Phospho-SIN3A (Ser832) Antibody | Affinity Biosciences

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製品:	Phospho-SIN3A (Ser832) Antibody
カタログ:	AF4312
タンパク質の説明:	Rabbit polyclonal antibody to Phospho-SIN3A (Ser832)
アプリケーション:	WB
反応性:	Human, Mouse, Rat
予測:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量:	145kDa; 145kD(Calculated).
ユニプロット:	Q96ST3
RRID:	AB_2844391

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サイズ	価格	在庫状況
100ul	$350	在庫あり
200ul	$450	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat

予測:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(86%)

クローナリティ:

Polyclonal

特異性:

Phospho-SIN3A (Ser832) Antibody detects endogenous levels of SIN3A only when phosphorylated at Ser832.

RRID:

AB_2844391
引用形式: Affinity Biosciences Cat# AF4312, RRID:AB_2844391.

コンジュゲート:

Unconjugated.

精製:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

AW553200; DKFZP434K2235; FLJ90319; Histone deacetylase complex subunit Sin 3a; Histone deacetylase complex subunit Sin3a; KIAA0700; Kiaa4126; mKIAA4126; Paired amphipathic helix protein Sin 3a; Paired amphipathic helix protein Sin3a; Sin 3a; SIN3 homolog A; SIN3 homolog A transcription regulator (yeast); SIN3 homolog A transcription regulator; SIN3 transcription regulator homolog A; Sin3a; SIN3A protein; SIN3A_HUMAN; Transcriptional co repressor Sin 3A; Transcriptional co repressor Sin3A; Transcriptional corepressor Sin3a; Transcriptional regulator SIN3A;

免疫原

免疫原:

Uniprot:

Q96ST3(SIN3A_HUMAN) >>Visit HPA database.

遺伝子(ID):

SIN3A(25942)

発現特異性:

Q96ST3 SIN3A_HUMAN:

Expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions.

タンパク質配列:

Q96ST3 SIN3A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKRRLDDQESPVYAAQQRRIPGSTEAFPHQHRVLAPAPPVYEAVSETMQSATGIQYSVTPSYQVSAMPQSSGSHGPAIAAVHSSHHHPTAVQPHGGQVVQSHAHPAPPVAPVQGQQQFQRLKVEDALSYLDQVKLQFGSQPQVYNDFLDIMKEFKSQSIDTPGVISRVSQLFKGHPDLIMGFNTFLPPGYKIEVQTNDMVNVTTPGQVHQIPTHGIQPQPQPPPQHPSQPSAQSAPAPAQPAPQPPPAKVSKPSQLQAHTPASQQTPPLPPYASPRSPPVQPHTPVTISLGTAPSLQNNQPVEFNHAINYVNKIKNRFQGQPDIYKAFLEILHTYQKEQRNAKEAGGNYTPALTEQEVYAQVARLFKNQEDLLSEFGQFLPDANSSVLLSKTTAEKVDSVRNDHGGTVKKPQLNNKPQRPSQNGCQIRRHPTGTTPPVKKKPKLLNLKDSSMADASKHGGGTESLFFDKVRKALRSAEAYENFLRCLVIFNQEVISRAELVQLVSPFLGKFPELFNWFKNFLGYKESVHLETYPKERATEGIAMEIDYASCKRLGSSYRALPKSYQQPKCTGRTPLCKEVLNDTWVSFPSWSEDSTFVSSKKTQYEEHIYRCEDERFELDVVLETNLATIRVLEAIQKKLSRLSAEEQAKFRLDNTLGGTSEVIHRKALQRIYADKAADIIDGLRKNPSIAVPIVLKRLKMKEEEWREAQRGFNKVWREQNEKYYLKSLDHQGINFKQNDTKVLRSKSLLNEIESIYDERQEQATEENAGVPVGPHLSLAYEDKQILEDAAALIIHHVKRQTGIQKEDKYKIKQIMHHFIPDLLFAQRGDLSDVEEEEEEEMDVDEATGAVKKHNGVGGSPPKSKLLFSNTAAQKLRGMDEVYNLFYVNNNWYIFMRLHQILCLRLLRICSQAERQIEEENREREWEREVLGIKRDKSDSPAIQLRLKEPMDVDVEDYYPAFLDMVRSLLDGNIDSSQYEDSLREMFTIHAYIAFTMDKLIQSIVRQLQHIVSDEICVQVTDLYLAENNNGATGGQLNTQNSRSLLESTYQRKAEQLMSDENCFKLMFIQSQGQVQLTIELLDTEEENSDDPVEAERWSDYVERYMNSDTTSPELREHLAQKPVFLPRNLRRIRKCQRGREQQEKEGKEGNSKKTMENVDSLDKLECRFKLNSYKMVYVIKSEDYMYRRTALLRAHQSHERVSKRLHQRFQAWVDKWTKEHVPREMAAETSKWLMGEGLEGLVPCTTTCDTETLHFVSINKYRVKYGTVFKAP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96ST3 基板として

