Phospho-STAT6 (Tyr641) Antibody - #AF3301

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製品: Phospho-STAT6 (Tyr641) Antibody
カタログ: AF3301
タンパク質の説明: Rabbit polyclonal antibody to Phospho-STAT6 (Tyr641)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Horse, Sheep, Rabbit, Dog
分子量: 94kDa; 94kD(Calculated).
ユニプロット: P42226
RRID: AB_2834720

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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-STAT6 (Tyr641) Antibody detects endogenous levels of STAT6 only when phosphorylated at Tyrosine 641.
RRID:
AB_2834720
引用形式: Affinity Biosciences Cat# AF3301, RRID:AB_2834720.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

12S1644; D12S1644; IL 4 STAT; IL-4 Stat; IL4 STAT; Interleukin 4 Induced; Interleukin 4 Induced Transcription Factor IL4 STAT; Signal transducer and activator of transcription 6; Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced; Signal Transducer And Activator Of Transcription 6 Nirs Variant 1; Signal transducer and activator of transcription 6, interleukin 4 induced; STAT 6; STAT interleukin4 induced; STAT, interleukin4 induced; Stat6; STAT6_HUMAN; STAT6B; STAT6C; Transcription factor IL 4 STAT;

免疫原

免疫原:
Uniprot:

P42226(STAT6_HUMAN) >>Visit HPA database.

遺伝子(ID):
STAT6(6778)
タンパク質の説明:
STAT6 transcription factor of the STAT family. Plays a central role in IL4-mediated biological responses. Induces the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4.
タンパク質配列:
MSLWGLVSKMPPEKVQRLYVDFPQHLRHLLGDWLESQPWEFLVGSDAFCCNLASALLSDTVQHLQASVGEQGEGSTILQHISTLESIYQRDPLKLVATFRQILQGEKKAVMEQFRHLPMPFHWKQEELKFKTGLRRLQHRVGEIHLLREALQKGAEAGQVSLHSLIETPANGTGPSEALAMLLQETTGELEAAKALVLKRIQIWKRQQQLAGNGAPFEESLAPLQERCESLVDIYSQLQQEVGAAGGELEPKTRASLTGRLDEVLRTLVTSCFLVEKQPPQVLKTQTKFQAGVRFLLGLRFLGAPAKPPLVRADMVTEKQARELSVPQGPGAGAESTGEIINNTVPLENSIPGNCCSALFKNLLLKKIKRCERKGTESVTEEKCAVLFSASFTLGPGKLPIQLQALSLPLVVIVHGNQDNNAKATILWDNAFSEMDRVPFVVAERVPWEKMCETLNLKFMAEVGTNRGLLPEHFLFLAQKIFNDNSLSMEAFQHRSVSWSQFNKEILLGRGFTFWQWFDGVLDLTKRCLRSYWSDRLIIGFISKQYVTSLLLNEPDGTFLLRFSDSEIGGITIAHVIRGQDGSPQIENIQPFSAKDLSIRSLGDRIRDLAQLKNLYPKKPKDEAFRSHYKPEQMGKDGRGYVPATIKMTVERDQPLPTPELQMPTMVPSYDLGMAPDSSMSMQLGPDMVPQVYPPHSHSIPPYQGLSPEESVNVLSAFQEPHLQMPPSLGQMSLPFDQPHPQGLLPCQPQEHAVSSPDPLLCSDVTMVEDSCLSQPVTAFPQGTWIGEDIFPPLLPPTEQDLTKLLLEGQGESGGGSLGAQPLLQPSHYGQSGISMSHMDLRANPSW

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42226 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S8 Phosphorylation
K9 Ubiquitination
K107 Ubiquitination
K108 Ubiquitination
K124 Ubiquitination
K129 Ubiquitination
K252 Ubiquitination
K277 Ubiquitination
K284 Ubiquitination
K288 Ubiquitination
K307 Ubiquitination
K319 Ubiquitination
K361 Ubiquitination
S407 Phosphorylation Q9UHD2 (TBK1)
K450 Ubiquitination
K504 Ubiquitination
T525 Phosphorylation
K595 Ubiquitination
K613 Ubiquitination
K621 Ubiquitination
K630 Ubiquitination
K636 Ubiquitination
Y641 Phosphorylation Q9UHD2 (TBK1)
T645 Phosphorylation
K647 Ubiquitination
S707 Phosphorylation P45983 (MAPK8)
S756 Phosphorylation
S817 Phosphorylation

研究背景

機能:

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

PTMs:

Tyrosine phosphorylated on Tyr-641 following stimulation by IL4/interleukin-4. Tyrosine phosphorylated following stimulation by IL3/interleukin-3 (By similarity). Dephosphorylation on tyrosine residues by PTPN2 negatively regulates the IL4/interleukin-4 mediated signaling.

Mono-ADP-ribosylated by PARP14.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Translocated into the nucleus in response to phosphorylation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Forms a homodimer or a heterodimer with a related family member (By similarity). Interacts with NCOA1 via its C-terminal LXXLL motif.

タンパク質ファミリー:

Belongs to the transcription factor STAT family.

