Phospho-KIBRA (Ser947) Antibody - #AF4317

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製品: Phospho-KIBRA (Ser947) Antibody
カタログ: AF4317
タンパク質の説明: Rabbit polyclonal antibody to Phospho-KIBRA (Ser947)
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 135kDa; 125kD(Calculated).
ユニプロット: Q8IX03
RRID: AB_2844396

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-KIBRA (Ser947) Antibody detects endogenous levels of KIBRA only when phosphorylated at Ser947.
RRID:
AB_2844396
引用形式: Affinity Biosciences Cat# AF4317, RRID:AB_2844396.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FLJ10865; FLJ23369; HBeAg-binding protein 3; HBEBP3; HBEBP36; KIAA0869; KIBRA; Kidney and brain protein; Protein KIBRA; Protein WWC1; WW and C2 domain containing 1; WW C2 and coiled-coil domain containing 1; WW domain-containing protein 1; WWC1;

免疫原

免疫原:
Uniprot:

Q8IX03(KIBRA_HUMAN) >>Visit HPA database.

遺伝子(ID):
WWC1(23286)
発現特異性:
Q8IX03 KIBRA_HUMAN:

Expressed in mammary epithelial cells and breast cancer cell lines. Found in the luminal epithelium surrounding the ducts in the normal breast. In the brain, expressed in somatodendritic compartment of neurons in the cortex and hippocampus and in the cerebellum it is found in the Purkinje cells and some granule cells (at protein level). Detected in brain, heart, colon and kidney. In the kidney, expressed in glomerular podocytes, in some tubules and in the collecting duct.

タンパク質配列:
MPRPELPLPEGWEEARDFDGKVYYIDHTNRTTSWIDPRDRYTKPLTFADCISDELPLGWEEAYDPQVGDYFIDHNTKTTQIEDPRVQWRREQEHMLKDYLVVAQEALSAQKEIYQVKQQRLELAQQEYQQLHAVWEHKLGSQVSLVSGSSSSSKYDPEILKAEIATAKSRVNKLKREMVHLQHELQFKERGFQTLKKIDKKMSDAQGSYKLDEAQAVLRETKAIKKAITCGEKEKQDLIKSLAMLKDGFRTDRGSHSDLWSSSSSLESSSFPLPKQYLDVSSQTDISGSFGINSNNQLAEKVRLRLRYEEAKRRIANLKIQLAKLDSEAWPGVLDSERDRLILINEKEELLKEMRFISPRKWTQGEVEQLEMARKRLEKDLQAARDTQSKALTERLKLNSKRNQLVRELEEATRQVATLHSQLKSLSSSMQSLSSGSSPGSLTSSRGSLVASSLDSSTSASFTDLYYDPFEQLDSELQSKVEFLLLEGATGFRPSGCITTIHEDEVAKTQKAEGGGRLQALRSLSGTPKSMTSLSPRSSLSSPSPPCSPLMADPLLAGDAFLNSLEFEDPELSATLCELSLGNSAQERYRLEEPGTEGKQLGQAVNTAQGCGLKVACVSAAVSDESVAGDSGVYEASVQRLGASEAAAFDSDESEAVGATRIQIALKYDEKNKQFAILIIQLSNLSALLQQQDQKVNIRVAVLPCSESTTCLFRTRPLDASDTLVFNEVFWVSMSYPALHQKTLRVDVCTTDRSHLEECLGGAQISLAEVCRSGERSTRWYNLLSYKYLKKQSRELKPVGVMAPASGPASTDAVSALLEQTAVELEKRQEGRSSTQTLEDSWRYEETSENEAVAEEEEEEVEEEEGEEDVFTEKASPDMDGYPALKVDKETNTETPAPSPTVVRPKDRRVGTPSQGPFLRGSTIIRSKTFSPGPQSQYVCRLNRSDSDSSTLSKKPPFVRNSLERRSVRMKRPSSVKSLRSERLIRTSLDLELDLQATRTWHSQLTQEISVLKELKEQLEQAKSHGEKELPQWLREDERFRLLLRMLEKRQMDRAEHKGELQTDKMMRAAAKDVHRLRGQSCKEPPEVQSFREKMAFFTRPRMNIPALSADDV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IX03 基板として

