KIF5B Antibody - #AF7947
| 製品: | KIF5B Antibody |
| カタログ: | AF7947 |
| タンパク質の説明: | Rabbit polyclonal antibody to KIF5B |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 110kDa; 110kD(Calculated). |
| ユニプロット: | P33176 |
| RRID: | AB_2844310 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7947, RRID:AB_2844310.
折りたたみ／展開
Conventional kinesin heavy chain; KIF 5B; KIF5B; Kinesin 1; kinesin 1 (110-120kD); Kinesin 1 heavy chain; Kinesin family member 5B; Kinesin heavy chain; kinesin, heavy chain, ubiquitous; Kinesin-1 heavy chain; Kinesin1; KINH; KINH_HUMAN; KNS 1; KNS; KNS1; Ubiquitous kinesin heavy chain; UKHC;
免疫原
- P33176 KINH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEIDSDDTGGSAAQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKNMARRGHSAQIAKPIRPGQHPAASPTHPSAIRGGGAFVQNSQPVAVRGGGGKQV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P33176 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K10 | Acetylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| K28 | Acetylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| C65 | S-Nitrosylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K166 | Acetylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| Y277 | Phosphorylation | Uniprot | |
| R321 | Methylation | Uniprot | |
| K346 | Acetylation | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| T397 | Phosphorylation | Uniprot | |
| T399 | Phosphorylation | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| T405 | Phosphorylation | Uniprot | |
| T414 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| T451 | Phosphorylation | Uniprot | |
| T463 | Phosphorylation | Uniprot | |
| R464 | Methylation | Uniprot | |
| K505 | Ubiquitination | Uniprot | |
| K514 | Ubiquitination | Uniprot | |
| Y516 | Phosphorylation | Uniprot | |
| K526 | Ubiquitination | Uniprot | |
| K539 | Ubiquitination | Uniprot | |
| K549 | Acetylation | Uniprot | |
| K560 | Acetylation | Uniprot | |
| Y593 | Phosphorylation | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| K602 | Acetylation | Uniprot | |
| K609 | Acetylation | Uniprot | |
| K609 | Ubiquitination | Uniprot | |
| C632 | S-Nitrosylation | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| S645 | Phosphorylation | Uniprot | |
| Y649 | Phosphorylation | Uniprot | |
| K656 | Ubiquitination | Uniprot | |
| K657 | Ubiquitination | Uniprot | |
| S663 | Phosphorylation | Uniprot | |
| K679 | Ubiquitination | Uniprot | |
| K690 | Ubiquitination | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| K761 | Acetylation | Uniprot | |
| K764 | Acetylation | Uniprot | |
| K764 | Ubiquitination | Uniprot | |
| K783 | Ubiquitination | Uniprot | |
| K791 | Ubiquitination | Uniprot | |
| K801 | Ubiquitination | Uniprot | |
| S814 | Phosphorylation | Uniprot | |
| S819 | Phosphorylation | Uniprot | |
| S825 | Phosphorylation | Uniprot | |
| S833 | Phosphorylation | Uniprot | |
| K844 | Ubiquitination | Uniprot | |
| K847 | Ubiquitination | Uniprot | |
| R901 | Methylation | Uniprot | |
| S917 | Phosphorylation | Uniprot | |
| K922 | Ubiquitination | Uniprot | |
| S933 | Phosphorylation | Uniprot | |
| T935 | Phosphorylation | Uniprot | |
| S938 | Phosphorylation | Uniprot | |
| S950 | Phosphorylation | Uniprot | |
| R956 | Methylation | Uniprot |
研究背景
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).
Cytoplasm>Cytoskeleton.
Note: Uniformly distributed between soma and neurites in hippocampal neurons.
Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 and PPP1R42 (By similarity). Interacts with SYBU. Interacts with JAKMIP1. Interacts with PLEKHM2. Interacts with ECPAS. Interacts with ZFYVE27 (By similarity). Found in a complex with OGT, RHOT1, RHOT2 and TRAK1. Interacts with APP (via cytoplasmic domain).
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
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