研究領域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

参考文献

1). MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38569295) [IF=7.9]
2). Poly(ADP-ribose) polymerase family member 14 promotes functional recovery after spinal cord injury through regulating microglia M1/M2 polarization via STAT1/6 pathway. Neural Regeneration Research, 2023 (PubMed: 36751810) [IF=6.1]

Application: WB    Species: Mouse    Sample:

Figure 5 PARP14 deficiency activates the STAT1 pathway but blocks the STAT6 pathway in mice 7 days post-SCI. (A) Representative images and quantitative analysis showing p-STAT1 (Try701)+/Iba1+ immunofluorescence staining. Lv-shPARP14 injection further promoted SCI-induced STAT1 pathway activation. White arrows indicate p-STAT1 (Try701)+ (green, FITC-labeled)/Iba1+ (red, Cy3-labeled, microglia marker) cells. (B) Representative images and quantitative analysis showing p-STAT6 (Tyr641)+/Iba1+ immunofluorescence staining. Lv-shPARP14 injection inhibited SCI-induced STAT6 pathway activation. White arrows indicated p-STAT6 (Tyr641)+ (green, FITC-labeled)/Iba1+ (red, Cy3-labeled) cells. Scale bars: 50 µm. (C) Relative protein levels of p-STAT1 (Try701), and p-STAT6 (Tyr641) in each group were detected by western blot analysis. p-STAT1 (Try701) expression was increased by Lv-shPARP14 injection, but p-STAT6 (Tyr641) expression was decreased by PARP14 silencing. Values are shown as mean ± SD (n = 6). **P < 0.01 (one-way analysis of variance followed by Tukey’s post hoc test). Images were taken from the gray matter ventral horn at the injury site. Spinal cord tissues from the injury site were used for western blot detection. DAPI: 4′,6-Diamidino-2-phenylindole; Iba1: ionized calcium-binding adaptor molecule 1; PARP14: poly(ADP-ribose)polymerase, member 14; SCI: spinal cord injury; STAT1: signal transducer and activator of transcription.
3). Schisandra chinensis Lignans Exert Antidepressant Effects by Promoting BV2 Microglia Polarization toward the M2 Phenotype through the Activation of the Cannabinoid Receptor Type-2–Signal Transducer and Activator of Transcription 6 Pathway. Journal of Agricultural and Food Chemistry, 2022 (PubMed: 36349542) [IF=6.1]
4). HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway. MEDIATORS OF INFLAMMATION, 2019 (PubMed: 31772499) [IF=4.6]
5). Dedicator of Cytokinesis 2 (DOCK2) Silencing Protects Against Cerebral Ischemia/Reperfusion by Modulating Microglia Polarization via the Activation of the STAT6 Signaling Pathway. NEUROSCIENCE, 2022 (PubMed: 35395356) [IF=3.3]
6). TRPM7 modulates macrophage polarization by STAT1/STAT6 pathways in RAW264. 7 cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2020 (PubMed: 33153718) [IF=3.1]

Application: WB    Species: Mouse    Sample: raw264.7 cells

Fig. 4. TRPM7 can mediate the macrophage polarization through transcription factors such as STAT1 and STAT6. (A and C) The protein levels of p-STAT1, STAT1, p-STAT6 and STAT6 were detected by Western blot assay. N ¼ 3; mean ± SD. (B and D) The ratios of p-STAT1 to STAT1 and the ratios of p-STAT6 to STAT6. N ¼ 3; mean ± SD. Control, untreated RAW264.7 cells. 2-APB, raw264.7 cells with 2-APB for 24 h. M1, RAW264.7 cells was stimulated with LPS- IFN-g for 24 h. M1þ2-APB, RAW264.7 cells was pretreated with 2-APB for 2 h and stimulated with LPS-IFN-g for 24 h. M2, RAW264.7 cells was stimulated with IL-4 for 24 h. M2þ2-APB, RAW264.7 cells was pretreated with 2-APB for 2 h and with IL-4 for 24 h. NC, RAW264.7 cells transfected with scrambled sequence. siRNA3, RAW264.7 cells transfected with siRNA3. M1þsiRNA3, RAW264.7 cells transfected with siRNA3-TRPM7 and stimulated with LPS-IFN-g for 24 h. M2þsiRNA3, RAW264.7 cells transfected with TRPM7-siRNA3 and stimulated with IL-4 for 24 h**P < 0.01, ***P < 0.0001 versus control; *P < 0.05, **P < 0.01, versus M1; &P < 0.05 versus M2.
7). Scorpion venom polypeptide governs alveolar macrophage M1/M2 polarization to alleviate pulmonary fibrosis. Tissue and Cell, 2022 (PubMed: 36179453) [IF=2.6]
8). Modulation of Wnt/β-catenin signaling in IL-17A-mediated macrophage polarization of RAW264. 7 cells. BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, 2020 (PubMed: 32578719) [IF=2.3]

Application: WB    Species: mouse    Sample: RAW264.7 cells

Figure 6. |Stat signaling is involved in Wnt3A and IL-17A-mediated macrophage polarization in RAW264.7 cells. A, Representative images of immunoblots of indicated proteins of Stat signaling cascade in cells treated with different conditions. Semi-quantitative analysis of the expression of proteins in (A) showing fold changes of protein abundance of p-STAT1 (B), p-STAT3 (C), BCL-XL (D), p21(E), SOCS3 (F), p-STAT6 (G), and STAT6 (H) in cells treated with Wnt3a-CM and/or XAV939 in the presence or absence of rhIL-17A determined by densitometry assay using ImageJ software Fiji.

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