Site PTM Type Enzyme
K117 Ubiquitination
Y128 Phosphorylation
S141 Phosphorylation
K154 Acetylation
K161 Ubiquitination
S208 Phosphorylation
S241 Phosphorylation
S255 Phosphorylation
K324 Ubiquitination
K347 Ubiquitination
K379 Ubiquitination
S427 Phosphorylation
S438 Phosphorylation
S523 Phosphorylation
S525 Phosphorylation
T527 Phosphorylation
S530 Phosphorylation
T532 Phosphorylation
S533 Phosphorylation
S535 Phosphorylation
S539 Phosphorylation Q96GD4 (AURKB) , O14965 (AURKA)
S542 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3) , P06493 (CDK1)
S548 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S623 Phosphorylation
S626 Phosphorylation
S651 Phosphorylation
Y781 Phosphorylation
Y786 Phosphorylation
Y788 Phosphorylation
S815 Phosphorylation
S834 Phosphorylation
T837 Phosphorylation
S841 Phosphorylation
S876 Phosphorylation
T893 Phosphorylation
T895 Phosphorylation
S899 Phosphorylation
T901 Phosphorylation
T912 Phosphorylation
S922 Phosphorylation
T923 Phosphorylation
S927 Phosphorylation
T929 Phosphorylation Q15418 (RPS6KA1) , P51812 (RPS6KA3)
S931 Phosphorylation P06493 (CDK1)
Y938 Phosphorylation
S945 Phosphorylation
S947 Phosphorylation Q15418 (RPS6KA1) , P51812 (RPS6KA3)
S962 Phosphorylation
S975 Phosphorylation Q05513 (PRKCZ)
S978 Phosphorylation Q05513 (PRKCZ)
T1006 Phosphorylation
S1081 Phosphorylation

研究背景

機能:

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. Modulates directional migration of podocytes. Acts as a substrate for PRKCZ. Plays a role in cognition and memory performance.

PTMs:

Phosphorylation at Ser-542 and Ser-931 by CDK1 in response to spindle damage stress regulates mitotic exit, these two sites are dephosphorylated by CDC14B.

細胞の位置付け:

Cytoplasm. Cytoplasm>Perinuclear region. Nucleus. Cell projection>Ruffle membrane.
Note: Colocalizes with PRKCZ in the perinuclear region.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in mammary epithelial cells and breast cancer cell lines. Found in the luminal epithelium surrounding the ducts in the normal breast. In the brain, expressed in somatodendritic compartment of neurons in the cortex and hippocampus and in the cerebellum it is found in the Purkinje cells and some granule cells (at protein level). Detected in brain, heart, colon and kidney. In the kidney, expressed in glomerular podocytes, in some tubules and in the collecting duct.

サブユニット構造:

Homodimer. Interacts with DDN. Interacts with DYNLL1 and histone H3. The interaction with DYNLL1 is mandatory for the recruitment and transactivation functions of ESR1 or DYNLL1 to the target chromatin and the interaction with histone H3 ensures proper regulatory interaction of WWC1-DYNLL1-ESR1 complexes with target chromatin. Interacts (via WW domains) with DDR1 (via PPxY motif) in a collagen-regulated manner. Interacts with PRKCZ (via the protein kinase domain). Forms a tripartite complex with DDR1 and PRKCZ, but predominantly in the absence of collagen. Interacts (via the ADDV motif) with PATJ (via PDZ domain 8). Interacts (via WW domains) with SYNPO (via PPxY motifs). Interacts with NF2 and SNX4.

タンパク質ファミリー:

The C2-domain mediates homodimerization. It is a calcium-sensitive lipid-binding domain with preference for PI(3)P.

Belongs to the WWC family. KIBRA subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

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