovery rate, scale bar, 200 µm. (G) Relative mRNA expression of inflammatory factors in WI-38 by q-PCR, the indicated results represent the mean ± SD (n=3), * P < 0.05, ** P < 0.01, *** P < 0.001. (H) The protein level of IL-6/STAT3 and TGF-β receptor pathways in WI-38 by western blot analysis.

2). Mechanism by which CAP regulates the "cold-heat" balance in osteosarcoma model mice: an integrative study of metabolomics, transcriptomics, and network pharmacology. Journal of translational medicine, 2025 (PubMed: 41146125) [IF=7.4]

Application: WB Species: Mouse Sample:

Fig. 7 Biological validation of key molecular targets.(A-F) Western blot analysis of key proteins. (G) Immunofluorescence analysis. (H) Multiplex IHC showing the expression levels of target molecules in OS model mice. *p

3). A Dichloropropionamide-Substituted Diaminopyrimidine EGFR-TKI Overcomes Osimertinib Resistance in NSCLC via Dual Anchoring at Ser797 and Met793. Journal of medicinal chemistry, 2026 (PubMed: 41428945) [IF=6.8]

4). Hesperidin Inhibits Oxidative Stress and Apoptosis of Granulosa Cells in Polycystic Ovarian Syndrome Through the JAK2/STAT3 and PI3K/AKT Pathways. Phytotherapy research : PTR, 2025 (PubMed: 41014201) [IF=6.1]

5). Mesenchymal Stem Cells Overexpressing ACE2 Favorably Ameliorate LPS-Induced Inflammatory Injury in Mammary Epithelial Cells. Frontiers in Immunology, 2022 (PubMed: 35095873) [IF=5.7]

Application: WB Species: Mice Sample: EpH4-Ev cells

Figure 7 MSC-ACE2 upregulates IL-10/STAT3/SOCS3 signaling pathway expression to suppress LPS-induced inflammation in EpH4-Ev cells. (A) Detection of relative protein expression levels of IL-10, phosphorylation levels of STAT3, STAT3 and SOCS3 by Western blot; (B–D), Statistics of the IL-10, phosphorylation levels of STAT3, STAT3 and SOCS3 Western blot results. Experiments were repeated three times and data were presented as the mean ± SEM (n = 4). * P < 0.05 vs. EpH4-Ev; # P < 0.05 vs. LPS; $ P < 0.05 vs. MSC; & P < 0.05 vs. MSC-GFP.

6). Threonine Deficiency Increases Triglyceride Deposition in Primary Duck Hepatocytes by Reducing STAT3 Phosphorylation. International journal of molecular sciences, 2024 (PubMed: 39125712) [IF=5.6]

7). Gondoic acid alleviates LPS‑induced Kupffer cells inflammation by inhibiting ROS production and PKCθ/ERK/STAT3 signaling pathway. International Immunopharmacology, 2022 (PubMed: 35998508) [IF=4.8]

8). Physical exercise protects neurons from energy deficit and improves cognitive function by upregulating astrocytic Slc2a1 in Alzheimer's disease. Experimental neurology, 2025 (PubMed: 41061790) [IF=4.6]

9). Qing-Luo-Yin Alleviated Experimental Arthritis in Rats by Disrupting Immune Feedback Between Inflammatory T Cells and Monocytes: Key Evidences from Its Effects on Immune Cell Phenotypes. Journal of Inflammation Research, 2023 (PubMed: 35002280) [IF=4.5]

Application: WB Species: Rat Sample:

Figure 6 Effects of QLY-related anti-rheumatic compounds on AIA lymphocytes in vitro. (A) Levels of IFN-γ and IL-17α in the medium released by compounds-treated AIA lymphocytes, determined by ELISA; (B) expression of protein (p)-STAT3 in compounds-treated AIA lymphocytes, assessed by Western blot analysis; (C) quantification of assay B; (D) mRNA expression of IL-4, SIRT1, IFN-γ and IL-17α in compounds-treated AIA lymphocytes, evaluated by RT-qPCR. Statistical significance: *p < 0.05 and **p < 0.01 compared with untreated AIA lymphocytes; #p < 0.05 and ##p < 0.01 compared with AIA lymphocytes receiving combination stimulus (SIN + SCA + MT). Abbreviations: QLY, Qing-Luo-Yin; AIA, adjuvant-induced arthritis; SIN, sinomenine; SCA, sophocarpine; MT, matrine.

10). Umbilical cord‑derived mesenchymal stem cells can inhibit the biological functions of melanoma A375 cells. ONCOLOGY REPORTS, 2018 (PubMed: 29767256) [IF=3.8]

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Phospho-STAT3 (Ser727) Antibody - #AF3294

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