PASK Antibody - #DF10342
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| 製品: | PASK Antibody |
| カタログ: | DF10342 |
| タンパク質の説明: | Rabbit polyclonal antibody to PASK |
| アプリケーション: | WB |
| 反応性: | Human, Mouse |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 142KD; 143kD(Calculated). |
| ユニプロット: | Q96RG2 |
| RRID: | AB_2844349 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF10342, RRID:AB_2844349.
折りたたみ／展開
DKFZP434O051; DKFZp686P2031; hPASK; KIAA0135; MGC93882; mKIAA0135; PAS domain containing serine/threonine kinase; PAS domain containing serine/threonine protein kinase; PAS domain-containing serine/threonine-protein kinase; PAS kinase; PAS serine/threonine kinase; PAS-kinase; Pask; PASK_HUMAN; PASKIN; STK 37; STK37;
免疫原
Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails (at protein level).
- Q96RG2 PASK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEDGGLTAFEEDQRCLSQSLPLPVSAEGPAAQTTAEPSRSFSSAHRHLSRRNGLSRLCQSRTALSEDRWSSYCLSSLAAQNICTSKLHCPAAPEHTDPSEPRGSVSCCSLLRGLSSGWSSPLLPAPVCNPNKAIFTVDAKTTEILVANDKACGLLGYSSQDLIGQKLTQFFLRSDSDVVEALSEEHMEADGHAAVVFGTVVDIISRSGEKIPVSVWMKRMRQERRLCCVVVLEPVERVSTWVAFQSDGTVTSCDSLFAHLHGYVSGEDVAGQHITDLIPSVQLPPSGQHIPKNLKIQRSVGRARDGTTFPLSLKLKSQPSSEEATTGEAAPVSGYRASVWVFCTISGLITLLPDGTIHGINHSFALTLFGYGKTELLGKNITFLIPGFYSYMDLAYNSSLQLPDLASCLDVGNESGCGERTLDPWQGQDPAEGGQDPRINVVLAGGHVVPRDEIRKLMESQDIFTGTQTELIAGGQLLSCLSPQPAPGVDNVPEGSLPVHGEQALPKDQQITALGREEPVAIESPGQDLLGESRSEPVDVKPFASCEDSEAPVPAEDGGSDAGMCGLCQKAQLERMGVSGPSGSDLWAGAAVAKPQAKGQLAGGSLLMHCPCYGSEWGLWWRSQDLAPSPSGMAGLSFGTPTLDEPWLGVENDREELQTCLIKEQLSQLSLAGALDVPHAELVPTECQAVTAPVSSCDLGGRDLCGGCTGSSSACYALATDLPGGLEAVEAQEVDVNSFSWNLKELFFSDQTDQTSSNCSCATSELRETPSSLAVGSDPDVGSLQEQGSCVLDDRELLLLTGTCVDLGQGRRFRESCVGHDPTEPLEVCLVSSEHYAASDRESPGHVPSTLDAGPEDTCPSAEEPRLNVQVTSTPVIVMRGAAGLQREIQEGAYSGSCYHRDGLRLSIQFEVRRVELQGPTPLFCCWLVKDLLHSQRDSAARTRLFLASLPGSTHSTAAELTGPSLVEVLRARPWFEEPPKAVELEGLAACEGEYSQKYSTMSPLGSGAFGFVWTAVDKEKNKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVLDIFENQGFFQLVMEKHGSGLDLFAFIDRHPRLDEPLASYIFRQLVSAVGYLRLKDIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLMGNPYRGPELEMWSLGVTLYTLVFEENPFCELEETVEAAIHPPYLVSKELMSLVSGLLQPVPERRTTLEKLVTDPWVTQPVNLADYTWEEVFRVNKPESGVLSAASLEMGNRSLSDVAQAQELCGGPVPGEAPNGQGCLHPGDPRLLTS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96RG2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K218 | Acetylation | Uniprot | |
| T307 | Phosphorylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| Y389 | Phosphorylation | Uniprot | |
| S390 | Phosphorylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| K570 | Ubiquitination | Uniprot | |
| S579 | Phosphorylation | Uniprot | |
| S582 | Phosphorylation | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| S667 | Phosphorylation | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| S843 | Phosphorylation | Uniprot | |
| T874 | Phosphorylation | Uniprot | |
| K930 | Ubiquitination | Uniprot | |
| S939 | Phosphorylation | Uniprot | |
| S949 | Phosphorylation | Uniprot | |
| T954 | Phosphorylation | Uniprot | |
| K981 | Ubiquitination | Uniprot | |
| Y999 | Phosphorylation | Uniprot | |
| S1000 | Phosphorylation | Uniprot | |
| T1001 | Phosphorylation | Uniprot | |
| S1003 | Phosphorylation | Uniprot | |
| K1034 | Ubiquitination | Uniprot | |
| K1045 | Ubiquitination | Uniprot | |
| S1057 | Phosphorylation | Uniprot | |
| K1122 | Ubiquitination | Uniprot | |
| S1149 | Phosphorylation | Q96RG2 (PASK) | Uniprot |
| T1161 | Phosphorylation | Q96RG2 (PASK) | Uniprot |
| T1165 | Phosphorylation | Q96RG2 (PASK) | Uniprot |
| K1270 | Ubiquitination | Uniprot | |
| S1273 | Phosphorylation | Q96RG2 (PASK) | Uniprot |
| S1277 | Phosphorylation | Q96RG2 (PASK) | Uniprot |
| S1280 | Phosphorylation | Q96RG2 (PASK) | Uniprot |
| S1287 | Phosphorylation | Q96RG2 (PASK) | Uniprot |
| S1289 | Phosphorylation | Q96RG2 (PASK) | Uniprot |
| T1322 | Phosphorylation | Uniprot | |
| S1323 | Phosphorylation | Uniprot |
PTMs - Q96RG2 酵素として
| Substrate | Site | Source |
|---|---|---|
| P13807 (GYS1) | S641 | Uniprot |
| P52945 (PDX1) | T151 | Uniprot |
| P62753 (RPS6) | S235 | Uniprot |
| P62753 (RPS6) | S236 | Uniprot |
| P68104 (EEF1A1) | T432 | Uniprot |
| Q96RG2 (PASK) | S1149 | Uniprot |
| Q96RG2 (PASK) | T1161 | Uniprot |
| Q96RG2 (PASK) | T1165 | Uniprot |
| Q96RG2 (PASK) | S1273 | Uniprot |
| Q96RG2 (PASK) | S1277 | Uniprot |
| Q96RG2 (PASK) | S1280 | Uniprot |
| Q96RG2 (PASK) | S1287 | Uniprot |
| Q96RG2 (PASK) | S1289 | Uniprot |
研究背景
Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation.
Autophosphorylated on Thr-1161 and Thr-1165. Autophosphorylation is activated by phospholipids.
Cytoplasm. Nucleus.
Note: Localizes in the nucleus of testis germ cells and in the midpiece of sperm tails.
Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails (at protein level).
The protein kinase domain mediates binding to phosphatidylinositol.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
参考文献
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