Q96ST3

Site	PTM Type	Enzyme	Source
S10	Phosphorylation		Uniprot
Y13	Phosphorylation		Uniprot
S23	Phosphorylation		Uniprot
Y41	Phosphorylation		Uniprot
K122	Ubiquitination		Uniprot
K155	Ubiquitination		Uniprot
S166	Phosphorylation		Uniprot
S169	Phosphorylation		Uniprot
T260	Phosphorylation		Uniprot
T266	Phosphorylation		Uniprot
Y272	Phosphorylation		Uniprot
S274	Phosphorylation		Uniprot
S277	Phosphorylation		Uniprot
T284	Phosphorylation		Uniprot
S289	Phosphorylation		Uniprot
Y310	Phosphorylation		Uniprot
K337	Ubiquitination		Uniprot
K343	Ubiquitination		Uniprot
T350	Phosphorylation		Uniprot
K391	Ubiquitination		Uniprot
S421	Phosphorylation		Uniprot
T432	Phosphorylation		Uniprot
T434	Phosphorylation	O14757 (CHEK1)	Uniprot
T435	Phosphorylation		Uniprot
S450	Phosphorylation		Uniprot
S451	Phosphorylation		Uniprot
K457	Ubiquitination		Uniprot
K469	Acetylation		Uniprot
S505	Phosphorylation		Uniprot
Y524	Phosphorylation		Uniprot
T539	Phosphorylation		Uniprot
C551	S-Nitrosylation		Uniprot
Y558	Phosphorylation		Uniprot
K563	Ubiquitination		Uniprot
Y565	Phosphorylation		Uniprot
T574	Phosphorylation		Uniprot
T584	Phosphorylation		Uniprot
K601	Acetylation		Uniprot
K601	Ubiquitination		Uniprot
Y610	Phosphorylation		Uniprot
S641	Phosphorylation		Uniprot
K650	Ubiquitination		Uniprot
K676	Ubiquitination		Uniprot
K697	Ubiquitination		Uniprot
K715	Ubiquitination		Uniprot
K723	Acetylation		Uniprot
K727	Acetylation		Uniprot
K727	Ubiquitination		Uniprot
K737	Methylation		Uniprot
S746	Phosphorylation		Uniprot
K747	Ubiquitination		Uniprot
S748	Phosphorylation		Uniprot
Y757	Phosphorylation		Uniprot
T802	Phosphorylation		Uniprot
S832	Phosphorylation		Uniprot
S860	Phosphorylation		Uniprot
K865	Acetylation		Uniprot
K865	Ubiquitination		Uniprot
K875	Acetylation		Uniprot
K875	Ubiquitination		Uniprot
K934	Acetylation		Uniprot
K934	Ubiquitination		Uniprot
K937	Ubiquitination		Uniprot
S938	Phosphorylation		Uniprot
S940	Phosphorylation		Uniprot
Y958	Phosphorylation		Uniprot
Y959	Phosphorylation		Uniprot
K1053	Ubiquitination		Uniprot
S1089	Phosphorylation		Uniprot
Y1101	Phosphorylation		Uniprot
Y1105	Phosphorylation		Uniprot
S1108	Phosphorylation		Uniprot
T1110	Phosphorylation		Uniprot
T1111	Phosphorylation		Uniprot
S1112	Phosphorylation		Uniprot
K1122	Ubiquitination		Uniprot
K1154	Ubiquitination		Uniprot
S1161	Phosphorylation		Uniprot
K1164	Ubiquitination		Uniprot
K1170	Ubiquitination		Uniprot
Y1178	Phosphorylation		Uniprot
K1219	Ubiquitination		Uniprot
S1231	Phosphorylation		Uniprot
K1232	Ubiquitination		Uniprot
K1271	Ubiquitination		Uniprot

研究背景

Q96ST3_SIN3A_HUMAN

機能:

Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (By similarity). Required for cortical neuron differentiation and callosal axon elongation (By similarity).

PTMs:

SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2.

細胞の位置付け:

Nucleus. Nucleus>Nucleolus.
Note: Recruited to the nucleolus by SAP30L.

組織特異性:

Expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions.

サブユニット構造:

Interacts with ARID4B, BRMS1L, HCFC1, HDAC1, HDAC2, MXI1, SAP30L, SAP130, SFPQ and TOPORS. Interacts with OGT (via TPRs 1-6); the interaction mediates transcriptional repression in parallel with histone deacetylase. Interacts with BAZ2A, MXD1, MXD3, MXD4, MBD2, DACH1, NCOR1, NR4A2, REST, RLIM, SAP30, SETDB1, SMYD2, and SUDS3. Interacts with PHF12 in a complex composed of HDAC1, PHF12 and SAP30 (By similarity). Interacts with TET1; the interaction recruits SIN3A to gene promoters. The large PER complex involved in the histone deacetylation is composed of at least HDAC1, PER2, SFPQ and SIN3A (By similarity). Interacts with KLF11 (By similarity). Interacts with PPHLN1. Found in a complex with YY1, GON4L and HDAC1 (By similarity). Interacts (via PAH2) with FOXK1 (By similarity). Interacts with FOXK2 (By similarity). Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1. Interacts with SINHCAF (By similarity). Interacts with SPHK2.

研究領域

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

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Phospho-SIN3A (Ser832) Antibody - #AF4312

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