製品: Kizuna Antibody
カタログ: DF10354
タンパク質の説明: Rabbit polyclonal antibody to Kizuna
アプリケーション: WB
反応性: Human, Mouse
予測: Pig, Xenopus
分子量: 75KD; 75kD(Calculated).
ユニプロット: Q2M2Z5
RRID: AB_2844361

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(86%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
Kizuna Antibody detects endogenous levels of total Kizuna.
RRID:
AB_2844361
引用形式: Affinity Biosciences Cat# DF10354, RRID:AB_2844361.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Centrosomal protein kizuna; DKFZP586H021; HT013; Kiz; KIZ_HUMAN; KIZUNA; MGC102941; MGC141930; NCRNA00153; Plk1s1; Polo-like kinase 1 substrate 1; Uncharacterized protein C20orf19;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MSRTLASAVPLSSPDYYERLGQLQHGLRDSEKKRLDLEKKLYEYNQSDTCRVKLKYVKLKNYLKEICESEKKAHTRNQEYLKRFERVQAHVVHFTTNTEKLQKLKLEYETQIKKMLCSKDSLGLKEELTDEDREKVAVHEGINSGTAMSRGLYQPATIFMGRQMSAILSMRDFSTEHKSPQPTKNFSIPDPHSHRQTAQSSNVTDSCVVQTSNDTQCLNKSDNIDGKASLQIGEKMPVTASVLSEEEQTHCLEIGSNTRHGKSNLSEGKKSAELNSPLRERLSPENRTTDLKCDSSSGSEGEILTREHIEVEEKRASPPVSPIPVSEYCESENKWSQEKHSPWEGVSDHLAHREPKSQKPFRKMQEEEEESWSTSSDLTISISEDDLILESPEPQPNPGGKMEGEDGIEALKLIHAEQERVALSTEKNCILQTLSSPDSEKESSTNAPTREPGQTPDSDVPRAQVGQHVATLKEHDNSVKEEATALLRKALTEECGRRSAIHSSESSCSLPSILNDNSGIKEAKPAVWLNSVPTREQEVSSGCGDKSKKENVAADIPITETEAYQLLKKATLQDNTNQTENRFQKTDASVSHLSGLNIGSGAFETKTANKIASEASFSSSEGSPLSRHENKKKPVINLKSNALWDESDDSNSEIEAALRPRNHNTDDSDDFYD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
86
Xenopus
83
Horse
71
Bovine
71
Sheep
71
Dog
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q2M2Z5 基板として

Site PTM Type Enzyme
K40 Ubiquitination
Y42 Phosphorylation
K64 Ubiquitination
K125 Ubiquitination
S165 Phosphorylation
S179 Phosphorylation
S263 Phosphorylation
S266 Phosphorylation
S276 Phosphorylation
S283 Phosphorylation
S317 Phosphorylation
S321 Phosphorylation
T379 Phosphorylation P53350 (PLK1)
S435 Phosphorylation
S436 Phosphorylation
S439 Phosphorylation
T455 Phosphorylation
S458 Phosphorylation
S478 Phosphorylation
K568 Ubiquitination
S594 Phosphorylation
S600 Phosphorylation
T605 Phosphorylation
S616 Phosphorylation
S618 Phosphorylation
S619 Phosphorylation
S620 Phosphorylation
S623 Phosphorylation
S647 Phosphorylation
S650 Phosphorylation
S652 Phosphorylation
T665 Phosphorylation
S668 Phosphorylation

研究背景

機能:

Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar material around the centriole.

PTMs:

Phosphorylation at Thr-379 by PLK1 is not needed for centrosomal localization or pericentriolar material expansion but is indispensable for spindle-pole stabilization.

細胞の位置付け:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Localizes to centrosomes throughout the cell cycle. After centrosome duplication, it usually remains associated only with the mother centrosome, containing the older mature centriole and particles surrounding it. During prophase, additional particles accumulate around both separating centrosomes. Does not accumulate at the microtubule minus ends, but instead localizes to the centrosomes and centrosome-surrounding area in a microtubule-independent and dependent manner, respectively.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with AKAP9, CEP72, ODF2, PCNT and TUBGCP2.

タンパク質ファミリー:

Belongs to the kizuna family.

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